Antibiotic sensitivity trends of pseudomonas endophthalmitis in a tertiary eye care center in South India: A 12-year retrospective study

Pan, Utsab; Jain, Aarti; Gubert, Joseph; Kumari, Bibha; Sindal, Manavi D

doi:10.4103/ijo.ijo_1145_19

Cited by 9 publications

(9 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The increasing incidence of multidrug-resistant, Gram-negative bacilli characterized by a rapid fulminant course and poor visual and anatomical outcome despite prompt treatment poses a significant challenge for the treatment of endophthalmitis caused by Pseudomonas aeruginosa [18]. While the landmark Endophthalmitis Vitrectomy Study, conducted between 1990 and 1994, showed the presence of Gram-negative bacteria in only 4% of the total postoperative endophthalmitis cases [19], two studies from India, including one from our institute [1,20], have revealed a higher proportion of Gram-negative isolates in culture-proven endophthalmitis, with Pseudomonas spp. Being the predominant Gram-negative organism in both the studies.…”

Section: Discussionmentioning

confidence: 99%

Multidrug-Resistant Pseudomonas aeruginosa Evokes Differential Inflammatory Responses in Human Microglial and Retinal Pigment Epithelial Cells

et al. 2020

View full text Add to dashboard Cite

Increasing incidences of multidrug-resistant (MDR) pathogens causing endophthalmitis threaten our ability to treat this condition, and the modulation of inflammatory responses by MDR bacteria is not known. In this study, using human microglia and retinal pigment epithelial (RPE) cells, we compare the inflammatory responses of sensitive (S-PA) and multidrug-resistant (MDR-PA) clinical isolates of Pseudomonas aeruginosa. Infected cells were subjected to qPCR analysis, enzyme-linked immunosorbent assay (ELISA), and immunostaining to assess the expression of inflammatory mediators. Both microglia and RPE cells, challenged with S-PA and MDR-PA, induced a time-dependent expression of inflammatory cytokines. Significant differences were observed in expression levels of Toll-like receptors (TLR) TLR4, TLR5, and TLR9 in microglia cells challenged with MDR-PA vs. S-PA. Similarly, mRNA levels of interleukin (IL)-6, tumor necrosis factor (TNF)-α, Interferon (IFN)-γ, and matrix metalloproteinase (MMP)-9 were also higher in MDR-PA-infected cells. At protein levels, upregulation was observed for IL-10 (p = 0.004), IL-8 (p = 0.0006), IL-1β (p = 0.02), and Granulocyte-macrophage colony-stimulating factor (GM-CSF) (p = 0.0006) in cells infected MDR-PA versus S-PA in both microglia and RPE cells; however, the response was delayed in RPE cells. Heatmap and STRING analysis highlighted the existence of a cross-talk between the inflammatory and cytokine-mediated signaling pathways. Our study highlights a differential inflammatory response evoked by MDR vs. sensitive pathogens in retinal cells during endophthalmitis.

show abstract

Section: Discussionmentioning

confidence: 99%

Multidrug-Resistant Pseudomonas aeruginosa Evokes Differential Inflammatory Responses in Human Microglial and Retinal Pigment Epithelial Cells

et al. 2020

View full text Add to dashboard Cite

show abstract

“…In India, bacterial resistance to fluoroquinolones, cephalosporins, carbapenem, Beta-Lactam, and colistin is highly prevalent. The most commonly reported resistant strains are E. coli [ 103 , 108 , 109 ], Salmonella species ( spp. ) [ 110 , 111 ], Shigella spp.…”

Section: A Snapshot Of Amr Surveillance Programs and The Epidemiologymentioning

confidence: 99%

“…[ 104 , 112 , 113 ], Pseudomonas spp. [ 102 , 105 , 108 , 109 ], and Acinetobacter spp. [ 100 , 106 , 107 ].…”

Section: A Snapshot Of Amr Surveillance Programs and The Epidemiologymentioning

confidence: 99%

Surveillance of antimicrobial resistance in low- and middle-income countries: a scattered picture

Iskandar

Molinier

Hallit

et al. 2021

Antimicrob Resist Infect Control

235

144

View full text Add to dashboard Cite

Data on comprehensive population-based surveillance of antimicrobial resistance is lacking. In low- and middle-income countries, the challenges are high due to weak laboratory capacity, poor health systems governance, lack of health information systems, and limited resources. Developing countries struggle with political and social dilemma, and bear a high health and economic burden of communicable diseases. Available data are fragmented and lack representativeness which limits their use to advice health policy makers and orientate the efficient allocation of funding and financial resources on programs to mitigate resistance. Low-quality data means soaring rates of antimicrobial resistance and the inability to track and map the spread of resistance, detect early outbreaks, and set national health policy to tackle resistance. Here, we review the barriers and limitations of conducting effective antimicrobial resistance surveillance, and we highlight multiple incremental approaches that may offer opportunities to strengthen population-based surveillance if tailored to the context of each country.

show abstract

“…The World Health Organization declared P. aeruginosa as a critical priority amongst current pathogens urgently in need of new effective antibiotics [19,20]. A recent study in South India showed a rising resistance to fluoroquinolones, amikacin, and ceftazidime in pseudomonas endophthalmitis, particularly in post-surgical cases [21]. Comparable retrospective studies performed in the United States have not observed a similar increase in resistance yet [14].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Pseudomonas aeruginosa isolates from patients with endophthalmitis using conventional microbiologic techniques and whole genome sequencing

Sengillo

Duker

Hernandez

et al. 2020

J Ophthal Inflamm Infect

View full text Add to dashboard Cite

Purpose To demonstrate antibiotic susceptibility and genomic virulence factor profiles of Pseudomonas aeruginosa isolates from patients with culture-confirmed endophthalmitis. Methods Clinical isolates from patients diagnosed with pseudomonas endophthalmitis were included. Laboratory antibiotic susceptibility testing and whole genome sequencing was performed on all isolates. Results In the current study, 8 patients had vitreous culture-confirmed endophthalmitis due to P. aeruginosa. All isolates were multi-drug resistant but sensitive to ceftazidime and each fluoroquinolone tested. Whole genome sequencing revealed a total of 179 unique genes. The most common type of virulence genes included those involved in adherence and the secretion system. Seven of 8 (88%) isolates were of the cytoinvasive phenotype (exoST) and no isolates contained exoU. Conclusions P. aeruginosa associated endophthalmitis is often multi-drug resistant and demonstrates a variety of virulence factors with those involved in adherence and the secretion system being the most common.

show abstract

Antibiotic sensitivity trends of pseudomonas endophthalmitis in a tertiary eye care center in South India: A 12-year retrospective study

Cited by 9 publications

References 12 publications

Multidrug-Resistant Pseudomonas aeruginosa Evokes Differential Inflammatory Responses in Human Microglial and Retinal Pigment Epithelial Cells

Multidrug-Resistant Pseudomonas aeruginosa Evokes Differential Inflammatory Responses in Human Microglial and Retinal Pigment Epithelial Cells

Surveillance of antimicrobial resistance in low- and middle-income countries: a scattered picture

Characterization of Pseudomonas aeruginosa isolates from patients with endophthalmitis using conventional microbiologic techniques and whole genome sequencing

Contact Info

Product

Resources

About