Antibodies to Pneumococcal Polysaccharides: Relation between Binding and Electrophoretic Heterogeneity

Abstract

Antibodies to type III and type VIII pneumococcal polysaccharides were examined with respect to ligand binding and electrophoretic heterogeneity. Both antibodies showed apparent binding homogeneity, although multiple light chain and heavy chain electrophoretic species were demonstrated.

Specific Inhibition of Plaque Formation to Phosphorylcholine by Antibody against Antibody

“…The pneumococcal C polysaccharides are, however, excellent antigenic determinants in that large amounts of polysaccharide-specific antibodies are produced in several species immunized with the whole pneumococcal organism (13). The observed PFC response against either the pneumococcal C polysaccharide or phosphorylcholine are almost identical (Tables 1 and 2).…”

Heavy and light chains of a homogeneous immunoglobulin‐G

“…However, for immunoglobulin molecules of limited heterogeneity, a relationship was expected to exist between the number of light and heavy chain bands, and the heterogeneity of the original immunoglobulin preparations. Consequently, the electrophoretic analysis of light and heavy chain sub-units is now employed as a criterion in the search for a homogeneous antibody [3].…”

An Analysis of the Sequences of the Variable Regions of Bence Jones Proteins and Myeloma Light Chains and Their Implications for Antibody Complementarity

“…In some instances, however, relatively homogeneous populations of antibodies with respect to many of these properties have been obtained. Among these have been human antibodies to dextran and levan (8,9) and rabbit antibodies to the group-specific carbohydrate of streptococcus (10-12), antibodies to the Type III-specific capsular polysaccharide of pneumococcus (13,14), rabbit antihapten (15), and specimens of antibodies and of Fab' fragments which crystallized (Nisonoff et al, in references 16,17), but sequence data on these are not yet available.…”