Antibiotic susceptibility of Helicobacter pylori in Germany: stable primary resistance from 1995 to 2000

Wolle, K.; Leodolter, Andreas; Malfertheiner, Peter; König, Wolfgang

doi:10.1099/0022-1317-51-8-705

Cited by 75 publications

(76 citation statements)

References 20 publications

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“…6 H. pylori resistance to these antimicrobials may be partially explained by the high prevalence of these bacteria in our population. In our study, similar to that reported by Wolle et al, 17 we found no signiÞ cant differences in the prevalence of seven antibiotics resistance between H. pylori isolates from patients with gastritis and DU. Metronidazole and amoxicillin in combination with a proton pump-inhibitor were used for treatment of H. pylori infection with eradication rate of 80% in Europe.…”

Section: Discussionsupporting

confidence: 81%

“…Overall resistance of H. pylori to tetracycline has been found to be low and is estimated to be less than 2%. 17 However, higher resistance rates, up to 20%, have been reported in other studies from different countries. 9,15 The prevalence of tetracycline resistance of H. pylori isolates in Iran is 20%, 17 but our study shows a low resistance of 4.7% indicating the importance of this drug in eradicating H. pylori.…”

Section: Discussionmentioning

confidence: 77%

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Antimicrobial susceptibility testing of <i>Helicobacter pylori </i>to selected agents by agar dilution method in Shiraz-Iran

Kohanteb¹,

Bazargani²,

Saberi-Firoozi³

et al. 2007

Indian J Med Microbiol

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 77%

Antimicrobial susceptibility testing of <i>Helicobacter pylori </i>to selected agents by agar dilution method in Shiraz-Iran

Kohanteb¹,

Bazargani²,

Saberi-Firoozi³

et al. 2007

Indian J Med Microbiol

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“…In all except one of our isolates resistance to clarithromycin was associated with mutations in the 23S rDNA, with polymorphisms arising predominantly at position 2143. The type of polymorphism did not appear to be associated with the origin of the isolate and the level of resistance (MIC), although a study of polymorphism frequency in H. pylori from patients in Germany, where the incidence of clarithromycin resistance was 2 %, reported more diversity, with the A2143G mutation occurring in 53 % of strains and a mutation at position 2142 in 36 % of strains (Wolle et al, 2002). By contrast, we found no direct association between resistance and strain genotype as defined by presence of cagA or vacA allelic type, although most were cagA positive and vacA s1m1 (a common genotype amongst susceptible isolates as well), which was similar to our findings in isolates from north Wales (Elviss et al, 2004a).…”

Section: Discussionmentioning

confidence: 99%

Helicobacter pylori antibiotic-resistance patterns and risk factors in adult dyspeptic patients from ethnically diverse populations in central and south London during 2000

Elviss

Owen

Breathnach

et al. 2005

Journal of Medical Microbiology

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Surveillance of Helicobacter pylori antibiotic susceptibility from patients in London, the largest metropolitan area in the UK, is limited, despite resistance being a key factor in treatment failure. A two-centre survey was performed over 12 months (1999)(2000) to determine antibiotic-resistance rates of isolates from dyspeptic patients attending endoscopy clinics serving two ethnically diverse central and south London communities. The in vitro antibiotic susceptibilities were determined from disc diffusion and epsilometer (E) tests on 101 H. pylori isolates. Overall resistance rates were 59 % for metronidazole and 11 % for clarithromycin, with 8 % resistance to both antibiotics. Corresponding primary resistance rates were 50 % and 7 %, respectively. High-level-resistance was a feature of 82 % of the metronidazole (MIC > 256 mg l À1 ) -resistant and 55 % of the clarithromycin (MIC > 32 mg l À1 ) -resistant strains. All isolates were susceptible to amoxycillin and tetracycline. No associations between resistance and either the gender or the age of the patients were detected. The main risk for resistance to metronidazole was non-UK birth as comparative rates were 68 % for non-UK vs. 40 % for UK-born individuals. Resistant isolates were genotypically diverse with respect to cagA/vacA type. Four 23S rDNA nucleotide polymorphisms were associated with clarithromycin resistance, mostly (9/11) at A2143G. In conclusion, the high overall metronidazole-resistance rate of 59 % for H. pylori from inner London was twice the rate found in other UK-based studies and was attributed to the higher risk of resistant strains infecting individuals born outside the UK. The need for continued resistance surveillance is indicated to monitor the effects of the 'test and treat' strategy for H. pylori eradication, particularly of isolates from at-risk individuals

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“…The minimal inhibitory concentrations of amoxicillin for 90% (MIC90, μg/mL) of known H pylori strains are lower than 0.03 μg/mL [31,32] , and we found that amoxicillin concentrations in gastric juice and gastric mucosa are far higher than 0.03 μg/mL. The amoxicillin dosage used in rats for the study was derived from dosage requirements in humans.…”

Section: Amoxicillin Concentration In Gastric Mucosa and The Effect Omentioning

confidence: 99%

Transfer and distribution of amoxicillin in the rat gastric mucosa and gastric juice and the effects of rabeprazole

Zheng

Bao

et al. 2010

Acta Pharmacol Sin

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Aim: To investigate the distribution of amoxicillin in the gastric juice and gastric mucosa of rats and to investigate the effects of proton pump inhibitor rabeprazole on amoxicillin concentrations in various compartments. Methods: One hundred and sixty anesthetized rats were divided into five groups, and given intravenously different doses of amoxicillin or amoxicillin and rabeprazole. The pH value and volume of gastric juice was aspirated were measured and separated gastric mucosa was homogenized. The concentrations of amoxicillin in the plasma, gastric juice and gastric mucosa were measured by high performance liquid chromatography (HPLC). Results: The maximum concentrations of amoxicillin in gastric juice and gastric mucosa were significantly lower than those in plasma (P<0.001). Concentrations in the glandular stomach mucosa were higher than those in the forestomach mucosa. Rabeprazole did not significantly change the pharmacokinetic parameters of amoxicillin in the plasma and did not alter gastric antibiotic clearance or the gastric transfer fraction of amoxicillin in gastric juice. However, rabeprazole did increase the amoxicillin concentration and pH value in gastric juice and reduced the volume of the gastric juice. Conclusion: Amoxicillin could penetrate the gastric mucosa and achieve therapeutic concentrations at the target site after transfer from the blood to the stomach. Rabeprazole increased the amoxicillin concentration in gastric juice by decreasing the gastric juice volume but did not affect its concentration in blood or gastric mucosa.

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Antibiotic susceptibility of Helicobacter pylori in Germany: stable primary resistance from 1995 to 2000

Cited by 75 publications

References 20 publications

Antimicrobial susceptibility testing of <i>Helicobacter pylori </i>to selected agents by agar dilution method in Shiraz-Iran

Antimicrobial susceptibility testing of <i>Helicobacter pylori </i>to selected agents by agar dilution method in Shiraz-Iran

Helicobacter pylori antibiotic-resistance patterns and risk factors in adult dyspeptic patients from ethnically diverse populations in central and south London during 2000

Transfer and distribution of amoxicillin in the rat gastric mucosa and gastric juice and the effects of rabeprazole

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