Antibody Avidity and Immunoglobulin G Isotype Distribution following Immunization with a Monovalent Meningococcal B Outer Membrane Vesicle Vaccine

Vermont, Clementien; Dijken, Harry H. van; Limpt, Cees J. P. van; Groot, Ronald de; Alphen, Loek van; Dobbelsteen, Germie P. J. M. van den

doi:10.1128/iai.70.2.584-590.2002

Cited by 86 publications

(91 citation statements)

References 29 publications

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“…Some studies have found no correlation between subclass and serum bactericidal activity (8,14). Others have shown a positive correlation between IgG1 and IgG3 antibodies and serum bactericidal activity in humans (17,38). Interestingly, these subclasses are most effective at complement binding and activation (3).…”

Section: Discussionmentioning

confidence: 99%

Mucosal Vaccination against Serogroup B Meningococci: Induction of Bactericidal Antibodies and Cellular Immunity following Intranasal Immunization with NadA of Neisseria meningitidis and Mutants of Escherichia coli Heat-Labile Enterotoxin

et al. 2004

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Conjugated polysaccharide vaccines protect against serogroup C meningococci. However, this approach cannot be applied to serogroup B, which is still a major cause of meningitis. We evaluated the immunogenicity of three surface-exposed proteins from serogroup B Neisseria meningitidis (App, NhhA, and NadA) identified during whole-genome sequencing. Mice were immunized intranasally with individual proteins in the presence of wild-type Escherichia coli heat-labile enterotoxin (LTwt), LTR72, a partially inactivated mutant, or LTK63, a completely nontoxic mutant, as the adjuvant. Each of the meningococcal proteins induced significant cellular responses; NhhA and NadA induced strong antibody responses, but only NadA induced bactericidal antibody when administered intranasally with mucosal adjuvants. In addition, immunoglobulin A and bactericidal antibodies were detected in the respiratory tract following intranasal delivery of NadA. Analysis of antigenspecific cytokine production by T cells from immunized mice revealed that intranasal immunization with NadA alone failed to generate detectable cellular immune responses. In contrast, LTK63, LTR72, and LTwt significantly augmented NadA-specific gamma interferon, interleukin-4 (IL-4), IL-5, and IL-10 production by spleen and lymph node cells, suggesting that both Th1 and Th2 cells were induced in vivo. The strongest cellular responses and highest bactericidal antibody titers were generated with LTR72 as the adjuvant. These findings demonstrate that the quality and magnitude of the immune responses generated by mucosal vaccines are influenced by the antigen as well as the adjuvant and suggest that nasal delivery of NadA with mucosal adjuvants has considerable potential in the development of a mucosal vaccine against serogroup B meningococci.

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Section: Discussionmentioning

confidence: 99%

Mucosal Vaccination against Serogroup B Meningococci: Induction of Bactericidal Antibodies and Cellular Immunity following Intranasal Immunization with NadA of Neisseria meningitidis and Mutants of Escherichia coli Heat-Labile Enterotoxin

et al. 2004

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“…The expressed gp140 (0.6 g per ml) was captured with sheep anti-gp120 Ab (Cliniqa, San Marcos, CA) at 0.5 g per well in coat buffer (Immunochemistry Technologies, Minneapolis, MN). Avidity assays used parallel enzymelinked immunosorbent assays, one treated with PBS and the other with 1.5 M sodium thiocyanate for 10 min at room temperature (21,38). Avidity indices, or the ratio of the dilution of serum giving an optical density of 0.5 for the sodium thiocyanate wash to the dilution giving an optical density of 0.5 for the PBS wash times 100, showed excellent reproducibility for an in-house reference vaccine serum included in all assays (mean Ϯ standard deviation [SD], 27.3 Ϯ 1.6).…”

Section: Vol 83 2009 High-avidity Anti-env Ab In Heterologous Protementioning

confidence: 99%

Preclinical Studies of Human Immunodeficiency Virus/AIDS Vaccines: Inverse Correlation between Avidity of Anti-Env Antibodies and Peak Postchallenge Viremia

Zhao

Lai

Amara

et al. 2009

J Virol

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“…A monovalent vaccine expresses PorA subtype P1.7-2,4 whereas a hexavalent vaccine contains six highly prevalent serosubtypes. Immunization of children with these vaccines induced a PorA-specific antibody response with a similar IgG isotype distribution as is seen after invasive meningococcal disease (8,9). We and others also showed that vaccination with the monovalent OMV vaccine as well as the hexavalent vaccine induces a significant antibody avidity maturation (9,10), which is a marker of the induction of immunologic memory (11)(12)(13).…”

mentioning

confidence: 69%

“…Antibody avidity was measured using thiocyanate as chaotropic agent (9). In short, Immulon 2 (Dynex Technologies, Inc. Chantilly, USA) 96-well plates were coated overnight with 100 L/well of a monovalent OMV suspension at a concentration of 4 g/mL.…”

Section: Methodsmentioning

confidence: 99%

Porin A—Specific Antibody Avidity in Patients Who Are Convalescing from Meningococcal B Disease

Vermont

Dijken²,

Groot

et al. 2005

Pediatr Res

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Antibody Avidity and Immunoglobulin G Isotype Distribution following Immunization with a Monovalent Meningococcal B Outer Membrane Vesicle Vaccine

Cited by 86 publications

References 29 publications

Mucosal Vaccination against Serogroup B Meningococci: Induction of Bactericidal Antibodies and Cellular Immunity following Intranasal Immunization with NadA of Neisseria meningitidis and Mutants of Escherichia coli Heat-Labile Enterotoxin

Mucosal Vaccination against Serogroup B Meningococci: Induction of Bactericidal Antibodies and Cellular Immunity following Intranasal Immunization with NadA of Neisseria meningitidis and Mutants of Escherichia coli Heat-Labile Enterotoxin

Preclinical Studies of Human Immunodeficiency Virus/AIDS Vaccines: Inverse Correlation between Avidity of Anti-Env Antibodies and Peak Postchallenge Viremia

Porin A—Specific Antibody Avidity in Patients Who Are Convalescing from Meningococcal B Disease

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