Antibiotic Susceptibility Profile ofAeromonasSpecies Isolated from Wastewater Treatment Plant

Abstract

This study assessed the prevalence of antibiotic-resistant Aeromonas species isolated from Alice and Fort Beaufort wastewater treatment plant in the Eastern Cape Province of South Africa. Antibiotic susceptibility was determined using the disc diffusion method, and polymerase chain reaction (PCR) assay was employed for the detection of antibiotics resistance genes. Variable susceptibilities were observed against ciprofloxacin, chloramphenicol, nalidixic acid, gentamicin, minocycline, among others. Aeromonas isolates from both locations were 100% resistant to penicillin, oxacillin, ampicillin, and vancomycin. Higher phenotypic resistance was observed in isolates from Fort Beaufort compared to isolates from Alice. Class A pse1 β-lactamase was detected in 20.8% of the isolates with a lower detection rate of 8.3% for blaTEM gene. Class 1 integron was present in 20.8% of Aeromonas isolates while class 2 integron and TetC gene were not detected in any isolate. The antibiotic resistance phenotypes observed in the isolates and the presence of β-lactamases genes detected in some isolates are of clinical and public health concern as this has consequences for antimicrobial chemotherapy of infections associated with Aeromonas species. This study further supports wastewater as potential reservoirs of antibiotic resistance determinants in the environment.

Aeromonas hydrophila and Aeromonas veronii Predominate among Potentially Pathogenic Ciprofloxacin- and Tetracycline-Resistant Aeromonas Isolates from Lake Erie

“…Instead, over 98% of tetracycline-and ciprofloxacin-resistant isolates were identified as A. hydrophila or A. veronii, and 72% of isolates that were resistant to both antibiotics were identified as A. hydrophila. The higher prevalence of tetracycline resistance than ciprofloxacin resistance among Aeromonas organisms is in agreement with other environmental and clinical studies (15,21,29,30) and highlights the antibiotic resistance in the genus, specifically among Aeromonas species that are associated with human disease.…”

Prevalence of antimicrobial resistant Aeromonas in chicken and fish washings

“…Findings of the present study are in corroboration with Schmidt et al (2001) and Igbinosa and Okoh (2012) in terms of detection of class 1 integron but contrast for Tet (C). Both authors could not detect Tet (C) in Aeromonas species; however class 1 integron gene cassette was detected.…”

“…The detection of class 1 integron indicates the potential spread of antimicrobial resistance genes within the Enterobacteriaceae family in the simulated environment. Presence of β-lactamase genes in Aeromonas has been reported by Igbinosa and Okoh (2012) which is an alarming public health concern.…”

Fate of antibiotic resistant cultivable heterotrophic bacteria and antibiotic resistance genes in wastewater treatment processes

“…However, the detection frequency of strA was lower than that reported by Wang et al (2013a). The frequency of TEM gene in Aeromonas isolates (Tables S5 and S6) was lower than that reported by Igbinosa and Okoh (2012). Lu et al (2010) reported that the CTX gene and TEM gene were dominant (22 strains) in ESBL-producing bacteria from an urban river sediment habitat.…”