Antibiotic susceptibilities and plasmid profiles of nosocomial methicillin-resistant Staphylococcus aureus: A retrospective study

Gillespie, Matthew T.; May, John W.; Skurray, Ronald A.

doi:10.1099/00222615-17-3-295

Cited by 48 publications

(47 citation statements)

References 14 publications

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“…wt and no association of this phenotype could be made with any plasmids in these strains. The finding that EmR transcipients from mixed culture transfer experiments between SK 1529 and SK982 lacked extrachromosomal DNA provides support for a chromosomal locus for this determinant as has previously been suggested with other Melbourne isolates (Lyon et al, 1983;Gillespie et al, 1984).…”

Section: Plasmid Composition Of S Aureus and Location Of Resistance mentioning

confidence: 57%

“…Only one isolate in this collection, SK460 from 198 1, displayed high-level gentamicin resistance ( > 4096 mg/L) and was additionally resistant to tobramycin, kanamycin, neomycin and amikacin (GTKNAR); SK460 has been described previously (Lyon et al, 1984a). The aminoglycoside MICs for GTKR, GTKNR and GTKNAR strains in this study were equivalent to those displayed by strains of similar phenotypes isolated from another Melbourne hospital (Gillespie et al, 1984). The MICs of tobramycin, kanamycin and neomycin for TKNR strains were 64, 64 and 128 mg/L, respectively.…”

Section: Characteristics Of S Aureus Isolatesmentioning

confidence: 63%

“…Resistance to erythromycin, kanamycin-neomycin, penicillin, streptomycin, tetracycline, cadmium and mercury is usually mediated by determinants carried by the chromosome in these multiresistant S. aureus strains (Lyon et al, 1983(Lyon et al, , 1984a. In contrast, a study of methicillin-resistant S. aureus strains isolated between 1969 and 1981 from a Melbourne hospital (Gillespie, May and Skurray, 1984), revealed that the determinants for penicillinase production and resistance to the heavy metals and tetracycline were plasmid-borne in strains isolated in 1969.…”

Section: Introductionmentioning

confidence: 99%

“…L-broth and L-agar media, methods for determination of penicillinase production, minimum inhibitory concentrations, antibiotic and heavy metal resistance and plasmid elimination procedures were as described previously (Lyon et al, 1983;Gillespie et al, 1984). Resistance to the quaternary ammonium compounds benzalkonium chloride (Sigma Chemical Company, St Louis, MO 63 178 USA) and cetyltrimethylammonium bromide (BDH Chemicals Ltd, Poole, England) was tested in L-agar at concentrations of 4 and 2 mg/L, respectively.…”

Section: Staphylococcus Aureus Strainsmentioning

confidence: 99%

“…Future studies with the strains described in this paper, and with those in a collection of methicillin-resistant S. aureus we have recently described (Gillespie et al, 1984), should provide further insights into the molecular mechanisms whereby resistance determinants have been progressively sequestered by S. aureus over a period of four decades so enabling these organisms to persist as important nosocomial pathogens in environments of high antibiotic usage.…”

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confidence: 99%

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Antibiotic Resistance in Staphylococcus Aureus Isolated at an Australian Hospital between 1946 and 1981

Gillespie¹,

May²,

Skurray

1985

Journal of Medical Microbiology

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SUMMARY.A total of 517 strains of Staphylococcus aureus isolated at a hospital in Melbourne, Australia between 1946 and 198 1 was examined for resistance to a range of antimicrobial agents and for the presence of plasmid DNA. The use of mixed-culture transfer and restriction endonuclease analysis showed that the determinants for resistance to penicillin and to the heavy metals were carried by several related plasmids of (15-23) x lo6 mol. wt, and that tetracycline resistance was encoded on a plasmid of 2.8 x 1 O6 mol. wt in strains isolated before 1970. These phenotypes were chromosomally encoded in the majority of strains isolated thereafter. Resistance to chloramphenicol throughout the study period was plasmid-mediated. Of five aminoglycoside-resistance phenotypes, one was plasmid-mediated and three were chromosomally encoded. The remaining phenotype, specifying low-level gentamicin resistance, was found to be located on the chromosome of early isolates, but in later strains was borne by an 18 x lo6 mol. wt plasmid which also encoded resistance to quaternary ammonium compounds.

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Section: Plasmid Composition Of S Aureus and Location Of Resistance mentioning

confidence: 57%

Section: Characteristics Of S Aureus Isolatesmentioning

confidence: 63%