Antibiotic‐resistantStaphylococcus aureusstrains of swine origin: molecular typing and susceptibility to oregano (Origanum vulgareL.) essential oil and maqui (Aristotelia chilensis(Molina) Stuntz) extract

Abstract

Aim The molecular typing and the susceptibility of Staphylococcus aureus strains of swine origin to antibiotics, oregano (Origanum vulgare L.) essential oil (EO) and Chilean blackberry maqui (Aristotelia chilensis (Molina) Stuntz) extract were determined. Methods and Results Twenty S. aureus strains of swine origin were subjected to molecular typing, of which six strains were selected for antimicrobial susceptibility testing. The epsilon test (Etest) was used to determine the antibiotic susceptibility. The susceptibility to natural antimicrobials (NAs): oregano EO, maqui extract, thymol (Thy) and carvacrol (Carv), was carried out using the disk diffusion method. The S. aureus strains were genetically diverse. All strains were resistant to at least one class of antibiotic, and two strains were multidrug‐resistant. The minimum inhibitory concentration of oregano EO, Thy and Carv was 0·01–0·04%. Maqui extract did not show antistaphylococcal activity. Conclusions Natural antimicrobials extracted from oregano have an inhibitory activity against S. aureus strains from swine origin, with no effect using maqui extract. Significance and Impact of the Study This study provides information about the characteristics of S. aureus strains of swine origin, and about the potential use of NAs from oregano to enhance the control of antibiotic‐resistant S. aureus strains in the pork supply chain.

Antioxidant and Antibacterial Capacities of Origanum vulgare L. Essential Oil from the Arid Andean Region of Chile and its Chemical Characterization by GC-MS

“…These disks were dried under the biosafety chamber’s flow to evaporate the ethanol and avoid its antimicrobial effect. The essential oil showed inhibitory activity against these pathogens, with diameters between 7 and 16 mm, classified as moderate/mild inhibitory activity compared to those reported by other authors [ 30 , 41 , 42 ]. As observed, almost all bacteria were sensitive to the inhibitory effect of oregano essential oil, except Pantoea agglomerans and Agrobacterium tumefaciens .…”

Thymbra capitata essential oil has a significant antimicrobial activity against methicillin-resistant Staphylococcus aureus pre-formed biofilms

“…Hopefully, natural products, in particular EO, have showed an excellent antimicrobial activity against many pathogens (Lofa et al . 2019; Elcocks et al . 2020).…”

Efecto antimicrobiano del aceite esencial de Orégano frente a Listeria monocytogenes y Staphylococcus aureus.

“…La Tabla 2 indica la CMI y CMB del aceite esencial de orégano; estas concentraciones son altas si lo comparamos con lo obtenido en Chile donde obtuvieron una CMI de 0,04% para S. aureus multirresistente (Lofa et al, 2019). Otro estudio determinó que las CMI y CMB del AO mexicano (Poliomintha longiflora) enfrentado a S. aureus, fue de 0,025% y 0,05% respectivamente.…”