Antibodies to Two Common Pesticides, DDT and Malathion

Centeno, E.R.; Johnson, W.J.; Sehon, A.H.

doi:10.1159/000230215

Cited by 45 publications

(14 citation statements)

References 5 publications

(5 reference statements)

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“…All these methods are important, but immunoassay procedures, including ELISAs, have a number of advantages, including simplicity and cost-effectiveness, which make them particularly useful in routine work. Centeno and Johnson (16) developed rabbit-anti-DDT and anti-malathion antibodies in 1967. Over the past few years, immunoassays have been developed as attractive options for the identification and quantification of a variety of agrochemicals in water, soil, and agricultural products.…”

Section: Discussionmentioning

confidence: 99%

Development and Identification of Monoclonal Antibodies Against Parathion

Zeng

Yang²,

Zhong³

et al. 2005

Hybridoma

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An amino-derivative of parathion was prepared and conjugated to human serum albumin (HSA) and bovine thyroglobulin (BTG) via diazonium condensation. Spleen cells producing high titer antibody were removed and fused with myeloma cells of SP2/0 origin. Using a conventional immunization protocol, we generated nine stable murine monoclonal antibodies (MAbs) producing cell lines to parathion. After four successive limiting dilutions, antibodies produced by nine clones had high affinities, ranging from 10(9) to 10(12) M(-1). These clones were found to be of IgG class and IgM class with k light chain. Subclass determination showed that the clones produced IgG(1), IgG(2a), IgG(2b), and IgM types of antibody. One clone (2H(9)) was used to establish the calibration curve with a sensitivity of 26 ng/mL, a practical working range of 46.8-6000 ng/mL parathion.

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Section: Discussionmentioning

confidence: 99%

Development and Identification of Monoclonal Antibodies Against Parathion

Zeng

Yang²,

Zhong³

et al. 2005

Hybridoma

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“…Repeated exposure to malathion results in allergic responses in man, guinea pigs, rabbits, rats, and mice (Centeno et al, 1970;Cushman and Street, 1983;Hazelton, 1992;Magnusson and Kligman, 1987;Milby and Epstein, 1964;Vijay et al, 1978). Repeated exposure to malathion results in allergic responses in man, guinea pigs, rabbits, rats, and mice (Centeno et al, 1970;Cushman and Street, 1983;Hazelton, 1992;Magnusson and Kligman, 1987;Milby and Epstein, 1964;Vijay et al, 1978).…”

Section: Malathionmentioning

confidence: 99%

Immunotoxicity of Pesticides

Rodgers

2001

Handbook of Pesticide Toxicology

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“…Prior to labeling, target cells were washed two times in HBSS and suspended to 2-20 x 106/ml in media without serum. The cells were labeled by incubating 0.5 ml cell suspension with 100-200 ,uCi 51Cr (0.1-0.2 ml, NEZ-030S, sodium chromate in saline solution, specific activity 200-500 Ci/g, New England Nuclear Corp.) in a 50 ml conical test tube on an aliquot shaker in a humidified atmosphere of 5% CO2 at 37°C for [45][46][47][48][49][50][51][52][53][54][55][56][57][58][59][60] (Fig. 4) …”

Section: Cell-mediated Lymphocytotoxicitymentioning

confidence: 99%

Assessment of environmental contaminant-induced lymphocyte dysfunction.

Silkworth¹,

Loose²

1981

Environ Health Perspect

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Although it has been established that environmental contaminants can alter immune function, the mechanisms of action have yet to be determined. This paper reviews the effects of hydrocarbon environmental contaminants on lymphocyte function and presents an approach which may serve to delineate the mechanisms of action. The approach is based on the use of the developmental phases of an immune response and assays which can be used for their functional assessment. Possible interactions between environmental contaminants and lymphocyte function and factors which must be considered in the evaluation of immune status are discussed. In addition, a study on the influence of the chronic exposure to two polyhalogenated hydrocarbons, PCB and HCB, on several parameters of lymphocyte function in mice is presented.

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Antibodies to Two Common Pesticides, DDT and Malathion

Cited by 45 publications

References 5 publications

Development and Identification of Monoclonal Antibodies Against Parathion

Development and Identification of Monoclonal Antibodies Against Parathion

Immunotoxicity of Pesticides

Assessment of environmental contaminant-induced lymphocyte dysfunction.

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