Antibodies to Oxidized Low-Density Lipoproteins and Angiographically Assessed Coronary Artery Disease in White Patients

Abstract

Background-Low-density lipoprotein (LDL) can be oxidatively modified by reactive oxygen species, thus generating oxLDL. The latter induce formation of specific antibodies (oxLDLAb), which are detectable in patients with atherosclerosis, in which they might play a pathogenic or a protective role. Thus, we aimed to investigate the association of antibodies with oxidized LDLs (oxLDL) (oxLDLAbs) with coronary artery disease (CAD) and acute coronary syndromes. Methods and Results-In a cross-sectional study of 529 consecutive patients undergoing quantitative coronary angiography for suspected CAD, we measured the titer of IgG oxLDLAbs by ELISA. With regression analysis techniques, we also investigated the determinants of oxLDLAb titer and the association of oxLDLAbs with CAD severity. We found no significant differences of oxLDLAb titer between groups of patients without and with different CAD severity. The oxLDLAb titer was 18.6 enzyme units (EU) (11.5 to 25.7 EU/mL) (mean, 95% CI) in patients without CAD; 16.8 EU (9.6 to 24.2 EU) in patients with stenosis Ͻ50%; and 19.9 EU (15 to 24.8 EU), 17.2 (13.8 to 20.6 EU), and 14.7 EU (12.1 to 17.3 EU) in those with in 1-, 2-, or 3-vessel Ն50% stenosis, respectively. Similarly, no differences of oxLDLAb titer between patients without and with acute coronary syndrome were found. The oxLDLAb titer correlated weakly with aging and with serum total, LDL, and HDL cholesterol and plasma homocysteine levels; however, only age and HDL cholesterol remained significant predictors of the oxLDLAb titer at a stepwise regression analysis. Conclusions-The results of this study, which was adequately powered from the statistical standpoint, provided no evidence for an association of IgG oxLDLAb titer with angiographically assessed CAD in whites. (Circulation. 2003; 108:2467-2472.)

Influence of age and sex on levels of anti-oxidized LDL antibodies and anti-LDL immune complexes in the general population

“…In fact, our group found an inverse relation between levels of cholesterol and levels of antioxidized LDL antibodies in the general population (14). Recent studies have found no association between the levels of anti-oxidized LDL antibodies and coronary artery disease (15), and others have detected an inverse relation between IgM autoantibodies to oxidized LDL and carotid artery atherosclerosis (16).…”

Association of anti-oxidized LDL and candidate genes with severity of coronary stenosis in the Women's Ischemia Syndrome Evaluation study

“…In whites, the IgM antibody levels were positively correlated with total ( r 2 = 0.01, P = 0.03) and LDL ( r 2 = 0.017, P = 0.004) cholesterol, and cigarette smoking ( r 2 = 0.014, P = 0.007), and negatively correlated with age ( r 2 = 0.012, P = 0.02). White women with diabetes also had lower IgG antibody levels and atherosclerosis severity by different methods have yielded inconsistent results ( 6,(9)(10)(11)(12) partly because the two antibodies of oxLDL (IgM and IgG) have different mechanistic functions in the atherosclerosis pathway. While IgM antibodies inhibit macrophage uptake of oxLDL ( 13,14 ), oxLDL and IgM immune complexes induce accumulation of macrophages ( 14 ).…”

Relationship of IgG and IgM autoantibodies to oxidized low density lipoprotein with coronary artery disease and cardiovascular events

“…Although most studies have shown a positive association of IgG OxLDL autoantibodies with CAD, particularly smaller angiographic studies (25)(26)(27), others have shown no association (28)(29)(30)(31). Several studies have also shown that IgG OxLDL autoantibodies are increased at baseline in patients with acute myocardial infarction (16,32).…”