Antibiotic Susceptibility Pattern and Distribution of Virulence Factors Among Klebsiella pneumoniae Isolated from Healthy Volunteers

Dalir, Amine; Razavi, Shabnam; Talebi, Malihe; Jazi, Faramarz Masjedian; Bialvaei, Abed Zahedi; Mirshekar, Maryam; Lohrasbi, Vahid

doi:10.30699/ijmm.15.6.676

“…In addition, the pathogenicity of K. pneumoniae is due to various pathogenic factors that enable the bacteria to overcome the host's innate immunity and cause infection in the host. K. pneumoniae virulence factors include lipopolysaccharides, capsule, bio lm formation ability, excessive mucosal viscosity, iron absorption systems, serum resistance, and adhesins (Dalir et al 2021;Zhu et al 2021). standard, they were cultured on Mueller Hinton agar culture medium using sterile swabs.…”

Section: Introductionmentioning

confidence: 99%

Antibiotic resistance and its correlation with biofilm formation and virulence genes in Klebsiella pneumoniae isolated from wounds

Allami

2024

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Klebsiella pneumoniae is the most important species of the Klebsiella genus and often causes hospital infections. These bacteria have a high resistance to most of the available drugs, which has caused concern all over the world. In this study, we investigated the antibiotic resistance profile and the ability to produce Extended-spectrum beta-lactamase (ESBL) among K. pneumoniae isolates, then we investigated the relationship between these two factors with biofilm formation and the prevalence of different virulence genes. In this study, 130 isolates of K. pneumoniae isolated from wounds were investigated. The antibiotic resistance of the isolates was evaluated by the disk diffusion method. The microtiter plate method was used to measure biofilm formation. The prevalence of virulence genes was detected by multiplex PCR.Among the examined isolates, 85.3% showed multidrug resistance. 87.6% of the isolates were ESBL-positive. Imipenem, Meropenem, and Fosfamycin were the most effective drugs. The ability of the isolates to produce biofilm was strong (80%), moderate (12.3%), and weak (7.6%), respectively. FimH, mrKD, entB and tolC virulence genes were observed in all isolates.High prevalence of antibiotic resistance (especially multidrug resistance), high prevalence of ESBL-producing isolates, the ability of all isolates to form a biofilm (especially strong biofilm) and the presence of FimH, mrKD, entB and tolC virulence genes in all isolate show the importance of these factors in the pathogenesis of K. pneumoniae isolates in Iraq.

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Antibiotic resistance and its correlation with biofilm formation and virulence genes in Klebsiella pneumoniae isolated from wounds

Allami,

Mohammed,

Alnaji

et al. 2024

J Appl Genetics

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Klebsiella pneumoniae is the most important species of the Klebsiella genus and often causes hospital infections. These bacteria have a high resistance to most of the available drugs, which has caused concern all over the world. In this study, we investigated the antibiotic resistance pro le and the ability to produce Extended-spectrum beta-lactamase (ESBL) among K. pneumoniae isolates, then we investigated the relationship between these two factors with bio lm formation and the prevalence of different virulence genes. In this study, 130 isolates of K. pneumoniae isolated from wounds were investigated. The antibiotic resistance of the isolates was evaluated by the disk diffusion method. The microtiter plate method was used to measure bio lm formation.The prevalence of virulence genes was detected by multiplex PCR.Among the examined isolates, 85.3% showed multidrug resistance. 87.6% of the isolates were ESBL-positive. Imipenem, Meropenem, and Fosfamycin were the most effective drugs. The ability of the isolates to produce bio lm was strong (80%), moderate (12.3%), and weak (7.6%), respectively. FimH, mrKD, entB and tolC virulence genes were observed in all isolates.High prevalence of antibiotic resistance (especially multidrug resistance), high prevalence of ESBL-producing isolates, the ability of all isolates to form a bio lm (especially strong bio lm) and the presence of FimH, mrKD, entB and tolC virulence genes in all isolate show the importance of these factors in the pathogenesis of K. pneumoniae isolates in Iraq.Despite the high prevalence of infections caused by K. pneuminae in Iraq, a comprehensive study in which the characteristics of this bacterium in terms of drug resistance, beta-lactamase enzyme production, bio lm formation and coding of virulence genes have not been investigated. This study aimed to investigate the factors involved in increasing the resistance and pathogenesis of K. pneuminae. Material and methods Identi cation of bacteria:This study analyzed 130 K. pneuminae isolated from wound swab samples collected from Ibn Al Katteeb hospitals, Al-Kindi Teaching Hospital and private medical clinics in Baghdad between February 2019 and April 2021. Among the patients, 81 were women between 6 and 64 years old (median,53 years) and 49 were male patients between the ages of 8 and 71 years (median,49 years). The samples were cultured on MacConkey agar medium. After 24 − 18 incubation at 37°C, the grown K. pneumoniae isolates were con rmed by microbiological methods, including gram staining and microscopic observation of bacilli, as well as biochemical tests (API-20E test strip) (bioMerieux, France). To de nitively con rm K. pneumoniae in the studied isolates, a PCR test was performed to trace the 16S rRNA gene. Antibiotic resistance pro leThe pattern of antibiotic resistance in the isolates was done using the disk diffusion method according to CLSI (2020) criteria. The isolates were cultured in Brain Heart Infusion (BHI) medium and incubated at 37°C for 18-24 hours. After the growth of bacteria and cre...

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Prevalence of Tetracycline Resistance Genes tet (A, B, C, 39) in Klebsiella pneumoniae Isolated from Tehran, Iran

Ahmadi¹,

Noormohammadi²,

Ranjbar³

et al. 2022

Iran J Med Microbiol

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Background and Aim: Klebsiella pneumoniae is one of the three pathogens that has become a global disease control and treatment problem due to its resistance to common antibiotics. For this reason, it is crucial to study the genes that cause antibiotic resistance in it. Therefore, the aim of this study was to investigate the phenotypic and genotypic frequency of tetracycline resistance in clinical isolates of K. pneumoniae in Tehran, Iran. Materials and Methods:In this study, 100 isolates of K. pneumoniae isolated from clinical samples (urine) during 2018-2019 were studied. In addition to microbial and biochemical phenotypic tests, genotypic tests were conducted to determine the frequency of antibiotic resistance genes tet (A, B, C, 39).Results: Out of 100 isolates of K. pneumoniae, 49 isolates were resistant to tetracyclines. The results of multiplex PCR showed that 31 samples were positive for tetA gene, 8 isolates for tetB gene, 21 samples for tetC and, and 8 isolates for tet39. None of the isolates were positive for all four tetracycline genes. Conclusion:The results of this study showed that the isolates were positive for at least one gene and at most 2 tetracycline resistance genes. The tetA gene showed the highest frequency and the lowest frequency was demonstrated by tetB. The highest binary combination of genes was tetA-tetC, and the lowest was tetA-tet39.

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Antibiotic Susceptibility Pattern and Distribution of Virulence Factors Among Klebsiella pneumoniae Isolated from Healthy Volunteers

Cited by 3 publications

References 27 publications

Antibiotic resistance and its correlation with biofilm formation and virulence genes in Klebsiella pneumoniae isolated from wounds

Antibiotic resistance and its correlation with biofilm formation and virulence genes in Klebsiella pneumoniae isolated from wounds

Antibiotic resistance and its correlation with biofilm formation and virulence genes in Klebsiella pneumoniae isolated from wounds

Prevalence of Tetracycline Resistance Genes tet (A, B, C, 39) in Klebsiella pneumoniae Isolated from Tehran, Iran

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