Antibiotic Susceptibility of Staphylococcus Species Isolated in Raw Chicken Meat from Retail Stores

Lika, Erinda; Puvača, Nikola; Jeremić, Dejan; Stanojević, Slobodan; Kika, Tana Shtylla; Çoçoli, Sonila; Frutos, Rosa de

doi:10.3390/antibiotics10080904

Cited by 9 publications

(8 citation statements)

References 60 publications

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“…According to the results of the present study, all S. aureus strains showed resistance against ampicillin, similar to same as a study conducted in Nigeria (Awogbemi et al, 2018). The high tetracycline resistance determined in previous studies was similar to that found in the present study (Kraushaar et al, 2017;Lika et al, 2021). Additionally, high antimicrobial resistance rates were demonstrated against kanamycin 74.41% in accordance with the results obtained in South Africa (Mkize et al, 2017).…”

Section: Discussionsupporting

confidence: 92%

Prevalence and Antibiotic Resistance of Salmonella spp. and Staphylococcus aureus Isolated from Broiler Chicken Meat in Modern and Traditional Slaughterhouses of Morocco

Nacer¹,

Ftouhy²,

Derqaoui³

et al. 2022

WVJ

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Handling and consuming contaminated meat can lead to food poisoning and the acquisition of antibiotic resistance genes. Staphylococcus aureus (S. aureus) and Salmonella spp. are the most isolated bacteria from broiler chicken meat, leading to serious foodborne diseases. The present study aimed to evaluate the presence and antibiogram profiles of Salmonella spp. and S. aureus strains in poultry meat purchased from modern and traditional poultry slaughterhouses in Morocco. Foodborne pathogens, such as Salmonella spp. and S. aureus, were isolated from poultry meat using standard methods and then confirmed by biochemical tests (coagulase, catalase, oxidase, motility and API 20E for further biochemical identification) and an immunological test (serotyping test). The antibiogram of the isolates was determined using the agar diffusion method and interpreted according to the criteria of performance standards for antimicrobial susceptibility testing of the Clinical and Laboratory Standards Institute, Wayne, Pennsylvania, USA. A total of 540 poultry meat samples were collected and treated (360 poultry meat samples from traditional slaughterhouses and 180 poultry meat samples from modern slaughterhouses), out of which 15.92% were S. aureus positive and 7.40% were Salmonella spp. positive. In traditional poultry slaughterhouses, the prevalence rates of Salmonella spp. and S. aureus were 11.11% and 20.55%, respectively. In contrast, Salmonella spp. was not detected in poultry samples of modern poultry slaughterhouses, and the prevalence of S. aureus was 6.66%. All S. aureus and 97% of Salmonella spp. isolates were found resistant to at least one antibiotic, while 86% of S. aureus and 30% of Salmonella spp. showed resistance to more than three antibiotics. The obtained results of the present study confirmed that broiler chicken meat purchased from traditional poultry slaughterhouses was mainly contaminated by Salmonella spp. and S. aureus, indicating a major public health risk in Morocco. Therefore, considerable efforts should be made to apply appropriate hygiene practices.

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Section: Discussionsupporting

confidence: 92%

Prevalence and Antibiotic Resistance of Salmonella spp. and Staphylococcus aureus Isolated from Broiler Chicken Meat in Modern and Traditional Slaughterhouses of Morocco

Nacer¹,

Ftouhy²,

Derqaoui³

et al. 2022

WVJ

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“…S. aureus strains may also be transmitted to humans through the food chain via the fecal-oral route [ 23 ]. Studies have shown a high prevalence of staphylococci in food, such as, cheese and meat [ 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

Biofilm Formation of Staphylococcus aureus from Pets, Livestock, and Wild Animals: Relationship with Clonal Lineages and Antimicrobial Resistance

et al. 2022

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This study aimed to compare the biofilm formation ability of Staphylococcus aureus isolated from a wide range of animals and study the association between biofilm formation and antimicrobial resistance and genetic lineages. A total of 214 S. aureus strains isolated from pets, livestock, and wild animals were evaluated regarding their ability to form biofilms by the microtiter biofilm assay and their structure via confocal scanning laser microscopy. Statistical analysis was used to find an association between biofilm formation and antimicrobial resistance, multidrug resistance, sequence types (STs), spa and agr-types of the isolates. The antimicrobial susceptibility of 24 h-old biofilms was assessed against minimum inhibitory concentrations (MIC) and 10× MIC of amikacin and tetracycline, and the biomass reduction was measured. The metabolic activity of biofilms after antimicrobial treatment was evaluated by the XTT assay. All isolates were had the ability to form biofilms. Yet, significant differences in biofilm biomass production were detected among animal species. Multidrug resistance had a positive association with biofilm formation as well as methicillin-resistance. Significant differences were also detected among the clonal lineages of the isolates. Both tetracycline and amikacin were able to significantly reduce the biofilm mass. However, none of the antimicrobials were able to eradicate the biofilm at the maximum concentration used. Our results provide important information on the biofilm-forming capacity of animal-adapted S. aureus isolates, which may have potential implications for the development of new biofilm-targeted therapeutics.

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“…N = Number of expected samples; Z 1-a/2 = Standard normal variant at 5% type I error (P< 0.05); P = prevalence expected based on previous studies [1.3% (Bernier-Lachance et al, 2020), 9.89% (Baghbaderani et al, 2020), 13.9% (Abbasi et al, 2021), 20.5 (Li et al, 2019), 29.1% (Rortana et al, 2021), 35.4% (Tegegne et al, 2021), 66.67% (Savariraj et al, 2020), 89.5% (Lika et al, 2021)]; d = Complete precision or error (which is 5%). Thus, the expected size of the sample was ≥351.…”

Section: Sample Collectionmentioning

confidence: 99%

Prevalence, multiple antibiotic resistance and virulence profile of methicillin-resistant Staphylococcus aureus (MRSA) in retail poultry meat from Edo, Nigeria

Igbinosa

Beshiru

Igbinosa

et al. 2023

Front. Cell. Infect. Microbiol.

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IntroductionStaphylococcus aureus causes staphylococcal food poisoning and several difficult-to-treat infections. The occurrence and dissemination of methicillin-resistance S. aureus (MRSA) in Nigeria is crucial and well documented in hospitals. However, findings on MRSA from meat in the country are yet to be adequately reported. The current study determined the prevalence, virulence profile and antibiogram characteristics of MRSA from a raw chicken product from retail outlets within Edo.MethodsA total of 368 poultry meat samples were assessed for MRSA using a standard culture-based approach and characterized further using a molecular method. The antimicrobial susceptibility profile of the isolates was determined using the disc diffusion method. The biofilm profile of the isolates was assayed via the crystal violet microtitre-plate method. Virulence and antimicrobial resistance genes were screened using polymerase chain reaction via specific primers.ResultsOf the samples tested, 110 (29.9%) were positive for MRSA. All the isolates were positive for deoxyribonuclease (DNase), coagulase and beta-hemolysis production. Biofilm profile revealed 27 (24.55%) weak biofilm formers, 18 (16.36%) moderate biofilm formers, and 39 (35.45%) strong biofilm formers. The isolates harboured 2 and ≤17 virulence genes. Enterotoxin gene profiling revealed that 100 (90.9%) isolates harboured one or more genes. Resistance against the tested antibiotics followed the order: tetracycline 64(58.2%), ciprofloxacin 71(64.6%), trimethoprim 71(64.6%) and rifampin 103(93.6%). A total of 89 isolates were multidrug-resistant, while 3 isolates were resistant to all 22 antibiotics tested. The isolates harboured antimicrobial-resistant determinants such as methicillin-resistant gene (mecA), tetracycline resistance genes (tetK, tetL), erythromycin resistance genes (ermA, ermC), trimethoprim resistance gene (dfrK). All the staphylococcal cassette chromosome mec (SCCmec) IVa and SCCmec V positive isolates harboured the Panton-Valentine Leukocidin Gene (PVL).ConclusionIn conclusion, S. aureus was resistant to commonly used antibiotics; a concern to public health concerning the transmission of these pathogens after consuming these highlight the significance of antimicrobial and enterotoxigenic monitoring of S. aureus in food chains.

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Antibiotic Susceptibility of Staphylococcus Species Isolated in Raw Chicken Meat from Retail Stores

Cited by 9 publications

References 60 publications

Prevalence and Antibiotic Resistance of Salmonella spp. and Staphylococcus aureus Isolated from Broiler Chicken Meat in Modern and Traditional Slaughterhouses of Morocco

Prevalence and Antibiotic Resistance of Salmonella spp. and Staphylococcus aureus Isolated from Broiler Chicken Meat in Modern and Traditional Slaughterhouses of Morocco

Biofilm Formation of Staphylococcus aureus from Pets, Livestock, and Wild Animals: Relationship with Clonal Lineages and Antimicrobial Resistance

Prevalence, multiple antibiotic resistance and virulence profile of methicillin-resistant Staphylococcus aureus (MRSA) in retail poultry meat from Edo, Nigeria

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