Antibiotics Interfere with the Evolution of Plasmid Stability

Wein, Tanita; Wang, Yiqing; Hülter, Nils; Hammerschmidt, Katrin; Dagan, Tal

doi:10.1016/j.cub.2020.07.019

Cited by 42 publications

(48 citation statements)

References 42 publications

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“…Our results reveal a diversification process of plasmid fusion variants over time that leads to the enrichment of selected variants. Selection for antibiotics resistance may preserve unstable plasmid variants in the population (Wein et al, 2020); our results here show that such unstable plasmids can be maintained in the population over time also under nonselective conditions via plasmid fusion. Plasmid fusion of plasmid alleles conferring resistance to different antibiotics has been invoked as a likely route for the evolution of plasmids carrying multiple antibiotics resistance genes (Condit and Levin, 1990).…”

Section: Discussionmentioning

confidence: 55%

“…Both plasmids originated from the pBBR1 backbone isolated from Bordetella bronchiseptica (Antoine and Locht, 1992); the pBBR1 plasmid is typically characterized by a small genome size and it is widely spread in diverse environments (Wein et al, 2019). The plasmids pCON and pCON-S are non-mobile, encode the kanamycin resistance gene nptII and have a comparable plasmid copy number ranging between 2 and 6 (Wein et al, 2019(Wein et al, , 2020. While pCON is characterized by an unstable inheritance in a population, and accordingly is lost over time, pCON-S is characterized by a stable inheritance in the population over long time scales (Wein et al, 2020).…”

Section: Comparison Of Plasmid Fitness Independent Of the Bacterial Hmentioning

confidence: 99%

“…The plasmids pCON and pCON-S are non-mobile, encode the kanamycin resistance gene nptII and have a comparable plasmid copy number ranging between 2 and 6 (Wein et al, 2019(Wein et al, , 2020. While pCON is characterized by an unstable inheritance in a population, and accordingly is lost over time, pCON-S is characterized by a stable inheritance in the population over long time scales (Wein et al, 2020). The instability of pCON is a result of transcription-replication conflicts of the plasmid replication machinery and the transcription of the nptII gene (Wein et al, 2019) that is resolved by an insertion between the nptII gene and the oriV region in pCON-S (Wein et al, 2020) (see details in Figure 1B) (Wein et al, 2019(Wein et al, , 2020.…”

Section: Comparison Of Plasmid Fitness Independent Of the Bacterial Hmentioning

confidence: 99%

“…While pCON is characterized by an unstable inheritance in a population, and accordingly is lost over time, pCON-S is characterized by a stable inheritance in the population over long time scales (Wein et al, 2020). The instability of pCON is a result of transcription-replication conflicts of the plasmid replication machinery and the transcription of the nptII gene (Wein et al, 2019) that is resolved by an insertion between the nptII gene and the oriV region in pCON-S (Wein et al, 2020) (see details in Figure 1B) (Wein et al, 2019(Wein et al, , 2020. Both pCON and pCON-S have no measurable effect on host fitness (Wein et al, 2019(Wein et al, , 2020.…”

Section: Comparison Of Plasmid Fitness Independent Of the Bacterial Hmentioning

confidence: 99%

“…The instability of pCON is a result of transcription-replication conflicts of the plasmid replication machinery and the transcription of the nptII gene (Wein et al, 2019) that is resolved by an insertion between the nptII gene and the oriV region in pCON-S (Wein et al, 2020) (see details in Figure 1B) (Wein et al, 2019(Wein et al, , 2020. Both pCON and pCON-S have no measurable effect on host fitness (Wein et al, 2019(Wein et al, , 2020. To compete both plasmid variants in the same host, we created pCON2 and pCON-S2 in which we replaced the kanamycin resistance gene nptII with the cat gene that confers resistance to chloramphenicol ( Figure 1B).…”

Section: Comparison Of Plasmid Fitness Independent Of the Bacterial Hmentioning

confidence: 99%

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Intracellular Competitions Reveal Determinants of Plasmid Evolutionary Success

et al. 2020

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Plasmids are autonomously replicating genetic elements that are ubiquitous in all taxa and habitats where they constitute an integral part of microbial genomes. The stable inheritance of plasmids depends on their segregation during cell division and their longterm persistence in a host population is thought to largely depend on their impact on the host fitness. Nonetheless, many plasmids found in nature are lacking a clear trait that is advantageous to their host; the determinants of plasmid evolutionary success in the absence of plasmid benefit to the host remain understudied. Here we show that stable plasmid inheritance is an important determinant of plasmid evolutionary success. Borrowing terminology from evolutionary biology of cellular living forms, we hypothesize that Darwinian fitness is key for the plasmid evolutionary success. Performing intracellular plasmid competitions between non-mobile plasmids enables us to compare the evolutionary success of plasmid genotypes within the host, i.e., the plasmid fitness. Intracellular head-to-head competitions between stable and unstable variants of the same model plasmid revealed that the stable plasmid variant has a higher fitness in comparison to the unstable plasmid. Preemptive plasmid competitions reveal that plasmid fitness may depend on the order of plasmid arrival in the host. Competitions between plasmids characterized by similar stability of inheritance reveal plasmid fitness differences depending on the plasmid-encoded trait. Our results further reveal that competing plasmids can be maintained in coexistence following plasmid fusions that maintain unstable plasmid variants over time. Plasmids are not only useful accessory genetic elements to their host but they are also evolving and replicating entities, similarly to cellular living forms. There is a clear link between plasmid genetics and plasmid evolutionary success-hence plasmids are evolving entities whose fitness is quantifiable.

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Section: Discussionmentioning

confidence: 55%

Section: Comparison Of Plasmid Fitness Independent Of the Bacterial Hmentioning

confidence: 99%

Section: Comparison Of Plasmid Fitness Independent Of the Bacterial Hmentioning

confidence: 99%

Section: Comparison Of Plasmid Fitness Independent Of the Bacterial Hmentioning

confidence: 99%

Section: Comparison Of Plasmid Fitness Independent Of the Bacterial Hmentioning

confidence: 99%

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Intracellular Competitions Reveal Determinants of Plasmid Evolutionary Success

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Colonization of Plastic Marine Debris

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2022

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Mobile and Self‐Sustained Data Storage in an Extremophile Genomic DNA

Sun

Dong

et al. 2023

Advanced Science

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DNA has been pursued as a novel biomaterial for digital data storage. While large-scale data storage and random access have been achieved in DNA oligonucleotide pools, repeated data accessing requires constant data replenishment, and these implementations are confined in professional facilities. Here, a mobile data storage system in the genome of the extremophile Halomonas bluephagenesis, which enables dual-mode storage, dynamic data maintenance, rapid readout, and robust recovery. The system relies on two key components: A versatile genetic toolbox for the integration of 10-100 kb scale synthetic DNA into H. bluephagenesis genome and an efficient error correction coding scheme targeting noisy nanopore sequencing reads. The storage and repeated retrieval of 5 KB data under non-laboratory conditions are demonstrated. The work highlights the potential of DNA data storage in domestic and field scenarios, and expands its application domain from archival data to frequently accessed data.

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Antibiotics Interfere with the Evolution of Plasmid Stability

Cited by 42 publications

References 42 publications

Intracellular Competitions Reveal Determinants of Plasmid Evolutionary Success

Intracellular Competitions Reveal Determinants of Plasmid Evolutionary Success

Colonization of Plastic Marine Debris

Mobile and Self‐Sustained Data Storage in an Extremophile Genomic DNA

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