Antibiotics Susceptibility Patterns and Plasmid Profile of Staphylococcus aureus Isolated from Patients with Wound Infections Attending Four Hospitals in Akure, Ondo State

Oluyele, O; Oladunmoye, M. K.

doi:10.9734/jamb/2017/33879

Cited by 3 publications

(3 citation statements)

References 10 publications

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“…Legend: Aug= augmentin, Gen= gentamicin, Cot= cotrimoxazole, Nit= nitrofurantoin, Cro= Ceftriaxone The presence of multiple plasmids observed in some of the isolates in this study could probably be responsible for the resistance observed. Plasmids have been shown to carry genes for antibiotics resistance, a single plasmid can carry genes for resistance to multiple antibiotics [24]. This is in coherence with the work of [16] who also reported multiple plasmids in E. coli O157 isolates from water and fecal samples.…”

Section: Fig 3 Mean Diameter Of Zones Of Inhibition (Mm) Of Commercsupporting

confidence: 73%

Antibiotics Susceptibility Pattern and Plasmid Profile of Escherichia coli O157 Isolated from Fecal Samples of Apparently Healthy Children

Falowo

Oluyele

Oladunmoye

2018

IJPR

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Escherichia coli O157:H7 is an enterohemorrhagic serotype of the bacterium Escherichia coli. It is a cause of severe colitis, bloody diarrhoea and Hemolytic uremic syndrome (HUS) which is associated with life threatening systemic manifestations. This study was designed to investigate the occurrence, antibiotics susceptibility pattern and plasmid profile of E. coli O157 from fecal samples of children. A total of 311 fecal samples were collected from apparently healthy children (111 males and 200 females) within the age range of 0-5 years at Mother and Child Hospital, Anchorite Daycare, Women's' Forum Daycare and FUTA Daycare in Akure, Ondo State, Nigeria. The fecal samples were screened using standard bacteriological and serological techniques. Twenty one (6.75%) of the samples were positive for E. coli O157. Antibiotics susceptibility testing was carried out using disk diffusion technique, many of the isolates displayed multi-drug resistance to the antibiotics employed in this study. The resistance pattern observed is as follows: Ceftriazone 19 (90.5%), Amoxycilin 18 (85.7%), Tetracycline 13 (61.9%), Gentamycin 11(52.4%), Cotrimoxazole 9 (42.9%), Augmentin 8 (25.8%), Ofloxacin 2 (9.5%), Ciprofloxacin 2 (9.5%) and Nitrofurantoin 2 (9.5%). Plasmid profiling of the strains revealed the presence of multiple plasmids Falowo et al.; IJPR, 1(1): 1-9, 2018; Article no.IJPR.41237 2 ranging in sizes from 4.0 to 17.5 kb. Conclusively, since the mode of transmission of this pathogen is via the fecal-oral route and an exceptionally low dose of the organism is able to cause infection. It is therefore recommended that routine screening is carried out to determine the carrier rate of the organism, more public awareness should be carried out to educate the community on need for adherence to personal hygiene, environmental hygiene and good food handling practices to prevent an outbreak. Original Research Article

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Section: Fig 3 Mean Diameter Of Zones Of Inhibition (Mm) Of Commercsupporting

confidence: 73%

Antibiotics Susceptibility Pattern and Plasmid Profile of Escherichia coli O157 Isolated from Fecal Samples of Apparently Healthy Children

Falowo

Oluyele

Oladunmoye

2018

IJPR

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“…Last, the distinct structural environment of H. francisci's dermal denticles, characterized by pronounced riblets and troughs, may be less hospitable to Staphylococcus, despite the potential assistance provided by mucus produced from a more exposed epidermal environment. Studies outline Staphylococcus species, such as S. aureus, to be considered common primary pathogens and implicated in wound infections [48][49][50] .…”

Section: Discussionmentioning

confidence: 99%

Elasmobranchs Exhibit Species-Specific Epidermal Microbiomes Guided by Denticle Topography

Goodman,

Papudeshi,

Mora

et al. 2024

Preprint

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Elasmobranch epidermal microbiomes are species-specific, yet microbial assembly and retainment drivers are mainly unknown. The contribution of host-derived factors in recruiting an associated microbiome is essential for understanding host-microbe interactions. Here, we focus on the physical aspect of the host skin in structuring microbial communities. Each species of elasmobranch exhibits unique denticle morphology, and we investigate whether microbial communities and functional pathways are correlated with the morphological features or follow the phylogeny of the three species. We extracted and sequenced the DNA from the epidermal microbial communities of three captive shark species: Horn (Heterodontus francisci), Leopard (Triakis semifasciata), and Swell shark (Cephaloscyllium ventriosum) and use electron microscopy to measure the dermal denticle features of each species. Our results outline species-specific microbial communities, as microbiome compositions vary at the phyla level;C. ventriosumhosted a higher relative abundance of Pseudomonadota and Bacillota, whileH. francisciwere associated with a higher prevalence of Euryarchaeota and Aquificae, and Bacteroidota and Crenarchaeota were ubiquitous withT. semifasciata. Functional pathways performed by each species' respective microbiome were species-specific metabolic. Microbial genes associated with aminosugars and electron-accepting reactions were correlated with the distance between dermal denticles, whereas desiccation stress genes were only present when the dermal denticle overlapped. Microbial genes associated with Pyrimidines, chemotaxis and virulence followed the shark phylogeny. Microbial genera display associations that resemble host evolutionary lineage, while others had linear relationships with interdenticle distance. Therefore, denticle morphology was a selective influence for some microbes and functions in the microbiome contributing to the phylosymbiosis.

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“…The distinct, well-isolated colonies were then studied for their cultural and morphological characteristics. Gram staining and biochemical tests were conducted to confirm the identification of the isolates [11].…”

Section: Isolation and Identification Methodsmentioning

confidence: 99%

Susceptibility Patterns of Multiple Antibiotic-Resistant Bacteria from Wound and Urine Samples to the Extract ofSpondias Mombin(Linn)

Okiti,

Oladunmoye,

Ogundare

2024

Preprint

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This study evaluates the antibacterial activity ofSpondias mombinL. against multiple antibiotic-resistant bacteria isolated from wound and urine samples of patients attending five (5) selected hospitals in Akure, Ondo State, Nigeria. A total of 313 bacterial isolates were isolated from 353 samples of wound and urine using standard bacteriological procedures, withPseudomonas aeruginosaandStaphylococcus aureusbeing the most predominant in urine and wound samples, respectively. The methanolic extract ofS. mombinwas the most effective against wound isolates, while the aqueous extract was the most effective against urine isolates. The results showed that the methanol extract ofS. mombinhad a zone of inhibition of 24.00±0.00, 30.67±0.33 and 19.33±0.33 mm respectively, againstS. aureus, S. epidermidisandP. aeruginosaat 100 mg/ml. The aqueous extract had a zone of inhibition of 24.67±0.33, 27.33±0.33, 18.67±0.33, 24.67±0.33, 23.67±0.33, 21.33±0.33 and 21.67±0.33 mm againstEscherichia coli, Klebsiella pneumoniae, P. aeruginosa, Proteus mirabilis, S. aureus, S. saprophyticusandTrichomonas vaginalisrespectively, at 100 mg/ml. The phytochemical constituents of the extracts include alkaloids, anthraquinones, cardiac glycosides, flavonoids, phenols, saponins, steroids and tannins. These compounds may be responsible for the antibacterial activity ofS. mombinagainst the multiple antibiotic-resistant bacterial isolates. The findings of this study demonstrates the potential ofS. mombinas an alternative treatment for multiple antibiotic-resistant bacteria from wound and urine.

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Antibiotics Susceptibility Patterns and Plasmid Profile of Staphylococcus aureus Isolated from Patients with Wound Infections Attending Four Hospitals in Akure, Ondo State

Cited by 3 publications

References 10 publications

Antibiotics Susceptibility Pattern and Plasmid Profile of Escherichia coli O157 Isolated from Fecal Samples of Apparently Healthy Children

Antibiotics Susceptibility Pattern and Plasmid Profile of Escherichia coli O157 Isolated from Fecal Samples of Apparently Healthy Children

Elasmobranchs Exhibit Species-Specific Epidermal Microbiomes Guided by Denticle Topography

Susceptibility Patterns of Multiple Antibiotic-Resistant Bacteria from Wound and Urine Samples to the Extract ofSpondias Mombin(Linn)

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