Antibodies Specific for Modified Nucleosides: An Immunochemical Approach for the Isolation and Characterization of Nucleic Acids

Munns, Theodore W.; Liszewski, M. Kathryn

doi:10.1016/s0079-6603(08)60673-x

Cited by 50 publications

(22 citation statements)

References 173 publications

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“…Sci. USA 83 (1986) the sugar-phosphate backbone as well (6,9,11). This interpretation was supported by the finding that certain monoclonal anti-DNA antibodies could bind to the phospholipid cardiolipin (28), which presents phosphodiesters separated by three carbon atoms, as does the backbone of DNA.…”

Section: Discussionmentioning

confidence: 91%

“…Early studies with lupus sera indicated that oligonucleotides could inhibit the reactions of some antibodies with denatured DNA, in a way that suggested a major role of the purine and pyrimidine bases in the antigenic sites (3,6). Results of affinity chromatography with nucleoside-protein conjugates supported this interpretation (27).…”

Section: Discussionmentioning

confidence: 99%

“…Still other anti-nucleic acid antibodies are found in sera of patients with the autoimmune disease systemic lupus erythematosus (3,6,7,9) and in related diseases in animals (10). Some lupus autoantibodies react with denatured but not native DNA, whereas others react with both forms-usually better with the denatured DNA.…”

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confidence: 99%

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A recognition site on synthetic helical oligonucleotides for monoclonal anti-native DNA autoantibody.

Stollar¹,

Zon²,

Pastor³

1986

Proc. Natl. Acad. Sci. U.S.A.

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The binding site in native DNA for a murine monoclonal anti-DNA autoantibody was investigated by measurements of competitive binding of a series of synthetic helical oligonucleotides. The antibody bound to a (dG-dC)3 or (dGdC)4 core in the center of a base-paired octadecanucleotide. Reactions of analogues containing modifications or substitutions at specific sites indicated that the antibody bound to portions of cytosine and guanine in the major groove, a limited region of the backbone, and the 2-amino group of one guanine in the minor groove. For these interactions to occur, the antibody combining site would straddle the backbone of one of the helical strands of DNA.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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A recognition site on synthetic helical oligonucleotides for monoclonal anti-native DNA autoantibody.

Stollar¹,

Zon²,

Pastor³

1986

Proc. Natl. Acad. Sci. U.S.A.

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“…It should be noted that although methylated nucleosides, including m'A, occur as posttranscriptional modifications of a variety of RNA species in mammalian tissues (Desrosiers et al, 1974;Munns and Liszewski, 1980), there has been no report of endogenous m6A in adrenal medullary tissue. Because of the metabolic pathways available for this chemical, the tissue concentration of m6A is expected to be small.…”

Section: Discussionmentioning

confidence: 99%

Effects of N⁶‐Methyladenosine on the Synthesis of Phenylethanolamine N‐Methyltransferase in Cultured Explants of Rat Adrenal Medulla

Burke

Joh

1982

Journal of Neurochemistry

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Explants of adrenal medullae were cultured in defined media for up to 48 h, during which time the tissue remained histologically intact. Addition of N6-methyladenosine to the medium led to a diminution in the activity of phenylethanolamine N-methyltransferase (EC 2.1.1.28) in the tissue. The enzyme activity was inversely proportional to the concentration N6-methyladenosine in the culture medium. The extent of loss of phenylethanolamine N-methyltransferase, as measured by immunochemical titration, corresponded to the degree of loss in enzyme activity under the same conditions. Furthermore, the decreased amount of enzyme protein was due to a decrease in the rate of synthesis of phenylethanolamine N-methyltransferase. Neither adenosine nor several methylated nucleosides, including 7-methylguanosine, N2-methylguanosine, and 5-methylcytosine, had an effect on the enzyme. Two other adrenal medullary enzymes, monoamine oxidase (EC 1.4.3.4) and acid phosphatase (EC 3.1.3.2), were not affected by addition of N6-methyladenosine to the medium. The results are consistent with the view that this effect of N6-methyladenosine on the concentration of phenylethanolamine N-methyltransferase is due to an inhibition of its biosynthesis rather than to an alteration of its rate of degradation.

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“…The preparation of antibodies directed against methylated nucleosides has been reported [4]. These antibodies allowed retention of nucleosides, cap structures and tRNA on immunoadsorbant columns [S-7] and it has been shown that, in cell-free protein synthesizing systems, antibodies directed against m'Guo can inhibit specifically the in vitro translation of capped mRNA [8,9].…”

Section: Introductionmentioning

confidence: 99%

Inhibition of in vitro translation by antibodies directed against N⁶‐methyladenosine

Dante

Niveleau

1981

FEBS Letters

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Antibodies Specific for Modified Nucleosides: An Immunochemical Approach for the Isolation and Characterization of Nucleic Acids

Cited by 50 publications

References 173 publications

A recognition site on synthetic helical oligonucleotides for monoclonal anti-native DNA autoantibody.

A recognition site on synthetic helical oligonucleotides for monoclonal anti-native DNA autoantibody.

Effects of N⁶‐Methyladenosine on the Synthesis of Phenylethanolamine N‐Methyltransferase in Cultured Explants of Rat Adrenal Medulla

Inhibition of in vitro translation by antibodies directed against N⁶‐methyladenosine

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Antibodies Specific for Modified Nucleosides: An Immunochemical Approach for the Isolation and Characterization of Nucleic Acids

Cited by 50 publications

References 173 publications

A recognition site on synthetic helical oligonucleotides for monoclonal anti-native DNA autoantibody.

A recognition site on synthetic helical oligonucleotides for monoclonal anti-native DNA autoantibody.

Effects of N6‐Methyladenosine on the Synthesis of Phenylethanolamine N‐Methyltransferase in Cultured Explants of Rat Adrenal Medulla

Inhibition of in vitro translation by antibodies directed against N6‐methyladenosine

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About

Effects of N⁶‐Methyladenosine on the Synthesis of Phenylethanolamine N‐Methyltransferase in Cultured Explants of Rat Adrenal Medulla

Inhibition of in vitro translation by antibodies directed against N⁶‐methyladenosine