Antibody avidity determination by ELISA using thiocyanate elution

Pullen, G.R.; Fitzgerald, M. G.; Hosking, C. S.

doi:10.1016/0022-1759(86)90268-1

Cited by 395 publications

(290 citation statements)

References 13 publications

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“…25 Incubation of serum samples was described above. Plates were washed three times with 0.05% PBS-Tween20, and NaSCN, a chaotropic compound that interferes with the antigenantibody reaction, was added in concentrations ranging from 0.5 to 3.5 M. Plates were left at room temperature for 15 min and then washed six times with PBS-Tween 20.…”

Section: Measurement Of Antibody Aviditymentioning

confidence: 99%

Th2-dominated antitumor immunity induced by DNA immunization with the genes coding for a basal core peptide PDTRP and GM-CSF

et al. 2005

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Our previous study showed that DNA vaccination with a plasmid vector encoding a core peptide of mucin1 (PDTRP) provided modest protection against challenge with tumor cells that expressed mucin1 protein. We report here that a DNA vaccine comprising a modified PDTRP plasmid and GM-CSF coding sequence at the C-terminus induced better protection against tumor challenge. The increased protection was directly correlated with a stronger PDTRP-specific immune response induced by the GM-CSF fusion plasmid. The plasmid encoding GM-CSF and the target PDTRP antigen induced a greater PDTRP-specific Th proliferation, antibodies, and cytotoxicity. Interestingly, the modified plasmid vaccine predominantely enhanced the type 2 immune responses manifested by an increased IgG1 to IgG2a antibody ratio and a greater induction of GATA-3 and IL-4 mRNA than that of T-bet and IFN-g mRNA in spleen cells from vaccinated mice. In addition, protection against tumor challenge in vaccinated mice showed that there was no significant change in mice survival after in vivo CD8 þ CTL depletion, indicating that antitumor immunity augmented by plasmid encoding GM-CSF and target PDTRP gene vaccine was dominated by Th2 immune response.

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Section: Measurement Of Antibody Aviditymentioning

confidence: 99%

Th2-dominated antitumor immunity induced by DNA immunization with the genes coding for a basal core peptide PDTRP and GM-CSF

et al. 2005

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“…6 This IgG anti-rATG was of donor G1 m allotype. The anti-rATG response coincided with a steep decrease of serum rATG (data not shown) and the patient developed severe acute GVHD, grade III at day þ 23.…”

mentioning

confidence: 99%

Antibodies to anti-thymocyte globulin in aplastic anemia patients have a negative impact on hematopoietic SCT

Zijde

Bredius

Jansen-Hoogendijk

et al. 2012

Bone Marrow Transplant

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“…Such methods require large amounts of antibody, and the use of DEA has proved to be a simple and convenient method of assessing relative functional antibody affinities [27]. The use of chaotropic agents in ELISA has been to measure antibody affinity in man and experimental animals to a wide range of antigens [22,23,25,[27][28][29]. The use of chaotropic agents to measure antibody affinity, as with all affinity assays, measures the average affinity of polyclonal antibodies which contain a mixture of both high-and low-affinity antibody.…”

Section: Discussionmentioning

confidence: 99%

“…1). The use ofmildchaotropic agents in ELISA has been shown to have a differential effect on the dose-response curve, producing a marked shift to the left with low-aflinity antibodies, but having less effect on high-affinity antibodies [22,23].…”

Section: Subjectsmentioning

confidence: 99%

Low antibody affinity restricted to the IgA isotype in IgA nephropathy

Layward

Allen

Hattersley

et al. 1994

Clinical and Experimental Immunology

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SUMMARYAntibody affinity affects the handling and behaviour of immune complexes, and experimental studies have shown that animals which produce predominantly low-affinity antibody are prone to immune complex deposition resulting in glomerulonephritis. In order to investigate the potential role of antibody affinity in the pathogenesis of IgA nephropathy, affinity of both IgA and IgG antibody isotypes during secondary response to systemic immunization with tetanus toxoid was studied in 20 patients with IgA nephropathy. Patients with IgA nephropathy produced IgA antibodies of significantly lower affinity than controls {P<000\), whereas IgG antibody affinities were similar. Contrasting with controls, patients' IgA antibody affinity was inversely related to antibody concentration, with higher responders producing large amounts of low-affinity antibody. IgG antibody affinity increased with time, and maturation of IgG antibody affinity was similar in both controls and patients. IgA affinity in controls decreased with time, and this lack of IgA affinity maturation may explain the relative unimportance of IgA in normal systemic immunity. This temporal decrease in IgA affinity was not observed in patients with IgA nephropathy. The production of low-affinity IgA in IgA nephropathy may provide an explanation for the predominant deposition of IgA in this disease.

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Antibody avidity determination by ELISA using thiocyanate elution

Cited by 395 publications

References 13 publications

Th2-dominated antitumor immunity induced by DNA immunization with the genes coding for a basal core peptide PDTRP and GM-CSF

Th2-dominated antitumor immunity induced by DNA immunization with the genes coding for a basal core peptide PDTRP and GM-CSF

Antibodies to anti-thymocyte globulin in aplastic anemia patients have a negative impact on hematopoietic SCT

Low antibody affinity restricted to the IgA isotype in IgA nephropathy

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