Antibody-based donor–acceptor spatial reconfiguration in decorated lanthanide-doped nanoparticle colloids for the quantification of okadaic acid biotoxin

Abstract

With the increasing movement away from the mouse bioassay for the detection of toxins in commercially harvested shellfish, there is a growing demand for the development of new and potentially field-deployable tests in its place. In this direction we report the development of a simple and sensitive nanoparticle-based luminescence technique for the detection of the marine biotoxin okadaic acid. Photoluminescent lanthanide nanoparticles were conjugated with fluorophore-labelled anti-okadaic acid antibodies which, upon binding to okadaic acid, gave rise to luminescence resonance energy transfer from the nanoparticle to the organic fluorophore dye deriving from a reduction in distance between the two. The intensity ratio of the fluorophore : nanoparticle emission peaks was found to correlate with okadaic acid concentration, and the sensor showed a linear response in the 0.37-3.97 μM okadaic acid range with a limit of detection of 0.25 μM. This work may have important implications for the development of new, cheap and versatile biosensors for a range of biomolecules and that are sufficiently simple to be applied in the field or at point-of-care.

Reagents for Mass Cytometry

“…The synthetic aptamer (GGGAGGACGAUGCGGAUCAGCCAUGUUUACGUC-ACUCCU) was labeled with −(CH 2 ) 6 −SH at the 5′-end. The aptamer was coupled to a maleimide-end-capped MCP labeled with 167 Er. While the polymer itself was not identified, it appeared to be a Maxpar polymer and coupling followed Standard Biotools (formerly Fluidigm) protocol.…”

“…To use NPs as reagents in mass cytometry bioassays, the NPs must be conjugated to Abs or to another type of bioaffinity agent. Although the nanomaterials literature is rich with examples of Ab-NP conjugates, − there are unique requirements for employing these materials as reagents in mass cytometry, including colloidal stability in phosphate-containing buffers, and minimal NSB to cells.…”

Binding nature and conformational alternations of bovine serum albumin upon interaction with synthesized LaF3:Ce,Tb luminescent nanocrystals using multi-spectroscopic approach

“…Compared with conventional fluorescent materials such as organic dyes and quantum dots, lanthanide-doped luminescent materials have many advantages in many application fields, such as biological imaging, drug delivery and biosensor [3][4][5][6]. Notwithstanding lanthanide-doped luminescent materials have shown great promise in bioapplications, they may have some potential toxicity on biomolecules.…”