Antibodies of patients with Lyme disease to components of the Ixodes dammini spirochete.

Barbour, Alan G.; Burgdorfer, Willy; Grunwaldt, Edgar; Steere, Allen C.

doi:10.1172/jci110998

Cited by 186 publications

(112 citation statements)

References 35 publications

Supporting

Mentioning

107

Contrasting

Order By: Relevance

“…None of the four normal subjects had either IgM or IgG antibodies reactive with B. burgdorferi. In contrast, Barbour et al found crossreactive IgG antibodies in the sera of two of four patients with syphilis, in one instance against a 17-kD antigen and in the other against a 41-kD protein (14). In addition, one of their two patients with relapsing fever had IgG antibodies to a 60-kD polypeptide.…”

Section: Methodsmentioning

confidence: 89%

“…Elevated total IgM levels, which are the best laboratory indicator of disease activity (13), correlate with specific IgM titers, but total IgG levels do not correlate with the specific IgG response (1 1). Using immunoblots, Barbour et al demonstrated spirochetal polypeptides that stimulate the IgG response in serum and joint fluid of patients with Lyme disease (14). In this study, in an effort to better understand the pathogenesis of the arthritis, we defined the sequence in which IgM and IgG antibodies appeared to each of the protein antigens ofB.…”

Section: Introductionmentioning

confidence: 95%

See 1 more Smart Citation

Antigens of Borrelia burgdorferi recognized during Lyme disease. Appearance of a new immunoglobulin M response and expansion of the immunoglobulin G response late in the illness.

Craft¹,

Fischer²,

Shimamoto³

et al. 1986

J. Clin. Invest.

Self Cite

356

174

View full text Add to dashboard Cite

Using immunoblots, we identified proteins ofBorrelia burgdorferi bound by IgM and IgG antibodies during Lyme disease. In 12 patients with early disease alone, both the IgM and IgG responses were restricted primarily to a 41-kD antigen. This limited response disappeared within several months. In contrast, among six patients with prolonged illness, the IgM response to the 41-kD protein sometimes persisted for months to years, and late in the illness during arthritis, a new IgM response sometimes developed to a 34-kD component of the organism. The IgG response in these patients appeared in a characteristic sequential pattern over months to years to as many as 11 spirochetal antigens. The appearance of a new IgM response and the expansion of the IgG response late in the illness, and the lack of such responses in patients with early disease alone, suggest that B. burgdorferi remains alive throughout the illness.

show abstract

Section: Methodsmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 95%

Antigens of Borrelia burgdorferi recognized during Lyme disease. Appearance of a new immunoglobulin M response and expansion of the immunoglobulin G response late in the illness.

Craft¹,

Fischer²,

Shimamoto³

et al. 1986

J. Clin. Invest.

Self Cite

356

174

View full text Add to dashboard Cite

show abstract

“…In Campylobacterjejuni (16) and Salmonella typhi (17) infections patients develop antibodies to flagella during the course of disease. Antibodies to the major flagellar proteins are also common in these spirochetal diseases: syphilis (18), relapsing fever (6,19), and Lyme disease (20)(21)(22). Isolated flagella of B. burgdorferi have been used as the basis of an enzyme-linked immunoabsorbent assay (ELISA) for Lyme disease (23,24).…”

Section: Introductionmentioning

confidence: 99%

A flagella-less mutant of Borrelia burgdorferi. Structural, molecular, and in vitro functional characterization.

Sadziene

Thomas²,

Bundoc³

et al. 1991

J. Clin. Invest.

Self Cite

View full text Add to dashboard Cite

A nonmotile mutant of Borrelia burgdorferi, the etiologic agent of Lyme disease, was isolated and characterized. The mutant was compared with the wild-type predecessor as well as with a motile back-revertant of the same genetic background. The mutant lacked, by morphologic, biochemical, and immunologic criteria, the major structural protein of flagella, flagellin. This mutation was not associated with major DNA rearrangements or with failure of transcription. An apparent consequence of a loss of flagella was reduced ability to penetrate human endothelial cell layers in vitro. In another assessment of functional significance, the flagella-less mutant was equal if not superior to flagella-bearing, isogenic isolates when examined in an enzyme-linked immunosorbent assay for anti-B. burgdorferi antibodies in the sera of Lyme disease patients. These studies of a mutant, the first among pathogenic Borrelia spp. to be characterized, indicate that the flagellum and motility it confers play a role in B. burgdorferi's invasion of human tissues. A flagellaless B. burgdorferi may be useful as the basis of a more specific immunoassay and a vaccine for protection against Lyme disease. (J. Clin. Invest. 1991. 88:82-92.)

show abstract

“…We therefore elected to use an adsorption procedure to help reduce nonspecific binding when performing initial screenings for antibodies to B burgdorferi. Selection of an appropriate bacterium for adsorption required that: 1) it not be closely related to B burgdorferi, and 2) it not have protein A-like binding properties similar to StaphyIococcus uurei~s (9,14). Both of these requirements were met with the use of E coli.…”

Section: Discussionmentioning

confidence: 99%

An adsorption procedure to increase the specificity of enzyme‐linked immunosorbent assays for lyme disease without decreasing sensitivity

Fawcett

O'brien

Doughty

1989

Arthritis & Rheumatism

View full text Add to dashboard Cite

show abstract

Antibodies of patients with Lyme disease to components of the Ixodes dammini spirochete.

Cited by 186 publications

References 35 publications

Antigens of Borrelia burgdorferi recognized during Lyme disease. Appearance of a new immunoglobulin M response and expansion of the immunoglobulin G response late in the illness.

Antigens of Borrelia burgdorferi recognized during Lyme disease. Appearance of a new immunoglobulin M response and expansion of the immunoglobulin G response late in the illness.

A flagella-less mutant of Borrelia burgdorferi. Structural, molecular, and in vitro functional characterization.

An adsorption procedure to increase the specificity of enzyme‐linked immunosorbent assays for lyme disease without decreasing sensitivity

Contact Info

Product

Resources

About