Antibodies from lymphocytes used as diagnostic markers: a novel approach

Haaheim, Lars R.; Ibsen, Margrethe W; Skogstrand, Monica

doi:10.1016/s0531-5131(01)00339-9

Cited by 2 publications

(4 citation statements)

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“…The phenomenon of transient antibody formation has been reported previously in the context of influenza and hepatitis B vaccination research (14,22). In support of transient B-cell immune responses to HIV exposure, Tenenbaum et al showed transient anti-HIV-1 seroreactivity in several hemophiliac patients after exposure to HIV-contaminated clotting factor concentrates (28).…”

Section: Discussionmentioning

confidence: 63%

“…In this study, we utilized the PlasmAcute technology to detect anti-HIV-1 antibodies in B-cell lysates before they are detected in serum. This technology is based on the observation that B cells from peripheral blood contain functional antibodies elicited by an infectious agent or vaccine, and these antibodies can be measured before they can be detected in plasma (14,22). This allows a narrowing of the "window period" between immunological stimulation and seroconversion.…”

mentioning

confidence: 99%

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Early Detection of Human Immunodeficiency Virus Type 1-Specific B-Lymphocyte-Derived Antibodies in a High-Risk Population

Odinsen

Parker

Radebe

et al. 2009

Clin Vaccine Immunol

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Diagnosis of acute human immunodeficiency virus (HIV) infection, a key driver of the HIV epidemic, remains a public health challenge. The PlasmAcute technology offers an opportunity to detect early anti-HIV antibody responses. B lymphocytes (B cells) were isolated from the blood of seronegative miners in South Africa by using the PlasmAcute method. B-cell lysates and paired sera were tested for anti-HIV-1 antibodies by two different enzyme-linked immunosorbent assays; immunoreactivity was confirmed by Western blotting. All volunteers were tested for HIV type 1 (HIV-1) viral load, p24 antigen, and CD4 count. Sera from HIV-seronegative men who had positive viral loads and were positive for p24 antigen were retested for anti-HIV antibodies after immune complex dissociation. Anti-HIV antibodies were detected in lysates from 16/259 subjects without immunoreactivity in paired sera. Four subjects, one of whom had a positive viral load initially, subsequently seroconverted. Six subjects showed transient anti-HIV-1 antibodies in the lysates and tested negative for all markers at the follow-up. Five subjects without follow-up data initially had lysate-positive/ serum-negative samples, and these cases were classified as inconclusive. One subject had lysate antibodies and a detectable viral load but was seronegative at follow-up. In conclusion, lysate-derived anti-HIV-1 B-cell antibodies can be detected prior to seroconversion and earlier than or contemporary with HIV-1 RNA detection.

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Section: Discussionmentioning

confidence: 63%

mentioning

confidence: 99%

Early Detection of Human Immunodeficiency Virus Type 1-Specific B-Lymphocyte-Derived Antibodies in a High-Risk Population

Odinsen

Parker

Radebe

et al. 2009

Clin Vaccine Immunol

View full text Add to dashboard Cite

show abstract

“…The enzyme-linked immunospot assay is a well-established method for the study of the immune response and the secretion of antibodies from B lymphocytes in both natural infections and vaccine experiments (5,6,9,11,12), although the procedures are laborious and include time-consuming incubation steps. Further development based on this technology has revealed that specific antibodies can be detected after purified lymphocytes were placed in appropriately coated enzymelinked immunosorbent assay (ELISA) wells and kept for 1 to 2 h at 37°C to allow the spontaneous secretion of antibodies, referred to here as the PlasmAcute technology (9).…”

mentioning

confidence: 99%

“…Further development based on this technology has revealed that specific antibodies can be detected after purified lymphocytes were placed in appropriately coated enzymelinked immunosorbent assay (ELISA) wells and kept for 1 to 2 h at 37°C to allow the spontaneous secretion of antibodies, referred to here as the PlasmAcute technology (9). It has since been discovered that after the separation of the B cells from plasma and other blood components, disruption of the B cells will, surprisingly, directly release functional antibodies that can be measured in immunoassays (11). In clinical studies performed in South Africa, human immunodeficiency virus-specific antibodies were detected in B cells before PCR and before classical seroconversion (12).…”

mentioning

confidence: 99%