Antibiotics Susceptibility Pattern of Bacteria Isolated from Ready-To-Eat Vegetables Sold in Mohammadu Gumi Market, Kaduna, Nigeria

Abstract

Background: Freshly consumed vegetables are considered to have more nutritional value than cooked ones. However, they are a potential source of foodborne illnesses due to possible microbial contamination, this poses safety threat.Objectives: This study was carried out to determine the bacteriological quality of ready-to-eat vegetables sold in Mohammadu Gumi market, Kaduna and to determine the antibiotics resistance pattern of the bacteria isolates.Methods: A total of 40 samples of cabbage were collected. Gram staining, growth on selective media and biochemical tests were carried out to identify the isolates. Antimicrobial susceptibility testing was done using the Kirby-Bauer agar disc diffusion method. Methicillin resistant Staphylococcus aureus isolates were detected with the use of cefoxitin disc agar diffusion test.Results: A total of 46 bacteria isolates were obtained with a total colony count range from 4 – 9 x 106CFU/ml. The following bacteria were isolated: aureus (41.3%), Escherichia coli (28.3%), Salmonella spp (19.6%), Pseudomonas aeruginosa (8.7%) and Serratia spp (2.2%). The result of antimicrobial susceptibility test showed thatthe isolates were highly susceptible to ofloxacin: Staphylococcus aureus, Pseudomonas aeruginosa, Serratia spp (100%), E. coli (92.3%) and Salmonella spp (87.5%). The isolates were all resistant to penicillin and ampicillin while 89.5% of the Staphylococcus aureus isolates were phenotypically methicillin resistant Staphylococcus aureus.Conclusion: This study showed that the vegetables (cabbage) were highly contaminated with antibiotic resistant bacteria, this can be a source of infection to the consumers and a potential means of transmitting multidrug resistant bacteria strains in the community.

Extended Spectrum Beta-Lactamase (ESBL) Resistant genes in Salmonella species Isolated from Ready-to-eat Foods and Food Handlers in Port Harcourt Metropolis, Nigeria

“…Only 1 isolate harboured all three genes namely CTX-M, TEM and SHV. Data obtained from this study correlate with report from other researchers Obajuluwa et al [36] who showed that ESBLs genes may vary from source of infection to another and from one location to another. Other studies have also implicated other bacterial isolates such as Proteus mirabilis as reservoirs of these resistance genes.…”

Isolation and identification of bacteria from exposed and sliced Allium cepa (onions)

“…The suspected bacterial isolates were ten (10) Coagulase Negative Streptococci (63%), four (4) Staphylococcus aureus (25%), one (1) Streptococcus pneumoniae (6%), and one (1) Streptococcus pyogenes (6%). In a related study by Obajuluwa et al 23 more gram negative organisms were isolated, out of a total of 46 bacteria isolates which disclosed the following bacterial isolate: Staphyloccocus aureus (41.3%), Escherichia coli (28.3%), Salmonella spp (19.6%), Pseudomonas aeruginosa (8.7%) and Serratia spp (2.2%). According to Kayode et al 24 gram positive bacteria isolates from selected vegetables were similar to this present study where all isolates were gram positive; and the bacterial isolates from cabbage include; Brevibacillus laterosporus, and Lactobacillus sp.…”