Antibodies Reacting with Lipopolysaccharides from Bacteroides melaninogenicus, Bacteroides fragilis, and Fusobacterium nucleatum in Serum from Normal Human Subjects

Hofstad, Tor

doi:10.1093/infdis/129.3.349

Cited by 57 publications

(22 citation statements)

References 18 publications

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“…of the normal bacterial flora (Quick, Goldberg and Sonnenwirth, 1972;Hofstad, 1974;Kasper, 1976). Although the present study did not provide any evidence that these types of antibodies may be involved in the bactericidal action of normal serum and serum from infected patients, it showed that heat-labile components of human serum (Muschel, 1960), and perhaps other factors, are essential for the inhibition of all serum-sensitive Bacteroides spp.…”

Section: Discussioncontrasting

confidence: 68%

The Bactericidal Action of Human Serum on Bacteroides Species

Rotimi

Eke

1984

Journal of Medical Microbiology

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SUMMARY. Sera obtained from 60 healthy normal subjects were tested for their inhibitory action against four clinical isolates of the Bacteroides fragilis group. All the sera inhibited clinical strains of B. vulgatus and B. ovatus, 92% of 60 sera inhibited B. thetaiotaomicron and 62% inhibited B. fragilis. Bacterial inhibition required components of uninactivated serum and was related to the serum concentrations and species of the bacteria. The viable counts of all the strains, except B. fragilis, were significantly reduced in pooled sera from patients with anaerobic systemic infections and from non-infected patients. The bactericidal activity of the sera from infected patients was more pronounced than that of the 'normal' sera, on all strains except B. fragilis. These observations may explain in part the predominance of B. fragilis in serious clinical infections involving anaerobes.

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Section: Discussioncontrasting

confidence: 68%

The Bactericidal Action of Human Serum on Bacteroides Species

Rotimi

Eke

1984

Journal of Medical Microbiology

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“…However, antibodies may also be detected in a large number of healthy persons, if a wide range of serotypes is used. Such antibodies were not detected in normal sera when antigens of only one or three strains of B. fragilis were used (Ross and Gilmore, 1978;Rissing et al, 1979); but other workers have demonstrated them in normal sera (Quick et al, 1972 ;Danielsson et al, 1974 ;Hofstad, 1974).…”

Section: Discussionmentioning

confidence: 99%

“…1986; revised version accepted 6 Nov. 1986. individuals (Danielsson et al, 1974;Hofstad, 1974;Elhag et al, 1986) may cause difficulties in interpretation of the findings. Furthermore, the antigenic heterogeneity of the group (Elhag et al, 1977) and the geographic variation of their serotypes (Elhag and Tabaqchali, 1978b) may hamper the selection of appropriate test antigens.…”

Section: Introductionmentioning

confidence: 99%

Difficulties in the serodiagnosis of infection with the fragilis group of Bacteroides

Elhag

Bahar

Mahajan

1987

Journal of Medical Microbiology

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Summary. Bacteroides antibodies were studied in sera from 74 patients infected with the fragilis group of Bacteroides and 74 healthy control persons, by immunofluorescence of 26 different serotypes of the fragilis group. Antibodies were present at titres of 10-320 in 65 (88%) patients and 50 (68%) controls (p < 0.01). Titres of 2 80 were demonstrated in sera of 38 (51%) patients and 5 (7%) controls (p < 0.01). Specific IgM antibodies were detected in sera of 42 (57%) patients at a geometric mean titre (GMT) of 30, and 8 (1 1%) controls at a GMT of 11 (p <0-01). High antibody titres as well as specific IgM were found in 32 (43%) patients, while none of the controls showed such a combination (p <0.01). The majority of positive patients' sera (57%) reacted with five or more serotypes, whereas most positive control sera (51%) reacted against only one or two serotypes (p < 0.01). A selected combination of serotypes not reacting with the control sera showed positive reactions with 52 (70%) patients' sera. These findings may be useful in devising schemes for the serodiagnosis of infection caused by the fragilis group of Bacteroides. However, there are indications of geographic variation in prevalence of serotypes, which may prevent the development of a single universal scheme.

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“…This would indicate that these organisms may display similar activity in the human complement system without the participation of reactive immunoglobulins and the classical complement pathway. Since it has been shown (Hofstad 1974, Kristoffersen 1969) that human sera contain antibodies reactive with antigenic preparations of Eusobacterium species, it appears that any contribution to the anticomplementary activity by these immunoglobulins could be controlled in assays utilizing the chelator system presented here. Commercial materials and equipment are identified in this report to specify the investigative procedures.…”

Section: Discussionmentioning

confidence: 99%

“…The factors associated with its anticomplementary (AC) activity in guinea pig sera have been iso-lated in the Gram negative cell wall of the organism (Hawley & Falkler 1978). In order der to progress with our investigations of the alternative pathway in human sera, and also to control the classical pathway activity of antibodies in human sera that may react with the Bacteroidaceae (Falkler & Hawley 1975, Hofstad 1974, Kristoffersen 1969, Kristoffersen & Hofstad 1970, it would be necessary to modify the complement consumption assay used previously by employing divalent cation chelators. Fine (1974) and Fine et al (1972) have previously found that ethylamine diamine tetra acetic acid (EDTA) inhibited the AC activity of hemolysin sensitized erythrocytes (EA), zymosan, lipopolysaccharide (LPS), and Escherichia coli in human sera.…”

Section: Introductionmentioning

confidence: 99%

The effect of divalent cation chelators and magnesium on activation of the alternative complement pathway by Fusobacterium polymorphum (nucleatum)

Hawley

Falkler

1979

J of Periodontal Research

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A complement consumption assay was used to investigate the anti‐complementary activity (AC) of Fusobacerium polymorphum (nucleateum) in guinea pig complement (GPC) which had been treated with the divalent cation chelators ethylamine diamine tetra acetic acid (EDTA) or ethylene glycol tetra acetic acid (EGTA). In the presence of 1 mM EGTA, a cell wall preparation of F. polymorphum reduced the hemolytic complement activity of GPC to essentially the same degree as that observed in the non‐chelated serum controls.hemolysin sensitized sheep erythrocytes (EA). A 1 mM concentraction of EDTA inhibited both the AC activity of F. polymorphum cell wall and the lysisi of EA via the classical pathway. It was also shown that the addition of 1 mM magnesium ion(Mg++)reversed the inhibitory effects of 1 mM EDTA on the lysis EA and on the apparent alternative pathway acitivity displayed by F. polymorphum cell walls. In contrast, the same concentration of Mg++ in the 1 mM EGTA tests did not reverse the EGTA inhibition of the classical pathwaym nor did it markedly augment the alternative pathway. These results indicate that the observed AC activity demonstrated by the cell wall preparation of F. polymorphum did not depend upon a functional classical pathway, but that it porbably occurred exclsively via the alternative complement pathway.

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Antibodies Reacting with Lipopolysaccharides from Bacteroides melaninogenicus, Bacteroides fragilis, and Fusobacterium nucleatum in Serum from Normal Human Subjects

Cited by 57 publications

References 18 publications

The Bactericidal Action of Human Serum on Bacteroides Species

The Bactericidal Action of Human Serum on Bacteroides Species

Difficulties in the serodiagnosis of infection with the fragilis group of Bacteroides

The effect of divalent cation chelators and magnesium on activation of the alternative complement pathway by Fusobacterium polymorphum (nucleatum)

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