Antibody as carrier of131I in cancer diagnosis and treatment

Ghose, T.; Guclu, A.; Tai, Joseph; Macdonald, A. S.; Norvell, S. T.; Aquino, J.

doi:10.1002/1097-0142(197511)36:5<1646::aid-cncr2820360518>3.0.co;2-a

Cited by 34 publications

(3 citation statements)

References 11 publications

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“…The specificity of anti-HMEC will allow for the identification of normal and neoplastic breast epithelial cells in mixed primary cultures and in isolated metastases with unknown carcinoma of origin (33), and provide a means for the purification of this cell type from mixed cell suspensions. Also, anti-HMEC could be used as a vector to carry cytotoxic substances to neoplastic breast tissue (33).…”

Section: Resultsmentioning

confidence: 99%

Surface differentiation antigens of human mammary epithelial cells carried on the human milk fat globule.

Ceriani¹,

Thompson²,

Peterson³

et al. 1977

Proc. Natl. Acad. Sci. U.S.A.

212

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Rabbit antibodies against components of the human milk fat globule bind specifically to normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. Variation in indirect immunofluorescence staining in both intensity per cell and percentage of cells stained is observed for the different breast cell lines. Cells derived from other epithelial and other ectodermal tissues, fetal fibroblasts, cells of the blood buffy coat, and even fibroblasts of the breast itself do not bind the antibodies. This suggests that these antibodies are detecting cell-type-specific antigens. These normal breast epithelial cell antigens are on the cell surface and their exptession is stable in long-term cultured cell lines, even after much chromosomal variation in a given line. By affinity chromatography, three distinct antigenic components can be isolated from the milk fat globule, one of which contains carbohydrate. These differentiation antigens of the human breast epithelial cell are not only useful as specific cel-ype markers, but also can provide a tool to study the role of the cell surface in normal and neoplastic mammary development. The production of antibodies directed solely against outer cell surface components is hindered by-the difficulties inherent in purifying such cell membranes. Plasma membrane constituents amount to about 2-3% of the total cell protein (5); thus, separation from other cellular components present in much greater amounts is difficult. Moreover, when a source of normal human tissue, other than blood, is needed for purification of normal cell surface antigens, one is faced with an unresolved problem. Because the milk fat globules are secreted into milk by the breast epithelial cell-by envelopment of fat droplets by the plasma membrane (6)-they represent an accessible source of enriched plasma membrane. Additional information that enzyme markers of the plasma membrane are found in the milk fat globule membrane (7) and the fact that churned milk fat globules appear as an almost pure membrane fraction when examined by electron microscopy (8) make this material the immunogen of choice.Here we describe the production of an anti-human mammary epithelial cell antiserum (anti-HMEC) raised against the defatted human milk fat globule (HMFG), which specifically identifies mammary epithelial cells from both the normal breast and from human breast carcinoma cell lines. Also, we describe characteristics of the antigenic components present on the HMFG, and procedures for their separation. MATERIALS AND METHODSFor the preparation of defatted HMFG, the washed cream fraction of human milk (8) was extracted twice with two volumes of chloroform and twice with 1 volume of ether, and then lyophilized. Electrophoresis was performed in 5% polyacrylamide gels (12 cm long and 0.6 cm in diameter) in the presence of 0.1% sodium dodecyl sulfate, 7 M urea, and 0.1 M sodium phosphate buffer, pH 7.2, with samples dissolved in 1% sodium dodecyl su...

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Section: Resultsmentioning

confidence: 99%

Surface differentiation antigens of human mammary epithelial cells carried on the human milk fat globule.

Ceriani¹,

Thompson²,

Peterson³

et al. 1977

Proc. Natl. Acad. Sci. U.S.A.

212

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“…Several approaches were used as long ago as some 50 years to evaluate the potential therapeutic effect in different xenograft models [2,3]. Promising results were shown in different tumour cell lines in vitro and in vivo [4][5][6][7]. One of the first human applications was reported in the late 1960s [8].…”

mentioning

confidence: 99%

Can radioimmunotherapy promote from an orphan drug to daily clinical practice?

Mottaghy

2014

Eur J Nucl Med Mol Imaging

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“…to sample processing have been described in the literature including: exhaustively perfusing the experimental animals with saline to remove all blood (Koji et al 1980); mincing and homogenizing the tissue and washing repeatedly, blending and weighing the wet specimen (Bernhard et al 1983), washing tissues several times in phosphate-buffered saline "to get rid of as much blood as possible" (Ghose et al 1975); lightly blotting surface blood from the target tissues, weighing and counting them with contained blood present (Wahl and Parker 1983c); carefully removing the tissue, weighing and counting (Hoffer et al 1973); and not clearly specified which we assume to mean simple excision and counting (Primus et al 1973).…”

mentioning

confidence: 99%

The effect of specimen processing on radiolabeled monoclonal antibody biodistribution

1984

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Monoclonal antibodies are assuming increasing importance in experimental and clinical medicine. Generally, tissue biodistribution studies in animals precede human studies. To investigate a concern of ours that varying methods of sample handling in these studies could result in apparent alterations in tissue-binding levels, we compared two methods of tissue processing after the administration of labeled antibodies: one including only blotting away of blood, the other involving several washing steps. The unwashed, blotted specimens were found to have significantly more radioactivity per gram of tissue than the washed, ranging from 22% more in the spleen to 52% more in the lungs and left ventricle. Since in vivo imaging is dependent on the total mount of radioactivity in an organ, we believe the most meaningful determination of tissue radioactivity should be based on unwashed samples. Awareness of this problem is suggested to allow meaningful extrapolations from measured tissue localization data to imaging and therapy.

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Antibody as carrier of131I in cancer diagnosis and treatment

Cited by 34 publications

References 11 publications

Surface differentiation antigens of human mammary epithelial cells carried on the human milk fat globule.

Surface differentiation antigens of human mammary epithelial cells carried on the human milk fat globule.

Can radioimmunotherapy promote from an orphan drug to daily clinical practice?

The effect of specimen processing on radiolabeled monoclonal antibody biodistribution

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