2003
DOI: 10.1016/s0009-8981(02)00419-9
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Antibodies to centromere antigens measured by an automated enzyme immunoassay

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Cited by 8 publications
(3 citation statements)
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“…On the other hand, like us they found a good correspondence concerning anti-Jo-1-positive immunodiffusion tests vs EIA positivity. González-Buitrago et al recently evaluated anti-centromeric (anti-CENP-B) antibody analysis by EIA and found that it compared well to IF-ANA [27]. In the present study, however, we found that one-third of the EIA-positive anti-centromere-antibodypositive sera were missed by IF microscopy.…”
Section: Discussioncontrasting
confidence: 68%
“…On the other hand, like us they found a good correspondence concerning anti-Jo-1-positive immunodiffusion tests vs EIA positivity. González-Buitrago et al recently evaluated anti-centromeric (anti-CENP-B) antibody analysis by EIA and found that it compared well to IF-ANA [27]. In the present study, however, we found that one-third of the EIA-positive anti-centromere-antibodypositive sera were missed by IF microscopy.…”
Section: Discussioncontrasting
confidence: 68%
“…The centromere proteins CENP-A (17 kDa), CENP-B (80 kDa) and CENP-C (140 kDa) have been identified as the major antigenic targets of ACA in patients with SSc, although sera from a small percentage of ACA positive patients recognize other proteins, such as the 50 kDa CENP-D, the 312 kDa CENP-E, the 400 kDa CENP-F, the 95 kDa CENP-G and the 28 kDa CENP-H [75]. In the last years ELISA [76] is increasingly being used in addition to IIF for the detection of ACA and possibly has a higher sensitivity in some cases [77].…”
Section: Anti-centromere Antibodies: Antibodies Associated With a CLImentioning
confidence: 99%
“…On the other hand, the prevalence of ACA in pSS ranges from 3,7 to 27% [75,76,81e86] when detected by IIF and from 20 to 27% [75,76,87] when detected by other methods, depending on the CENP protein used as a target antigen. It has been recently shown that the pattern of CENP recognition differs significantly between pSS and lcSSc, with the majority of pSS sera recognizing CENP-C alone (70% of pSS vs. 6% of lcSSc) and the majority of lcSSc sera recognizing both CENP-B and CENP-C (83% of lcSSc vs 0% of pSS) [87].…”
Section: Anti-centromere Antibodies: Antibodies Associated With a CLImentioning
confidence: 99%