Antibiotic sensitivities of Helicobacter pylori vary at different gastric mucosal sites

Borody, Thomas J.; Clancy, R. L.; Warren, Ef; Surace, Rosa; Brusentsev, S.; Mitchell, H. M.

doi:10.1007/978-94-017-1763-2_39

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…The evidence is supported by Borody et al who demonstrated that the bimodal distribution of clarithromycin resistance of isolates cultured from 4 gastric sites (i.e. antrum, distal body, proximal body and fundus) ranged from <0.016 μg/mL to 256 μg/mL 50 . The recent studies also demonstrated that MIC values for clarithromycin resistance vary at different gastric sites 47 – 49 .…”

Section: Discussionmentioning

confidence: 79%

Antimicrobial susceptibility and clarithromycin resistance patterns of Helicobacter pylori clinical isolates in Vietnam

Quek

Pham

Tran³

et al. 2016

F1000Res

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Helicobacter pylori is a gastric pathogen that causes several gastroduodenal disorders such as peptic ulcer disease and gastric cancer. Eradication efforts of H. pylori are often hampered by antimicrobial resistance in many countries, including Vietnam. Here, the study aimed to investigate the occurrence of antimicrobial resistance among H. pylori clinical isolates across 13 hospitals in Vietnam. The study further evaluated the clarithromycin resistance patterns of H. pylori strains. In order to address the study interests, antimicrobial susceptibility testing, epsilometer test and PCR-based sequencing were performed on a total of 193 strains isolated from patients, including 136 children (3–15 years of age) and 57 adults (19–69 years of age). Antimicrobial susceptibility testing showed that the overall resistance to amoxicillin, clarithromycin, levofloxacin, metronidazole, and tetracycline was 10.4%, 85.5%, 24.4%, 37.8%, and 23.8% respectively. The distribution of minimum inhibitory concentrations (MICs) of clarithromycin-resistant strains was 85.5% with MIC >0.5 μg/mL. The majority of the clarithromycin resistant isolates (135 of 165 subjects) have MICs ranging from 2 μg/mL to 16 μg/mL. Furthermore, sequencing detection of mutations in 23S rRNA gene revealed that strains resistant and susceptible to clarithromycin contained both A2143G and T2182C mutations. Of all isolates, eight clarithromycin-resistant isolates (MIC >0.5 μg/mL) had no mutations in the 23S rRNA gene. Collectively, these results demonstrated that a proportion of clarithromycin-resistant H. pylori strains, which are not related to the 23S rRNA gene mutations, could be potentially related to other mechanisms such as the presence of an efflux pump or polymorphisms in the CYP2C19 gene. Therefore, the present study suggests that providing susceptibility testing prior to treatment or alternative screening strategies for antimicrobial resistance is important for future clinical practice. Further studies on clinical guidelines and treatment efficacy are pivotal for successful eradication of H. pylori infection.

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Section: Discussionmentioning

confidence: 79%

Antimicrobial susceptibility and clarithromycin resistance patterns of Helicobacter pylori clinical isolates in Vietnam

Quek

Pham

Tran³

et al. 2016

F1000Res

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“…In a previous study which recruited patients from Sydney, Australia, biopsies were collected, cultured for H. pylori and bacterial DNA extracted as previously described [ 23 ]. In the present study, 45 single colony isolates from Caucasian Australian patients with FD were included.…”

Section: Methodsmentioning

confidence: 99%

The prevalence of the duodenal ulcer promoting gene (dupA) in Helicobacter pylori isolates varies by ethnic group and is not universally associated with disease development: a case-control study

et al. 2009

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Background: The putative H. pylori pathogenicity-associated factor dupA has been associated with IL-8 induction in vitro, and duodenal ulcer (DU) and gastric cancer (GC) development in certain populations, but this association is inconsistent between studies. We aimed to investigate dupA prevalence in clinical isolates from Sweden, Australia and from ethnic Chinese, Indians and Malays resident in Malaysia and Singapore and to examine the association with DU and GC. In addition we investigated the sequence diversity between isolates from these diverse groups and compared the level of IL-8 secretion in isolates possessing and lacking dupA.

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Antibiotic sensitivities of Helicobacter pylori vary at different gastric mucosal sites

Cited by 2 publications

References 17 publications

Antimicrobial susceptibility and clarithromycin resistance patterns of Helicobacter pylori clinical isolates in Vietnam

Antimicrobial susceptibility and clarithromycin resistance patterns of Helicobacter pylori clinical isolates in Vietnam

The prevalence of the duodenal ulcer promoting gene (dupA) in Helicobacter pylori isolates varies by ethnic group and is not universally associated with disease development: a case-control study

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