Antibiotic susceptibility pattern of fish pathogens: A new approach of emerging the bacterial resistance through biofilm formation in in-vitro condition

Acharjee, Mrityunjoy; Hoque, Rezaul; Shreya, Shawda Shafiq; Tabassum, Nafisa; Acharjee, Mahima Ranjan; Rezanujjaman, Md.; ., M. Rahman; Amin, Al; Mahmud, Md. Rayhan

doi:10.1016/j.sjbs.2021.07.063

Cited by 5 publications

(2 citation statements)

References 37 publications

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“…The biofilm lifestyle protects the micro-organisms from environmental stressors (Belkaid & Hand, 2014). Bacteria become more resistant to the same antibiotics when they are part of the biofilm, inhibiting the penetration of the antibiotics into the biofilm structure (Acharjee et al, 2021), which can slow the growth rate within the biofilm, resulting in a genetic selection of resistant strains (Costerton et al, 1999). Furthermore, Streptococcus iniae is already resistant to a wide range of antimicrobials, including FLO, when the bacterium is part of a biofilm (Heckman & Soto, 2021).…”

Section: Re Sults and Discussionmentioning

confidence: 99%

Subinhibitory concentrations of florfenicol increase the biofilm formation of Piscirickettsia salmonis

Oliver

Céspedes

Santibáñez

et al. 2023

Journal of Fish Diseases

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Piscirickettsia salmonis is a Gram-negative facultative intracellular γ-proteobacterium, classified into two genogroups: LF-89 T (ATCC VR-1361) and EM-90 strains (Nourdin-Galindo et al., 2017). This bacterium is the aetiological agent of piscirickettsiosis, a systemic fish disease characterized by the bacterial colonization of several organs, including liver, kidney, spleen, skeletal muscle, intestine and gills among others (Rozas & Enríquez, 2014), causing severe economic losses in Chilean salmonid farms (Sernapesca, 2022a). Although the main disease outbreaks and the subsequent associated mortality had been identified in southern Chile, this disease has also been reported in several countries (Rozas & Enríquez, 2014). The Chilean salmon industry uses several biosecurity measures as a way to mitigate the impact of infectious diseases. Thus, 29 different vaccine formulations are currently approved for use against piscirickettsiosis (SAG, 2022) Statistical data from The Chilean National Fisheries and Aquaculture Service (SERNAPESCA) indicated that piscirickettsiosis is the most prevalent bacterial disease affecting Chilean salmon (Sernapesca, 2022a). For this reason, antibiotics remain the primary treatment against piscirickettsiosis outbreaks. The Chilean salmon industry used 4634 tons of antibiotics in

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Section: Re Sults and Discussionmentioning

confidence: 99%

Subinhibitory concentrations of florfenicol increase the biofilm formation of Piscirickettsia salmonis

Oliver

Céspedes

Santibáñez

et al. 2023

Journal of Fish Diseases

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“…Some bacterial pathogens, such as Escherichia sp., Klebsiella sp., Staphylococcus sp., Proteus sp., and Pseudomonas sp., were isolated from fish and can indicate multiple sources of contamination [ 81 – 83 ]. All LAB isolates showed strong growth inhibition of all reference pathogens: P. aeruginosa , E. coli , S. aureus , P. mirabilis , and K. pneumoniae .…”

Section: Discussionmentioning

confidence: 99%

Functional properties of lactic acid bacteria isolated from Tilapia (Oreochromis niloticus) in Ivory Coast

et al. 2023

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Background Probiotics have recently been applied in aquaculture as eco-friendly alternatives to antibiotics to improve fish health, simultaneously with the increase of production parameters. The present study aimed to investigate the functional potential of lactic acid bacteria (LAB) isolated from the gut of Tilapia (Oreochromis niloticus) originating from the aquaculture farm of Oceanologic Research Center in Ivory Coast. Results Twelve LAB strains were identified by 16 S rDNA gene sequence homology analysis belonging to two genera Pediococcus (P. acidilactici and P. pentosaceus) and Lactobacillus (L. plantarum) with a predominance of P. acidilactici. Several aspects including functional, storage, and safety characteristics were taken into consideration in the selection process of the native LAB isolates as potential probiotics. All LAB isolates showed high antagonistic activity against bacterial pathogens like Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus mirabilis, and Staphylococcus aureus. In addition, the LAB isolates exhibited different degrees of cell surface hydrophobicity in the presence of hexane, xylene, and chloroform as solvents and a good ability to form biofilm. The strong antioxidant activity expressed through the DPPH scavenging capacity of LAB intact cells and their cell-free supernatants was detected. LAB strains survived between 34.18% and 49.9% when exposed to low pH (1.5) and pepsin for 3 h. In presence of 0.3% bile salts, the growth rate ranged from 0.92 to 21.46%. Antibiotic susceptibility pattern of LAB isolates showed sensitivity or intermediate resistance to amoxicillin, cephalothin, chloramphenicol, imipenem, kanamycin, penicillin, rifampicin, streptomycin, tetracycline and resistance to oxacillin, gentamicin, and ciprofloxacin. No significant difference in antibiotic susceptibility pattern was observed between P. acidilactici and P. pentosaceus strains. The non-hemolytic activity was detected. Following the analysis of the enzyme profile, the ability of LAB isolates to produce either lipase or β-galactosidase or both enzymes was highlighted. Furthermore, the efficacy of cryoprotective agents was proved to be isolate-dependent, with LAB isolates having a high affinity for D-sorbitol and sucrose. Conclusion The explored LAB strains inhibited the growth of pathogens and survived after exposure to simulated gastrointestinal tract conditions. The safety and preservative properties are desirable attributes of these new probiotic strains hence recommended for future food and feed applications.

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Dietary inclusion of Pediococcus acidilactici probiotic promoted the growth indices, hemato-biochemical indices, enzymatic profile, intestinal and liver histomorphology, and resistance of Nile Tilapia against Aspergillus flavus

Eissa,

Alaryani,

Elbahnaswy

et al. 2023

Animal Feed Science and Technology

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Antibiotic susceptibility pattern of fish pathogens: A new approach of emerging the bacterial resistance through biofilm formation in in-vitro condition

Cited by 5 publications

References 37 publications

Subinhibitory concentrations of florfenicol increase the biofilm formation of Piscirickettsia salmonis

Subinhibitory concentrations of florfenicol increase the biofilm formation of Piscirickettsia salmonis

Functional properties of lactic acid bacteria isolated from Tilapia (Oreochromis niloticus) in Ivory Coast

Dietary inclusion of Pediococcus acidilactici probiotic promoted the growth indices, hemato-biochemical indices, enzymatic profile, intestinal and liver histomorphology, and resistance of Nile Tilapia against Aspergillus flavus

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