Antibody-conjugated and streptomycin-chitosan oligosaccharide-modified gold nanoshells for synergistic chemo-photothermal therapy of drug-resistant bacterial infection

Manivasagan, Panchanathan; Khan, Fazlurrahman; Dhatchayeny, Durai Rajan; Park, Sumin; Joe, Ara; Han, Hyowon; Seo, Sun-Hwa; Thambi, Thavasyappan; Phan, Vu To-Anh; Kim, Young‐Mog; Kim, Chang-Seok; Oh, Junghwan; Jang, Eue‐Soon

doi:10.1016/j.jare.2022.08.009

Cited by 16 publications

(2 citation statements)

References 84 publications

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“… , Compared with the common photothermal reagents, the photothermal conversion efficiency of PSB was higher than that of most PT reagents, such as gold-based particles and indocyanine green (Table S1). − We also investigated the photothermal stability and viability of PSB, as these factors are crucial for maintaining the antigen transport ability of PSB following NIR light irradiation. As we expected, the UV–vis-NIR spectrum of PSB was not affected after NIR light irradiation (Figure S6).…”

Section: Resultsmentioning

confidence: 99%

Redirecting Antigens by Engineered Photosynthetic Bacteria and Derived Outer Membrane Vesicles for Enhanced Cancer Immunotherapy

Han,

Wang,

et al. 2023

ACS Nano

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Significant strides have been made in the development of cancer vaccines to combat malignant tumors. However, the natural immunosuppressive environment within tumors, known as the tumor microenvironment (TME), hampers the uptake and presentation of antigens by antigenpresenting cells (APCs) within the tumor itself. This limitation results in inadequate activation of immune responses against cancer. In contrast, immune cells in peritumoral tissue maintain their normal functions. In this context, we present an interesting approach to enhance cancer immunotherapy by utilizing engineered photosynthetic bacteria (PSB) and their outer membrane vesicles (OMV PSB ) to capture and transport antigens to the outer regions of the tumor. We modified PSB with maleimide (PSB-MAL), which, when exposed to near-infrared (NIR) laser-mediated photothermal therapy (PTT), induced extensive cancer cell death and the release of tumor antigens. Subsequently, the NIR-phototactic PSB-MAL transported these tumor antigens to the peripheral regions of the tumor under NIR laser exposure. Even more intriguingly, PSB-MAL-derived OMV PSB -MAL effectively captured and delivered antigens to tumor-draining lymph nodes (TDLNs). This facilitated enhanced antigen presentation by mature and fully functional APCs in the TDLNs. This intricate communication network between PSB-MAL, the OMV PSB -MAL, and APCs promoted the efficient presentation of tumor antigens in the tumor periphery and TDLNs. Consequently, there was a notable increase in the infiltration of cytotoxic T lymphocytes (CTLs) into the tumor, triggering potent antitumor immune responses in both melanoma and breast cancer models. This cascade of events resulted in enhanced suppression of tumor metastasis and recurrence, underscoring the robust efficacy of our approach. Our interesting study, harnessing the potential of bacteria and OMVs to redirect tumor antigens for enhanced cancer immunotherapy, provides a promising path toward the development of personalized cancer vaccination strategies.

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Section: Resultsmentioning

confidence: 99%

Redirecting Antigens by Engineered Photosynthetic Bacteria and Derived Outer Membrane Vesicles for Enhanced Cancer Immunotherapy

Han,

Wang,

et al. 2023

ACS Nano

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“…The aldehyde group of STR was combined with the free amino group of chitooligosaccharides (CO) to couple STR and CO. Then, the TGA was coupled to gold nanoshells via free sulfhydryl groups. Finally, the anti-Listeria monocytogenes (LM) antibody was loaded for precise localization to make the multi-functional antibacterial nanoplatforms anti-STR-CO-GNSs (Manivasagan et al, 2023). In the acidic infection environment, the targeted slow release of STR is achieved with the help of NIR irradiation, and the disruption of the cell membrane accelerates the penetration of STR into bacteria.…”

Section: Drug-pttmentioning

confidence: 99%

Nanotechnology‐driven strategies to enhance the treatment of drug‐resistant bacterial infections

Zhang,

Liu,

Guo

et al. 2024

WIREs Nanomed Nanobiotechnol

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The misuse of antibiotics has led to increased bacterial resistance, posing a global public health crisis and seriously endangering lives. Currently, antibiotic therapy remains the most common approach for treating bacterial infections, but its effectiveness against multidrug‐resistant bacteria is diminishing due to the slow development of new antibiotics and the increase of bacterial drug resistance. Consequently, developing new a\ntimicrobial strategies and improving antibiotic efficacy to combat bacterial infection has become an urgent priority. The emergence of nanotechnology has revolutionized the traditional antibiotic treatment, presenting new opportunities for refractory bacterial infection. Here we comprehensively review the research progress in nanotechnology‐based antimicrobial drug delivery and highlight diverse platforms designed to target different bacterial resistance mechanisms. We also outline the use of nanotechnology in combining antibiotic therapy with other therapeutic modalities to enhance the therapeutic effectiveness of drug‐resistant bacterial infections. These innovative therapeutic strategies have the potential to enhance bacterial susceptibility and overcome bacterial resistance. Finally, the challenges and prospects for the application of nanomaterial‐based antimicrobial strategies in combating bacterial resistance are discussed.This article is categorized under: Biology‐Inspired Nanomaterials > Nucleic Acid‐Based Structures Therapeutic Approaches and Drug Discovery > Nanomedicine for Infectious Disease

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Copper‐Cysteine Nanostructures for Synergetic Photothermal Therapy and Chemodynamic Therapy of Bacterial Skin Abscesses

Bagheri,

Bochani,

Seyedhamzeh

et al. 2024

Advanced Therapeutics

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Skin lesions, including skin bacterial abscesses, have become one of the most important health challenges and usually need systemic high‐dose antibiotics. Therefore, it is of particular importance to develop novel approaches for treating this ever‐growing challenge to human health. To address this challenge, herein a copper nanostructure is developed giving combined photothermal and chemodynamic therapies for focal infection treatment. The Cu‐based nanostructures with intrinsic catalytic properties are prepared by D‐L or L cysteine (Cys) as ligand and copper ions. It is shown that the multifunctional copper‐Cys (Cu‐Cys) nanostructures can produce reactive oxygen species (ROS) and they exhibit near infrared (NIR)‐enhanced catalytic activities to improve ROS production for highly efficient eradication of bacteria. Moreover, the results proved O2 evolution property of the Cu‐Cys nanoparticles (NPs). The nanostructures show shape‐dependent antibacterial activity where DL‐Cu‐Cys NPs show higher bactericidal performance than L‐Cu‐Cys NPs. In vitro results demonstrate that 2.5 and 1.25 µg mL−1 of DL‐Cu‐Cys NPs is enough to achieve rapid killing of Escherichia coli (E. coli) or Staphylococcus aureus (S. aureus) respectively under 808 nm light irradiation in 10 min. This work introduces a unique photoactive nanoagent to efficiently treat subcutaneous abscess by combining NIR light‐triggered photothermal effect and catalytic generation of ROS without using any antibiotic.

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Antibody-conjugated and streptomycin-chitosan oligosaccharide-modified gold nanoshells for synergistic chemo-photothermal therapy of drug-resistant bacterial infection

Cited by 16 publications

References 84 publications

Redirecting Antigens by Engineered Photosynthetic Bacteria and Derived Outer Membrane Vesicles for Enhanced Cancer Immunotherapy

Redirecting Antigens by Engineered Photosynthetic Bacteria and Derived Outer Membrane Vesicles for Enhanced Cancer Immunotherapy

Nanotechnology‐driven strategies to enhance the treatment of drug‐resistant bacterial infections

Copper‐Cysteine Nanostructures for Synergetic Photothermal Therapy and Chemodynamic Therapy of Bacterial Skin Abscesses

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