Antibiotic Susceptibility of Escherichia coli Isolated from some Drinking Water Sources in Tamale Metropolis of Ghana

Abstract

This is the first report on the antibiotic resistance of Escherichia coli isolated from drinking water samples in Tamale Metropolis of Ghana. Antibiotic susceptibility test was performed using the disc diffusion method and the results interpreted using the Clinical and Laboratory Standards Institute guidelines. A total of 56 water sample Escherichia coli isolates were screened against nine different antibiotics. Overall, 37.90% of the Escherichia coli isolates were resistant, 12.90% were intermediate and 49.21% were susceptible. Resistance to vancomycin (94.64%) and erythromycin (85.71%) was high. Susceptibility to ciprofloxacin (94.64%), gentamicin (91.07%) and ceftrioxine (89.29%) was also high. A relatively higher percentage of the water sample Escherichia coli isolates exhibited intermediate resistance to amoxycillin/clavulanic acid (50%). The Escherichia coli isolates also exhibited 24 antibiotic resistant patterns with the pattern E-VA (erythromycin-vancomycin) and SXT-E-VA-TE (Trimethoprim/sulfamethoxazole-erythromycin-vancomycin-tetracycline) being the commonest (each exhibited by nine different isolates). Multiple antibiotic index (MAR index) ranged from 0.11-0.56. Resistance to five (MAR index of 0.56) and four (MAR index of 0.44) different antibiotics were exhibited by 8 and 21 isolates, respectively. A number of Escherichia coli isolated from different water sources did exhibit the same resistant pattern. This study revealed that Escherichia coli from drinking water sources in Tamale, Metropolis are resistant to some antibiotics. Therefore, the use of antibiotics in animal production and for human treatments in the Metropolis need to be checked and curbed to prevent more isolates from becoming resistant.

Distribution of Antibiotic-Resistant Enterobacteriaceae Pathogens in Potable Spring Water of Eastern Indian Himalayas: Emphasis on Virulence Gene and Antibiotic Resistance Genes in Escherichia coli

“…In India, E. coli isolated from the Ganga river showed resistance to antibiotics cephalosporin (37%), ampicillin (20%), amoxicillin (20%), tetracycline (20%), and ciprofloxacin (5.30%) (Biswas et al, 2015). Similarly, several studies had reported the presence of antibiotic-resistant E. coli in different environmental and clinical samples (Sayah et al, 2005;Ram et al, 2007Ram et al, , 2008Li et al, 2010;Tagoe et al, 2011;Guo et al, 2014Guo et al, , 2017Adzitey et al, 2015;Chen et al, 2017;Muringani, 2017;Amer et al, 2018;Diwan et al, 2018;Igwaran et al, 2018;Lu et al, 2018;Mishra et al, 2018;Mitali et al, 2018;Vital et al, 2018;Amador et al, 2019;Sandhu et al, 2019). Study on distribution of ARGs in the present study reflects that the spring water of Sikkim are reservoirs of multiple ARGs.…”

Prevalence and antibiotic susceptibility of Salmonella spp. from water sources in Tamale, Ghana

“…Water also helps to remove waste, lubricates joints, serve as a major component of blood, and a necessary medium for many chemical reactions that help in the formation of meat, eggs, and other animal and plant foods [4]. Potable water is expected to be odorless, tasteless, free from toxic substances, microbial and chemical contaminations [2,5]. Water is also known to be the dwelling place for most bacteria and other microorganisms which cause a variety of waterborne infections such as cholera, typhoid, bilharzia, and malaria [6,7].…”

Isolation and Molecular Characterization of ETEC and NTEC Escherichia coli from Cattle Farm With Reference to Virulence Marker Gene

“…Adzitey et al, 2015 studies E. coli isolated from drinking water and tap water at Livestock farm, incidence was found to be 60 % (5 out of 3) and 10% (3 out of 25) respectively while in our study E coli was isolated in 68.18% ( …”