Antibody biosensors for the measurement and characterization of soluble CD147 molecules

Laopajon, Witida; Takheaw, Nuchjira; Khummuang, Saichit; Kampoun, Tanyaluck; Cheunsirikulchai, Kantinan; Kasinrerk, Watchara; Pata, Supansa

doi:10.12932/ap-150217-0022

Cited by 3 publications

(2 citation statements)

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“…The signaling pathways generated by this mAb that are responsible for the negative regulation of T cell activation are of interest for future investigation. In addition, by engaging the mAb MEM-M6/6, which reacts to CD147 Ig-like domain 2 (10,17), our results revealed that the functional epitope recognized by this mAb regulates T cell activation via CD147 expression on T cells and monocytes.…”

Section: Discussionmentioning

confidence: 76%

“…As the anti-CD147 mAbs M6-1E9, M6-1D4 and MEM-M6/6 inhibited T cell proliferation, to confirm that the inhibitory effect was caused by binding of the mAbs to its epitope on the CD147 molecule, a neutralization assay was performed using the recombinant CD147 molecule CD147-IgG ( 10 , 17 ). The binding ability of mAbs M6-1E9, M6-1D4 and MEM-M6/6 to CD147-IgG was first determined by indirect ELISA and found that all mAbs reacted to CD147-IgG (data not shown).…”

Section: Resultsmentioning

confidence: 99%

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Differential CD147 Functional Epitopes on Distinct Leukocyte Subsets

et al. 2021

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CD147, a member of the immunoglobulin (Ig) superfamily, is widely expressed in several cell types. CD147 molecules have multiple cellular functions, such as migration, adhesion, invasion, energy metabolism and T cell activation. In particular, recent studies have demonstrated the potential application of CD147 as an effective therapeutic target for cancer, as well as autoimmune and inflammatory diseases. In this study, we elucidated the functional epitopes on CD147 extracellular domains in T cell regulation using specific monoclonal antibodies (mAbs). Upon T cell activation, the anti-CD147 domain 1 mAbs M6-1E9 and M6-1D4 and the anti-CD147 domain 2 mAb MEM-M6/6 significantly reduced surface expression of CD69 and CD25 and T cell proliferation. To investigate whether functional epitopes of CD147 are differentially expressed on distinct leukocyte subsets, PBMCs, monocyte-depleted PBMCs and purified T cells were activated in the presence of anti-CD147 mAbs. The mAb M6-1E9 inhibited T cell functions via activation of CD147 on monocytes with obligatory cell-cell contact. Engagement of the CD147 epitope by the M6-1E9 mAb downregulated CD80 and CD86 expression on monocytes and IL-2, TNF-α, IFN-γ and IL-17 production in T cells. In contrast, the mAb M6-1D4 inhibited T cell function via activation of CD147 on T cells by downregulating IL-2, TNF-α and IFN-γ. Herein, we demonstrated that certain epitopes of CD147, expressed on both monocytes and T cells, are involved in the regulation of T cell activation.

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Section: Discussionmentioning

confidence: 76%

Section: Resultsmentioning

confidence: 99%

Differential CD147 Functional Epitopes on Distinct Leukocyte Subsets

et al. 2021

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Sandwich antibody-based biosensor system for identification of Mycobacterium tuberculosis complex and nontuberculous mycobacteria

Chuensirikulchai

Laopajon

Phunpae

et al. 2019

Journal of Immunoassay and Immunochemistry

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Role of MMP-2 and CD147 in kidney fibrosis

Cheng

Zhang

et al. 2022

Open Life Sciences

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Matrix metalloproteinase-2 (MMP-2) and cluster of differentiation 147 (CD147) both play important roles in the development of kidney fibrosis, and CD147 can induce the production and activation of MMP-2. In the early stage of kidney fibrosis, MMP-2 promotes extracellular matrix (ECM) production and accelerates the development of kidney fibrosis, while in the advanced stage, MMP-2 activity decreases, leading to reduced ECM degradation and making it difficult to alleviate kidney fibrosis. The reason for the decrease in MMP-2 activity in the advanced stage is still unclear. On the one hand, it may be related to hypoxia and endocytosis, which lead to changes in the expression of MMP-2-related active regulatory molecules; on the other hand, it may be related to insufficient CD147 function. At present, the specific process by which CD147 is involved in the regulation of MMP-2 activity is not completely clear, and further in-depth studies are needed to clarify the roles of both factors in the pathophysiology of kidney fibrosis.

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Antibody biosensors for the measurement and characterization of soluble CD147 molecules

Abstract: This method may be a beneficial tool for the determination of sCD147 isoforms for disease diagnosis and prognosis as well as for the definition of the cellular mechanisms of the immune system.

Cited by 3 publications

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Differential CD147 Functional Epitopes on Distinct Leukocyte Subsets

Differential CD147 Functional Epitopes on Distinct Leukocyte Subsets

Sandwich antibody-based biosensor system for identification of Mycobacterium tuberculosis complex and nontuberculous mycobacteria

Role of MMP-2 and CD147 in kidney fibrosis

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