Antibodies against Non-Immunizing Antigens Derived from a Large Immune scFv Library

Moon, Sung Ah; Ki, Min Kyung; Lee, Sungyoung; Hong, Mi Lim; Kim, Misook; Kim, Sungsub; Chung, Junho; Rhee, Sue Goo; Shim, Hyunbo

doi:10.1007/s10059-011-2268-8

Cited by 24 publications

(20 citation statements)

References 20 publications

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“…The first rabbit antibody library selected by phage display was reported by Ridder et al , 143 using a scFv format as in subsequent independent studies. 39, 40, 144, 145, 146, 147, 148, 149, 150, 151 Rabbit antibody libraries in Fab format followed in short succession. 152, 153 Due to the higher expression levels of human compared to rabbit constant domains in bacteria, a chimeric rabbit/human Fab format consisting of rabbit variable domains VL and VH recombinantly fused to human constant domains CL and CH1, respectively, proved particularly successful for the selection of rabbit mAbs by phage display 19, 154, 155, 156, 157, 158, 159, 160 and their subsequent humanization (Figure 5).…”

Section: Generation Of Rabbit Mabsmentioning

confidence: 99%

From rabbit antibody repertoires to rabbit monoclonal antibodies

2017

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In this review, we explain why and how rabbit monoclonal antibodies have become outstanding reagents for laboratory research and increasingly for diagnostic and therapeutic applications. Starting with the unique ontogeny of rabbit B cells that affords highly distinctive antibody repertoires rich in in vivo pruned binders of high diversity, affinity and specificity, we describe the generation of rabbit monoclonal antibodies by hybridoma technology, phage display and alternative methods, along with an account of successful humanization strategies.

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Section: Generation Of Rabbit Mabsmentioning

confidence: 99%

From rabbit antibody repertoires to rabbit monoclonal antibodies

2017

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“…Naïve antibody libraries are constructed using B cells of unimmunized or healthy donors, normally focusing on the IgM isotype. Naïve libraries are supposedly able to be used for the isolation of mAbs against any antigen [96]. The process involved in the generation of naïve antibody libraries is well established and has been described in numerous publications [97][98][99][100].…”

Section: Construction Of Naïve Antibody Library For Phage Displaymentioning

confidence: 99%

“…However, antibodies isolated from naïve antibody libraries usually exhibit lower affinities compared to antibodies isolated from immunized libraries. This is because the naïve repertoire would not have undergone in vivo affinity maturation to produce higher affinity antibodies [96]. During antibody library generation, several critical aspects that are monitored includes library size and repertoire diversity in determining the quality of the library [102].…”

Section: Construction Of Naïve Antibody Library For Phage Displaymentioning

confidence: 99%

Naïve Human Antibody Libraries for Infectious Diseases

Chan

Rahumatullah

Lai

et al. 2017

Advances in Experimental Medicine and Biology

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Many countries are facing an uphill battle in combating the spread of infectious diseases. The constant evolution of microorganisms magnifies the problem as it facilitates the re-emergence of old infectious diseases as well as promote the introduction of new and more deadly variants. Evidently, infectious diseases have contributed to an alarming rate of mortality worldwide making it a growing concern. Historically, antibodies have been used successfully to prevent and treat infectious diseases since the nineteenth century using antisera collected from immunized animals. The inherent ability of antibodies to trigger effector mechanisms aids the immune system to fight off pathogens that invades the host. Immune libraries have always been an important source of antibodies for infectious diseases due to the skewed repertoire generated post infection. Even so, the role and ability of naïve antibody libraries should not be underestimated. The naïve repertoire has its own unique advantages in generating antibodies against target antigens. This chapter will highlight the concept, advantages and application of human naïve libraries as a source to isolate antibodies against infectious disease target antigens.

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“…Thus, it is easier to obtain high affinity binders specific to an antigen from immune libraries in comparison to naïve libraries due to the biased nature of the repertoire post-exposure of the antigen. The size of an immune library need not be as large as naïve libraries per se, but it can also be applied for other targets but may not be suitable for self-antigens [56,62]. The obvious limitation of an immune library is the possibility of generating immune libraries of human donors against various targets.…”

Section: Immune Antibody Librariesmentioning

confidence: 99%

“…The biased nature of the library repertoire also means that the library is mainly useful against the antigen used for immunization. Therefore new libraries are required when dealing with targets of different diseases [62]. However, it is also possible for immune libraries to successfully enrich antibodies against non-related targets of the disease of origin.…”

Section: Immune Antibody Librariesmentioning

confidence: 99%