1985
DOI: 10.1016/0092-8674(85)90210-7
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Antibodies against a synthetic peptide as a probe for the kinase activity of the avian EGF receptor and v-erbb protein

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1985
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Cited by 290 publications
(125 citation statements)
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“…The transforming protein produced from the v-erb-B oncogene is a doubly truncated chicken EGF receptor which lacks virtually the entire extracellular ligand binding domain as well as the most Cterminal autophosphorylation site (Downward et al, 1984a,b;). It has been suggested that the loss of the ligand binding domain of the receptor may leave the tyrosine kinase of v-erb-B in a constitutively active form (Gilmore et al, 1985;Kris et al, 1985). Since the EGF receptor kinase catalyses the transfer of phosphate groups to tyrosine residues of substrate proteins, it would seem likely that the amount of tyrosine kinase activity would be important in influencing the growth rate of cells.…”
mentioning
confidence: 99%
“…The transforming protein produced from the v-erb-B oncogene is a doubly truncated chicken EGF receptor which lacks virtually the entire extracellular ligand binding domain as well as the most Cterminal autophosphorylation site (Downward et al, 1984a,b;). It has been suggested that the loss of the ligand binding domain of the receptor may leave the tyrosine kinase of v-erb-B in a constitutively active form (Gilmore et al, 1985;Kris et al, 1985). Since the EGF receptor kinase catalyses the transfer of phosphate groups to tyrosine residues of substrate proteins, it would seem likely that the amount of tyrosine kinase activity would be important in influencing the growth rate of cells.…”
mentioning
confidence: 99%
“…To date, nine retroviral oncogene products (v-src, v-yes, vfgr, v-fps/fes, v-abl, v-ros, v-erbB, v-fms and v-sea) have been shown to exhibit protein-tyrosine kinase (PTK) activity (Hayman et al, 1985;Hunter & Cooper, 1985;Kris et al, 1985;Barbacid et al, 1981). The amino acid sequence responsible for PTK activity (kinase domain was initially identified in the v-src protein (Collett et al, 1980;Hunter & Sefton, 1980;Levinson et al, 1980) and has been shown to be conserved in the other eight oncogene-encoded proteins (Hunter, 1985).…”
mentioning
confidence: 99%
“…No other major bands were detectable, indicating that this association was specific. Moreover, when another major membrane protein is precipitated [e.g., the epidermal growth factor (EGF) receptor; =2 x 106 receptors per hepatocyte] by an antibody raised against a synthetic peptide corresponding to a fragment of the EGF receptor (23), no insulin receptors were precipitated together with EGF receptors (170 kDa) (lane D). In addition, the coprecipitation ofinsulin receptors with H-2 antigens was not affected by a variety of sugars that could have competed for this interaction if it was only due to the glycoprotein nature of the two proteins (data not shown).…”
mentioning
confidence: 99%