Antibiotic-resistant Escherichia coli isolated from dairy cows and their surrounding environment on a livestock farm practicing prudent antimicrobial use

Suzuki, Yoshihiro; Hiroki, Hayate; Xie, Hengkun; Nishiyama, Masateru; Sakamoto, Shinsuke H.; Uemura, Ryoko; Nukazawa, Kei; Ogura, Yoshitoshi; Watanabe, Tôru; Kobayashi, Ichiro

doi:10.1016/j.ijheh.2022.113930

Cited by 15 publications

(7 citation statements)

References 39 publications

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“…It has been postulated that tetA genes occurred more easily in the environment as compared to other tetracycline determinants [ 22 ]. This postulate appeared to have been confirmed by reports of [ 7 , 23 , 24 ] whose reports presented a higher occurrence rate of tetA as compared to other tet determinants.…”

Section: Discussionsupporting

confidence: 82%

Tetracycline-Resistant Genes in Escherichia coli from Clinical and Nonclinical Sources in Rivers State, Nigeria

Perewari

Otokunefor

Agbagwa

2022

International Journal of Microbiology

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Background. Monitoring the occurrence of tetracycline resistance and its determinants in both clinical and nonclinical settings is essential in understanding the role played by continuous usage of this drug in animal husbandry and the withdrawal of this drug from clinical practice. Limited information is available on this from our locale. This study, therefore, set out to explore the occurrence of specific tetracycline-resistant genes in Escherichia coli from clinical and nonclinical sources in Rivers State, Nigeria. Methods. Two hundred clinical and nonclinical samples were analyzed for the presence of E. coli using standard phenotypic and genotypic tests. Susceptibility testing was carried out using the Kirby–Bauer disc diffusion method, and specific tetracycline-resistant genes (tetA, tetB, tetG, and tetM) were assayed. Results. Results showed that stool samples had the highest occurrence of E. coli (39, 78%), and soil had the lowest (13, 26%). Tetracycline resistance was observed in 80.7% of total isolates. The tetA genes were the most commonly occurring (n = 80, 89.9%) detected in confirmed E. coli isolates, and tetG, the least commonly occurring (n = 16,18%) of isolates. The combined presence of tetA-tetM was the highest (n = 14, 15.7%), followed by tetA-tetB (n = 13, 14.8%). Conclusion. The present study reports on the occurrence and distribution of four tetracycline-resistant determinants in E. coli from clinical and nonclinical sources in Rivers State, Nigeria. The high-level occurrence of the most commonly occurring tetracycline gene even in nonclinical isolates could be indicative of a potential reservoir of this resistance. And, this could limit the reintroduction of tetracycline even in combination therapy.

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Section: Discussionsupporting

confidence: 82%

Tetracycline-Resistant Genes in Escherichia coli from Clinical and Nonclinical Sources in Rivers State, Nigeria

Perewari

Otokunefor

Agbagwa

2022

International Journal of Microbiology

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“…It's followed by streptomycin (15.57%) and chloramphenicol (4.10%) but not found resistant to aztreonam. Similarly, research in Japan by Suzuki et al (2022), for eight months of the year, E. coli isolates from dairy cows and the environment around them on animal farms displayed an 8.3% (10/120) rate of antibiotic resistance, with tetracycline resistance being frequent in all strains. Other research on raw milk samples by Ansharieta et al (2020) and dairy cow's milk samples by Widodo et al (2022) show domination resistance to E. coli bacteria for tetracycline (17.05% and 13.71%).…”

Section: Discussionmentioning

confidence: 86%

Potential hazards of multidrug resistance Escherichia coli collected from wastewater on dairy farms in East Java, Indonesia

et al. 2023

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Abstract. Widodo A, Lamid M, Effendi MH, Tyasningsih W, Wurlina, Al-Arif MA, Raharjo D, Soeharsono, Khairullah AF, Riwu KHP, Yustinasari LR, Kurniawan SC, Silaen OSM, Benjamin MI, Afnani DA. 2023. Potential hazards of multidrug resistance Escherichia coli collected from wastewater on dairy farms in East Java, Indonesia. Biodiversitas 24: 1900-1907. Escherichia coli bacteria initially reside in the digestive tract of humans and animals. This bacterium is treated with antibiotics, but this treatment may trigger antibiotic resistance. Antibiotic resistance genes found in E. coli can transfer to other bacteria, especially in the case of the emergence of multidrug resistance (MDR) bacteria. E. coli is a significant environmental contaminant on dairy farms and is frequently linked to genes that encode extended-spectrum beta-lactamase. This research focused on investigating the potential hazards of multidrug resistance E. coli collected from wastewater on dairy farms in East Java, Indonesia. The number of research samples used was 139 wastewater samples collected from 125 dairy farms in East Java, Indonesia. First, wastewater samples from dairy farms were isolated into BGLB media, then continued with culture on EMBA media, and identifying E. coli bacteria with TSIA and IMViC biochemical tests. The resistance patterns of E. coli were obtained through disk diffusion antibiotic tests on tetracycline, streptomycin, trimethoprim, chloramphenicol, and aztreonam. The results of the isolation and identification obtained 122 (87.77%) E. coli isolates. Antimicrobial susceptibility test to E. coli isolates showed 14 (11.47%) MDR E. coli isolates. The antimicrobial drug resistance pattern TE-ST-W (tetracycline, streptomycin, trimethoprim) accounted for most MDR E. coli isolates in this study, comprising 10 (36.26%); followed by TE-ST-C (tetracycline, streptomycin, chloramphenicol) with two (6.54%) MDR E. coli and two (6.54%) MDR E.coli isolates were resistant to four antibiotics, with the pattern of resistance to TE-ST-W-C (tetracycline, streptomycin, trimethoprim, chloramphenicol) antibiotic. The discovery of multidrug-resistant E. coli isolated from wastewater on dairy farms in East Java, Indonesia, is a new potential hazard that affects public health and needs solutions. One Health integration is anticipated to be used as a solution.

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“…Among 440 E. coli isolates, the highest prevalence of resistance was observed against antibiotics such as streptomycin (33%), amoxicillin/ clavulanate (23%), and tetracycline (8%). On Sumiyoshi Farm in Japan, which strictly applies antibiotics only to sick cattle, 8.3% of E. obtained from livestock stool and drainage water remained resistant to tetracycline and amoxicillin [17]. In South Africa, ESBL E. coli from stool samples had an intersectional phenotype of resistance to streptomycin, ampicillin, sulphafurazole, tetracycline, and ceftazidime [18].…”

Section: Discussionmentioning

confidence: 99%

Resistance profile ofEscherichia coliisolated from stool, feed, and compost sources to antibiotics in Sukabumi

Paramitadevi,

Priadi,

Rahmatika

et al. 2024

E3S Web of Conf.

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Antibiotic-resistant E. coli is a growing concern in various settings, but environmental studies are rare compared to clinical research on human and animal health. This study aimed to identify the prevalence of E. coli bacteria resistant to different antibiotics in the environment by examining E. coli resistant to cefotaxime isolated from ruminant stool, feed, and compost. The phenotyping test was conducted through antibiotic susceptibility test using Kirby-Bauer disk-diffusion method, followed by the One-Way variance (ANOVA) analysis of the antibiotic susceptibility test results. Of the 12 isolates exposed to cefotaxime, six showed resistance to this antibiotic, and all isolates, including those resistant to cefotaxime, were resistant to eight out of ten types of antibiotics. All isolates had resistance to at least two to five types of antibiotics. The phenotypic pattern between fecal isolates and non-fecal isolates did not differ significantly, except for the antibiotics amoxicillin (p≤0.05) and ampicillin (p≤0.05). The overlapping resistance patterns observed in animal feed, animal stool, and compost suggest a potential link between their microbiological compositions.

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Antibiotic-resistant Escherichia coli isolated from dairy cows and their surrounding environment on a livestock farm practicing prudent antimicrobial use

Cited by 15 publications

References 39 publications

Tetracycline-Resistant Genes in Escherichia coli from Clinical and Nonclinical Sources in Rivers State, Nigeria

Tetracycline-Resistant Genes in Escherichia coli from Clinical and Nonclinical Sources in Rivers State, Nigeria

Potential hazards of multidrug resistance Escherichia coli collected from wastewater on dairy farms in East Java, Indonesia

Resistance profile ofEscherichia coliisolated from stool, feed, and compost sources to antibiotics in Sukabumi

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