Antibiotic sensitivity and <i>in vitro</i> antimicrobial activity of plant extracts to <i>pseudomonas fluorescens</i> isolates collected from diseased fish

Abstract

Studies were conducted to identify Pseudomonas fluorescens isolates from a collection of bacteria isolated from bacterial haemorrhagic septicaemia infected carp and catfish, evaluate their antibiotic sensitivity pattern and screen the antibacterial activity of some medicinal plant extracts against the isolates.. A total of 10 isolates were identified as P. fluorescens by morphological, physiological and biochemical tests.In vitro antibiotic sensitivity test of the P. fluorescens isolates were conducted by disc diffusion method for seven antibiotics where, all of the isolates were found to be sensitive only against streptomycin and gentamycin but, most of the isolates (80%) were found resistant to chloramphenicol (C). Moreover, eighty percent of the isolates showed resistance to multiple antibiotics. A total of 118 plant extracts were screened for their antibacterial activity against the P. fluorescens isolates where the isolates exhibited sensitivity to 30 samples. Leaf extracts of Tamarindus indicus, Terminalia chebula, Citrus aurantifolia, Eugenia caryophyllata and Spondias pinnata were found to inhibit the growth of all of the P. fluorescens isolates.

Bacterial Flora of Koi (Anabas testudineus) Harvested from Ponds and Their Antibiogram

“…was found sensitive to chloramphenicol, gentamycin and ciprofloxacin and resistant to cefalexin and ampicillin. In vitro antibiotic sensitivity test of the P. fluorescens isolates were conducted by disk diffusion method against seven antibiotics where, all of the isolates were found to be sensitive to only against streptomycin and gentamycin but, most of the isolates (80%) were found resistant to chloramphenicol (Foysal et al, 2011). As regards to Aeromonas sp., it was sensitive to ciprofloxacin, chloramphenicol and gentamicin and resistant to cefalexin and ampicillin.…”

Antagonistic activity of antibiotic producing Streptomyces sp. against fish and human pathogenic bacteria

“…As a result, most of the bacteria resistant to multiple antibiotics, causing a crucial threat for the treatment of diseases of human and animals (Bennett 2008). The increasing frequency of multidrug resistant pathogenic bacteria in recent years has created an urgent demand in the pharmaceutical industry for screening of new antibiotics (George 2010;Foysal et al 2011;Sharmeen et al 2012;Fuad et al 2012). The results of present study showed that broad spectrum antibiotic could be developed from the isolates N24, N26, N28 and N47, which could be effective against several human and fish diseases.…”

&lt;i&gt;Psidium Guajava&lt;/i&gt; Leaf Extracts Fed to Mono-sex Nile Tilapia &lt;i&gt;Oreochromis Niloticus&lt;/i&gt; Enhance Immune Response Against &lt;i&gt;Pseudomonas fluorescens&lt;/i&gt;

“…Any water which supports long term survival may contribute to an easy outbreaks of disease Chowdhury and Wakabayashi et al, [17]. In Bangladesh, scientific information about bacterial disease in fish are in preliminary stage while it is scare in the field of Pseudomonas fluorescens which has been denoted as one of important disease causing agents of both farmed and wild fish P. fluorescens were originally described as the causative agent of Bacterial Hemorrhagic Septicemia disease of pond cultured fish Foysal et al, [18]. Certain Pseudomonas species may produce additional types of siderophore, such as pyocyanin by Pseudomonas aeruginosa Lau, et al, [19] and thioquinolobactin by Pseudomonas fluorescens Matthijs et al, [20].…”