Antibodies enhance CXCL10 production during RSV infection of infant and adult immune cells

Vissers, Marloes; Schreurs, Inge; Jans, Jop; Heldens, Jacco; Groot, Ronald de; Jonge, Marien I. de; Ferwerda, Gerben

doi:10.1016/j.cyto.2015.07.024

Cited by 11 publications

(9 citation statements)

References 42 publications

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“…Although AMs suppress the migration (65) and antigen-presentation capacity (66) of DCs and increase the trafficking of antigen to draining lymph nodes (65), our results show that AM depletion in CD169-DTR mice does not affect DCs or their subset frequency and has no effect on the adaptive CD4 + and CD8 + T cell immune responses to RSV infection. CXCL9 and CXCL10 play important roles in RSV-infected mice by recruiting virus-specific T cells to the lungs and promoting viral clearance (39, 67). Multiple cell populations, including epithelial cells and fibroblasts, may induce the production of CXCL9 and CXCL10 in the airways (68).…”

Section: Discussionmentioning

confidence: 99%

Transient Depletion of CD169+ Cells Contributes to Impaired Early Protection and Effector CD8+ T Cell Recruitment against Mucosal Respiratory Syncytial Virus Infection

Jung

et al. 2017

Front. Immunol.

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Respiratory syncytial virus (RSV) is a major cause of respiratory viral infections in infants and children. Alveolar macrophages (AMs) play a crucial role in combatting airborne pathogens, strongly express CD169, and are localized in the lung alveoli. Therefore, we used CD169-diphtheria toxin receptor (DTR) transgenic mice to explore the roles of CD169+ cells in immune responses to mucosal RSV infection. The administration of diphtheria toxin to CD169-DTR mice induced specific AM depletion and reduced the recruitment of Ly6Chi monocytes. Notably, CD169+ cell depletion reduced levels of innate cytokines, such as interferon-β, IL-6, and TNF-α, in bronchoalveolar lavage fluid during RSV infection without affecting the production of proinflammatory chemokines. Moreover, the depletion of CD169+ cells increased the recruitment of inflammatory cells to the lung during the early stage of RSV infection, although not during the later stages of RSV infection. Furthermore, the depletion of CD169+ cells reduced the recruitment of effector CD8+ T cells to the lungs after RSV mucosal infection. Our findings suggest that modulating the number of CD169+ cells to enhance immune responses to RSV infection may be useful as a new therapeutic strategy.

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Section: Discussionmentioning

confidence: 99%

Transient Depletion of CD169+ Cells Contributes to Impaired Early Protection and Effector CD8+ T Cell Recruitment against Mucosal Respiratory Syncytial Virus Infection

Jung

et al. 2017

Front. Immunol.

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“…Ìn addition, we previously published a microarray database regarding PBMCs from healthy adults that are untreated or stimulated with RSV. The methods have been described by our group previously 35 and this microarray data has been deposited in the NCBI Gene Expression Omnibus (GEO) database under GEO Series accession number (GSE59391) 35. We combined these two microarray databases for the current study.…”

Section: Methodsmentioning

confidence: 99%

“…A similar cut‐off value of 4‐fold difference was used for the microarray data published by Vissers et al. 35 to identify genes that are upregulated In vitro upon exposure of adult PBMCs to RSV. We then determined which genes were upregulated in both microarray analyses, being upregulated genes derived from whole blood from RSV‐infected infants and upregulated genes derived from In vitro RSV‐stimulated adult PBMCs, to identify clinically relevant genes that could be investigated in our model of PBMCs.…”

Section: Methodsmentioning

confidence: 99%

Siglec‐1 inhibits RSV‐induced interferon gamma production by adult T cells in contrast to newborn T cells

et al. 2018

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Interferon gamma (IFN‐γ) plays an important role in the antiviral immune response during respiratory syncytial virus (RSV) infections. Monocytes and T cells are recruited to the site of RSV infection, but it is unclear whether cell‐cell interactions between monocytes and T cells regulate IFN‐γ production. In this study, micro‐array data identified the upregulation of sialic acid‐binding immunoglobulin‐type lectin 1 (Siglec‐1) in human RSV‐infected infants. In vitro, RSV increased expression of Siglec‐1 on healthy newborn and adult monocytes. RSV‐induced Siglec‐1 on monocytes inhibited IFN‐γ production by adult CD4+ T cells. In contrast, IFN‐γ production by RSV in newborns was not affected by Siglec‐1. The ligand for Siglec‐1, CD43, is highly expressed on adult CD4+ T cells compared to newborns. Our data show that Siglec‐1 reduces IFN‐γ release by adult T cells possibly by binding to the highly expressed CD43. The Siglec‐1‐dependent inhibition of IFN‐γ in adults and the low expression of CD43 on newborn T cells provides a better understanding of the immune response against RSV in early life and adulthood.

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“…Vaccine-enhanced disease was observed in a 1960s clinical trial that used formalin-inactivated RSV (FI-RSV)-immunized infants, in whom poorly neutralizing antibodies could form immune complexes with RSV and result in enhanced diseases (Delgado et al, 2009;Polack et al, 2002). However, palivizumab (brand name Synagis ® ), which is a neutralizing antibody that targets F protein of RSV, was an exception to this observation and has been used to protect children at high risk for severe lung disease from RSV (Vissers et al, 2015;Weltzin and Monath, 1999).…”

Section: Introductionmentioning

confidence: 94%

Immunogenicity of an adeno-vector vaccine expressing the F protein of a respiratory syncytial virus manufactured from serum-free suspension culture

Shao

Hsu

et al. 2016

Antiviral Research

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Antibodies enhance CXCL10 production during RSV infection of infant and adult immune cells

Cited by 11 publications

References 42 publications

Transient Depletion of CD169+ Cells Contributes to Impaired Early Protection and Effector CD8+ T Cell Recruitment against Mucosal Respiratory Syncytial Virus Infection

Transient Depletion of CD169+ Cells Contributes to Impaired Early Protection and Effector CD8+ T Cell Recruitment against Mucosal Respiratory Syncytial Virus Infection

Siglec‐1 inhibits RSV‐induced interferon gamma production by adult T cells in contrast to newborn T cells

Immunogenicity of an adeno-vector vaccine expressing the F protein of a respiratory syncytial virus manufactured from serum-free suspension culture

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