Antibiotic susceptibility profiles of Mycoplasma hyorhinis strains isolated from swine in Hungary

Bekő, Katinka; Felde, Orsolya; Sulyok, Kinga M.; Kreizinger, Zsuzsa; Hrivnák, Veronika; Kiss, Krisztián; Biksi, Imre; Jerzsele, Ákos; Gyuranecz, Miklós

doi:10.1016/j.vetmic.2018.11.027

Cited by 22 publications

(30 citation statements)

References 16 publications

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“…Lincomycin has been previously described as effective in vitro against M. hyorhinis [12,28]. Conversely, our data suggest the presence of resistant strains of M. hyorhinis against this antimicrobial in the population studied, in agreement with the data published by Bekö et al [20] in Hungarian isolates of this pathogen. M. hyorhinis is a common commensal of the upper respiratory tract of pigs, acting as an opportunist pathogen of immunocompromised animal in a variety of clinical presentation [10].…”

Section: Discussionsupporting

confidence: 93%

“…Freely accessible tylvalosin MIC values for mycoplasmas are limited up to date to M. hyopneumoniae , M. hyorhinis , M. synoviae , Mycoplasma sp. 1220 and M. gallisepticum [ 17 – 20 ]. However, the MIC values observed in our work for this antimicrobial against M. hyorhinis and M. hyosynoviae , and the in vitro [ 17 ] and in vivo efficacy [ 21 ] demonstrated for the treatment of M. hyopneumoniae infections, gives prominence to tylvalosin as one of the most effective macrolides against porcine mycoplasmosis, together with tulathromycin, a 15-membered macrolide.…”

Section: Discussionmentioning

confidence: 99%

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Antimicrobial susceptibility profiles of porcine mycoplasmas isolated from samples collected in southern Europe

et al. 2020

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Background: Mycoplasma (M.) hyopneumoniae, M. hyorhinis and M. hyosynoviae are significant pathogens for the porcine industry worldwide. The aim of the present study was to determine the antimicrobial susceptibility of six key antimicrobials (tylosin, tilmicosin, tylvalosin, lincomycin, tiamulin and valnemulin) routinely used for treating infections caused by these pathogens. Twenty-seven M. hyopneumoniae, 48 M. hyorhinis and 40 M. hyosynoviae field strains isolated from clinical samples from different Southern European countries between 2013 and 2018 using broth microdilution method were evaluated. Results: Tylvalosin exhibited the highest in vitro activity among the macrolides assayed, with MIC 90 values 4 to 5 twofold dilutions lower than those of tylosin and tilmicosin. The pleuromutilin valnemulin showed one of the highest in vitro activities against the three mycoplasma species. On the contrary, lincomycin exhibited the highest MIC values of the antimicrobials tested. Conclusions: The data obtained in the present study supports the use of pleuromutilins and macrolides for the control of infections caused by porcine mycoplasmas. The use of lincomycin for the treatment of porcine mycoplasma infections should be carefully evaluated due to the presence of circulating field isolates with decreased susceptibility to this antimicrobial.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Antimicrobial susceptibility profiles of porcine mycoplasmas isolated from samples collected in southern Europe

et al. 2020

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“…Even though tiamulin is not licensed for cattle in Italy, it was included in this study to investigate its efficacy against M. dispar as a potential targeted treatment. Noteworthy, most of the isolates had low MIC values for tiamulin between 0.25 and 2 µg/mL, arranged unimodally, but overall had slightly higher values than those in previous studies [20,37] as well as for the pig pathogens M. hyorhinis [38] and M. hyopneumoniae [39] for which the antibiotic is licensed. These low values for M. dispar and, incidentally, M. bovis [33] may reflect the absence of exposure of these mycoplasmas to tiamulin which is not used for treating cattle.…”

Section: Discussionmentioning

confidence: 64%

The pathogen Mycoplasma dispar Shows High Minimum Inhibitory Concentrations for Antimicrobials Commonly Used for Bovine Respiratory Disease

et al. 2020

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Mycoplasma dispar is an overlooked pathogen often involved in bovine respiratory disease (BRD), which affects cattle around the world. BRD results in lost production and high treatment and prevention costs. Additionally, chronic therapies with multiple antimicrobials may lead to antimicrobial resistance. Data on antimicrobial susceptibility to M. dispar is limited so minimum inhibitory concentrations (MIC) of a range of antimicrobials routinely used in BRD were evaluated using a broth microdilution technique for 41 M. dispar isolates collected in Italy between 2011–2019. While all isolates had low MIC values for florfenicol (<1 μg/mL), many showed high MIC values for erythromycin (MIC90 ≥8 μg/mL). Tilmicosin MIC values were higher (MIC50 = 32 μg/mL) than those for tylosin (MIC50 = 0.25 μg/mL). Seven isolates had high MIC values for lincomycin, tilmicosin and tylosin (≥32 μg/mL). More, alarmingly, results showed more than half the strains had high MICs for enrofloxacin, a member of the fluoroquinolone class considered critically important in human health. A time-dependent progressive drift of enrofloxacin MICs towards high-concentration values was observed, indicative of an on-going selection process among the isolates.

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“…At present, Mhr infection detection mainly depends on pathogen isolation and culture and polymerase chain reaction (PCR) methods, and there is no commercially available kit for serological detection [6]. Although Mhr is easily isolated from porcine alveolar lavage fluid and nasal swabs, the process of isolation and identification of Mhr is often time consuming [7]. Mhr has been suspected to be associated with several types of cancer, and previous studies have confirmed that Mhr reduces the sensitivity of human lung carcinoma cells to Nutlin-3 and promotes their malignant phenotype.…”

Section: Introductionmentioning

confidence: 99%

Identification of specific B cell linear epitopes of Mycoplasma hyorhinis P37 protein using monoclonal antibodies against baculovirus-expressed P37 protein

Zhu¹,

Wei

Huang

et al. 2019

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Background Mycoplasma hyorhinis (Mhr) is the etiologic agent of lameness and polyserositis in swine. P37 is a membrane protein of Mhr that may be an important immunogen and is a potential target for diagnostic development. However, there is little information concerning Mhr P37 protein epitopes. A precise analysis of the P37 protein epitopes should extend our understanding of the antigenic composition of the P37 protein and the humoral immune responses to Mhr infection. Investigating the epitopes of Mhr P37 will help to establish a detection method for Mhr in tissue and provide an effective tool for detecting Mhr infection. Results Western blot and indirect immunofluorescence assays (IFA) confirmed that the expressed P37 protein was recognized by Mhr-positive porcine and mouse sera. Furthermore, the P37 protein was purified using affinity chromatography and used to immunize mice for hybridoma cell fusion. Four monoclonal antibodies (mAbs) found to be positive for Mhr were detected in infected lung tissue. A panel of truncated P37 proteins was used to identify the minimal B cell linear epitopes of the protein based on these mAbs. The core epitope was determined to be 206 KIKKAWNDKDWNTFRNF 222 . Conclusions In this study, we identified 17 critical amino acids that determine the epitope of the P37 protein of Mhr. This study identified mAbs that could provide useful tools for investigating the Mhr P37 antigenic core epitope (amino acids 206-222) and detecting Mhr-specific antigens in infected tissue.

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Antibiotic susceptibility profiles of Mycoplasma hyorhinis strains isolated from swine in Hungary

Cited by 22 publications

References 16 publications

Antimicrobial susceptibility profiles of porcine mycoplasmas isolated from samples collected in southern Europe

Antimicrobial susceptibility profiles of porcine mycoplasmas isolated from samples collected in southern Europe

The pathogen Mycoplasma dispar Shows High Minimum Inhibitory Concentrations for Antimicrobials Commonly Used for Bovine Respiratory Disease

Identification of specific B cell linear epitopes of Mycoplasma hyorhinis P37 protein using monoclonal antibodies against baculovirus-expressed P37 protein

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