Antibiotics Sensitivity Profile Towards &lt;i&gt;Staphylococcus hominis&lt;/i&gt; in Assir Region of Saudi Arabia

Abdalla, Nazar M; Haimour, Waleed O; Osman, A. A.; Sarhan, M. A.; Musaa, H. A.

doi:10.3329/jsr.v5i1.11704

Cited by 3 publications

(4 citation statements)

References 14 publications

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“…In Ghana, most bacterial isolates have acquired resistance due to the unregulated purchase of unprescribed antibiotics and/or inappropriate use of these antibiotics. 26 From this study, the S.…”

Section: Discussionmentioning

confidence: 78%

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Antibiotic resistance and mecA characterization of Staphylococcus hominis from filarial lymphedema patients in the Ahanta West District, Ghana: A cross‐sectional study

Kini

Wireko

Osei-Poku

et al. 2023

Health Science Reports

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Background and Aim Filarial infections affect over 150 million people in the tropics. One of the major forms of filarial pathologies is lymphedema; a condition where the immune response is significantly altered, resulting in changes in the normal flora. Staphylococcus hominis , a human skin commensal, can also be pathogenic in immunocompromised individuals. Therefore, there is the possibility that S. hominis could assume a different behavior in filarial lymphedema patients. To this end, we investigated the levels of antibiotic resistance and extent of mec A gene carriage in S. hominis among individuals presenting with filarial lymphedema in rural Ghana. Method We recruited 160 individuals with stages I–VII lymphedema, in a cross‐sectional study in the Ahanta West District of the Western Region of Ghana. Swabs from lymphedematous limb ulcers, pus, and cutaneous surfaces were cultured using standard culture‐based techniques. The culture isolates were subjected to Matrix‐Assisted Laser Desorption/Ionization Time of Flight (MALDI‐TOF) mass spectrometry for bacterial identification. Antimicrobial susceptibility testing (AST) was performed using the Kirby–Bauer method. mec A genes were targeted by polymerase chain reaction for strains that were cefoxitin resistant. Results In all, 112 S . hominis were isolated. The AST results showed resistance to chloramphenicol (87.5%), tetracycline (83.3%), penicillin (79.2%), and trimethoprim/sulphamethoxazole (45.8%). Of the 112 strains of S. hominis , 51 (45.5%) were resistant to cefoxitin, and 37 (72.5%) of the cefoxitin‐resistant S. hominis haboured the mec A gene. Conclusion This study indicates a heightened level of methicillin‐resistant S. hominis isolated among filarial lymphedema patients. As a result, opportunistic infections of S. hominis among the already burdened filarial lymphedema patients in rural Ghana may have reduced treatment success with antibiotics.

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Section: Discussionmentioning

confidence: 78%

“…In Ghana, most bacterial isolates have acquired resistance due to the unregulated purchase of unprescribed antibiotics and/or inappropriate use of these antibiotics 26 . From this study, the S. hominis isolates were resistant to chloramphenicol (87.5%), tetracycline (83.0%), penicillin (79.5%), and trimethoprim/sulphamethoxazole (45.5%).…”

Section: Discussionmentioning

confidence: 80%

Antibiotic resistance and mecA characterization of Staphylococcus hominis from filarial lymphedema patients in the Ahanta West District, Ghana: A cross‐sectional study

Kini

Wireko

Osei-Poku

et al. 2023

Health Science Reports

View full text Add to dashboard Cite

show abstract

“…Staphylococcus hominis is a coagulase-negative member of the bacteria genus Staphylococcus, consisting of Gram-positive, spherical cells in clusters. S. hominis is normally found on human skin of usually harmless people, but it can sometimes cause infection in people with abnormally weak immune systems, their presence in food are indication of excessive human handling (Abdalla et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

Isolation and Identification of Bacteria Associated with Pre and Post Processing of Groundnut Cake in Sokoto State, North-Western Nigeria

2020

JBAH

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This study was conducted to assess the bacterial contamination between the groundnut cakes and paste obtained from Rural areas (Bodinga, kware) and that obtained within the metropolis at Urban areas (Arkilla and 'Yargoriba) of Sokoto metropolis. A total of 8 samples of ready to eat groundnut cake and pastes were purchased, 4 of which were cake packaged in polythene and 4 paste packaged in plastic rubbers. The samples were analyzed microbiologically using standard procedures. Bacteria species associated with the samples collected from the rural areas were identified as Bacillus lentus, B. megaterium, B. licheniformis, Listeria monocytogene, Staphylococcus aureus while those associated with samples collected from the urban areas were identified as Bacillus lentus, B. megaterium, B. licheniformis, Listeria monocytogene, Staphylococcus aureus and S. hominis. There was higher bacteria count on samples from rural areas compared to those from urban areas. This might be as a result of unhygienic practices during the processing of the product which may range from methods of obtaining and quality of raw materials including sources of water, packaging, handling and distribution of the finished product to the final consumers. Based on these, it is recommended that Producers of groundnut cake should use good hygienic places, raw material and equipment to reduce the risk of microbial contamination.

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“…Staphylococcus hominis is a Gram-positive nosocomial pathogen and a member of the staphylococcus genus, consisting of spherical cells [ 1 ]. Despite being a harmless commensal to human and animal skin, it potentially causes bloodstream infections in immunocompromised patients.…”

Section: Introductionmentioning

confidence: 99%

Genome-Based Multi-Antigenic Epitopes Vaccine Construct Designing against Staphylococcus hominis Using Reverse Vaccinology and Biophysical Approaches

et al. 2022

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Staphylococcus hominis is a Gram-positive bacterium from the staphylococcus genus; it is also a member of coagulase-negative staphylococci because of its opportunistic nature and ability to cause life-threatening bloodstream infections in immunocompromised patients. Gram-positive and opportunistic bacteria have become a major concern for the medical community. It has also drawn the attention of scientists due to the evaluation of immune evasion tactics and the development of multidrug-resistant strains. This prompted the need to explore novel therapeutic approaches as an alternative to antibiotics. The current study aimed to develop a broad-spectrum, multi-epitope vaccine to control bacterial infections and reduce the burden on healthcare systems. A computational framework was designed to filter the immunogenic potent vaccine candidate. This framework consists of pan-genomics, subtractive proteomics, and immunoinformatics approaches to prioritize vaccine candidates. A total of 12,285 core proteins were obtained using a pan-genome analysis of all strains. The screening of the core proteins resulted in the selection of only two proteins for the next epitope prediction phase. Eleven B-cell derived T-cell epitopes were selected that met the criteria of different immunoinformatics approaches such as allergenicity, antigenicity, immunogenicity, and toxicity. A vaccine construct was formulated using EAAAK and GPGPG linkers and a cholera toxin B subunit. This formulated vaccine construct was further used for downward analysis. The vaccine was loop refined and improved for structure stability through disulfide engineering. For an efficient expression, the codons were optimized as per the usage pattern of the E coli (K12) expression system. The top three refined docked complexes of the vaccine that docked with the MHC-I, MHC-II, and TLR-4 receptors were selected, which proved the best binding potential of the vaccine with immune receptors; this was followed by molecular dynamic simulations. The results indicate the best intermolecular bonding between immune receptors and vaccine epitopes and that they are exposed to the host’s immune system. Finally, the binding energies were calculated to confirm the binding stability of the docked complexes. This work aimed to provide a manageable list of immunogenic and antigenic epitopes that could be used as potent vaccine candidates for experimental in vivo and in vitro studies.

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Antibiotics Sensitivity Profile Towards <i>Staphylococcus hominis</i> in Assir Region of Saudi Arabia

Cited by 3 publications

References 14 publications

Antibiotic resistance and mecA characterization of Staphylococcus hominis from filarial lymphedema patients in the Ahanta West District, Ghana: A cross‐sectional study

Antibiotic resistance and mecA characterization of Staphylococcus hominis from filarial lymphedema patients in the Ahanta West District, Ghana: A cross‐sectional study

Isolation and Identification of Bacteria Associated with Pre and Post Processing of Groundnut Cake in Sokoto State, North-Western Nigeria

Genome-Based Multi-Antigenic Epitopes Vaccine Construct Designing against Staphylococcus hominis Using Reverse Vaccinology and Biophysical Approaches

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