Antibody conjugated metal nanoparticle decorated graphene sheets for a mycotoxin sensor

Srivastava, Saurabh; Kumar, Vinod; Arora, Kamal; Singh, Chandan; Ali, Md. Azahar; Puri, Nitin K.; Malhotra, Bansi D.

doi:10.1039/c6ra04469g

Cited by 23 publications

(10 citation statements)

References 26 publications

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“…Modification of ITO electrodes by including metallic nanoparticles such as Au or Ni in the rGO coating leads to higher conductivity and increased active area. This was translated into larger heterogeneous electron transfer rate constants (i.e., faster kinetics of the electron transfer), enhanced sensitivity, and wider detection range compared to sensors lacking metallic nanoparticles [ 87 , 88 , 89 ]. More details on the integration of heterogeneous nanostructures with biomolecules are provided in Section 4 of this review.…”

Section: Role Of 2d Nanomaterials In Electrochemical Sensing Platformsmentioning

confidence: 99%

Two-Dimensional Nanostructures for Electrochemical Biosensor

Khan

Radoi

Rashid

et al. 2021

Sensors

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Current advancements in the development of functional nanomaterials and precisely designed nanostructures have created new opportunities for the fabrication of practical biosensors for field analysis. Two-dimensional (2D) and three-dimensional (3D) nanomaterials provide unique hierarchical structures, high surface area, and layered configurations with multiple length scales and porosity, and the possibility to create functionalities for targeted recognition at their surface. Such hierarchical structures offer prospects to tune the characteristics of materials—e.g., the electronic properties, performance, and mechanical flexibility—and they provide additional functions such as structural color, organized morphological features, and the ability to recognize and respond to external stimuli. Combining these unique features of the different types of nanostructures and using them as support for bimolecular assemblies can provide biosensing platforms with targeted recognition and transduction properties, and increased robustness, sensitivity, and selectivity for detection of a variety of analytes that can positively impact many fields. Herein, we first provide an overview of the recently developed 2D nanostructures focusing on the characteristics that are most relevant for the design of practical biosensors. Then, we discuss the integration of these materials with bio-elements such as bacteriophages, antibodies, nucleic acids, enzymes, and proteins, and we provide examples of applications in the environmental, food, and clinical fields. We conclude with a discussion of the manufacturing challenges of these devices and opportunities for the future development and exploration of these nanomaterials to design field-deployable biosensors.

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Section: Role Of 2d Nanomaterials In Electrochemical Sensing Platformsmentioning

confidence: 99%

Two-Dimensional Nanostructures for Electrochemical Biosensor

Khan

Radoi

Rashid

et al. 2021

Sensors

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“…The sensitivity of the method was 129.6 μA/[(ng·mL −1 )·cm 2 ] at 1–8 ng/mL. The high sensitivity was mainly attributed to the synergistic effect between rGO and Ni NPs [ 39 ]. Srivastava et al [ 44 ] used an electrophoretic deposition technique to deposit rGO on ITO conductive glass.…”

Section: Metal Oxides and Hydroxides Nanomaterials For Aflatoxins mentioning

confidence: 99%

Recent Advances in Aflatoxins Detection Based on Nanomaterials

et al. 2020

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Aflatoxins are the secondary metabolites of Aspergillus flavus and Aspergillus parasiticus and are highly toxic and carcinogenic, teratogenic and mutagenic. Ingestion of crops and food contaminated by aflatoxins causes extremely serious harm to human and animal health. Therefore, there is an urgent need for a selective, sensitive and simple method for the determination of aflatoxins. Due to their high performance and multipurpose characteristics, nanomaterials have been developed and applied to the monitoring of various targets, overcoming the limitations of traditional methods, which include process complexity, time-consuming and laborious methodologies and the need for expensive instruments. At the same time, nanomaterials provide general promise for the detection of aflatoxins with high sensitivity, selectivity and simplicity. This review provides an overview of recent developments in nanomaterials employed for the detection of aflatoxins. The basic aspects of aflatoxin toxicity and the significance of aflatoxin detection are also reviewed. In addition, the development of different biosensors and nanomaterials for aflatoxin detection is introduced. The current capabilities and limitations and future challenges in aflatoxin detection and analysis are also addressed.

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“…Various 2D nanomaterial-based electrochemical biosensors have been constructed for detecting AFB 1 in various matrixes [96][97][98][99][100][101][102][103][104][105][106][107][108][109][110][111][112][113]. Srivastava et al have developed a series of functionalized GO nanocomposite-based electrochemical biosensors for profiling AFB 1 in foods since they developed the first rGO-based AFB 1 immunosensor through the covalent conjugation of the monoclonal anti-AFB1 antibodies onto an rGO modified indium tin oxide (ITO) electrode in 2013 [96][97][98][99]. Among these electrochemical biosensors, the functionalized GO/rGO-based nanocomposites are employed in different roles, such as catalysts, electroactive probes and immobilization platforms for improving the biosensing performance.…”

Section: Aflatoxinsmentioning

confidence: 99%

“…Among these electrochemical biosensors, the functionalized GO/rGO-based nanocomposites are employed in different roles, such as catalysts, electroactive probes and immobilization platforms for improving the biosensing performance. For instance, benefiting from the highly crystalline properties of the rGO-Ni NPs sheets (Ni nanoparticle decorated rGO sheets) along with the excellent electro-catalytic properties, the rGO-Ni NPs-ITO-based AFB 1 immunosensor exhibits high sensitivity (129.6 mA ng −1 mL cm −2 ), long term stability (up to 6 weeks) and low LOD (0.16 ng mL −1 ) [99]. Photoelectrochemical (PEC) biosensors have attracted great attention in the biological analytical field as the PEC method can obtain high sensitivity without expensive equipment.…”

Section: Aflatoxinsmentioning

confidence: 99%

Two-Dimensional Layered Nanomaterial-Based Electrochemical Biosensors for Detecting Microbial Toxins

Jian

et al. 2019

Toxins

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Toxin detection is an important issue in numerous fields, such as agriculture/food safety, environmental monitoring, and homeland security. During the past two decades, nanotechnology has been extensively used to develop various biosensors for achieving fast, sensitive, selective and on-site analysis of toxins. In particular, the two dimensional layered (2D) nanomaterials (such as graphene and transition metal dichalcogenides (TMDs)) and their nanocomposites have been employed as label and/or biosensing transducers to construct electrochemical biosensors for cost-effective detection of toxins with high sensitivity and specificity. This is because the 2D nanomaterials have good electrical conductivity and a large surface area with plenty of active groups for conjugating 2D nanomaterials with the antibodies and/or aptamers of the targeted toxins. Herein, we summarize recent developments in the application of 2D nanomaterial-based electrochemical biosensors for detecting toxins with a particular focus on microbial toxins including bacterial toxins, fungal toxins and algal toxins. The integration of 2D nanomaterials with some existing antibody/aptamer technologies into electrochemical biosensors has led to an unprecedented impact on improving the assaying performance of microbial toxins, and has shown great promise in public health and environmental protection.

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Antibody conjugated metal nanoparticle decorated graphene sheets for a mycotoxin sensor

Abstract: The proposed rGO–Ni NPs based immunosensor utilized for aflatoxin B1 detection indicates high sensitivity.

Cited by 23 publications

References 26 publications

Two-Dimensional Nanostructures for Electrochemical Biosensor

Two-Dimensional Nanostructures for Electrochemical Biosensor

Recent Advances in Aflatoxins Detection Based on Nanomaterials

Two-Dimensional Layered Nanomaterial-Based Electrochemical Biosensors for Detecting Microbial Toxins

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