Antibody Dependent Cytotoxicity of Human Colostrum Phagocytes

Abstract

Summaryhave given contradicting results. Robinson et al. (30) found macrophages and neutrophils to phagocytose and kill Staph aureus MOnOc~tes and ~~~~~o~~~o~u c~~a~ neutro~hils of human ~010s-and E, co[i in vi[ro very efficiently after opsonization by the trum were identified using cytochemical staining procedures. The aqueous phase of milk. On the other hand Ho and Lawton (12) cO1Ostral phagocytes in antibody dependent found good phagocytic power of colostral cells but poor killing cellular cytotoxicity was found to be satisfactory at high effector/ activity of E, coli, target cells ratio (20:l) Colostral monocytes showed a diminishedThe aim of the present work was to further study the function cytotoxicity when 'Ompared mOnOcytes of Or of colostral phagocytes (monocytes and polymorphonuclear neuperipheral blood at low effector/target cells ratio (1:l). This could trophils). We tested their ability to elicit antibody-dependent be due to the extensive fat ingestion by these Or other cellular cytotoxicity i.e., their ability to kill anitbody-coated target inhibitory factors found in colostrum.cells in vitro.We also tested the ability of peripheral blood monocytes who Speculation had phagocytosed lipid to elicit antibody-dependent cytotoxicity.The demonstration of antibody dependent cellular cytotoxicity of colostral phagocytes gives further support to the biological MATERIALS AND METHODS importance of the cellular components of human milk.Collection of colostrum. Colostrum was obtained from 16 women within the first 5 days following delivery. Mothers manually There is now growing information documenting that human expressed their milk into sterile plastic containers. The samples breast milk is superior to cow's milk in minimizing both the were brought to the laboratory within one hour of collection. morbidity and mortality of infants (3,4,29,35). The immunologic Preparation of colostal cells. Because of the high fat content of factors in human milk that offer resistance against infection to the colostrum, the usual procedures for identification of the white neonates are summarized in several review articles (26,28,34). In cells in the peripheral blood could not be applied. Consequently contrast the functions of the cellular components of human milk alternate methods were devised. Samples were centrifused at 200 have received relatively little attention till recently. The white cells x g for 25 min. During centrifugation the milk separated into of human colostrum were first observed in 1839 by Alexander three layers: a cell pellet, middle layer and fat layer. The cell pellet Donne (6). These cells were rediscovered in 1966 by Smith work-was washed twice in Eagle's basal medium (BME) and resusing in Goldman's laboratory (31). They were found to be present pended in the same medium plus 10% foetal calf serum (FCS) at concentrations of 0.5-10 million cells/ml in colostrum and Subsequently the cells were counted in a haemocytometer. The comprise 30-85% macrophages, 1-80% polymorphonuclears and viability of cells...

The Effect of IFN-γ and TNF-α on the NADPH Oxidase System of Human Colostrum Macrophages, Blood Monocytes, and THP-1 Cells

“…(10) They represent 30%-80% of the human colostrum leukocyte population, which varies from 500 to 10,000 cells/ml. (3) CM cells express MHC class II molecules, (11) develop antibody-dependent cellular cytotoxicity, (12,13) ingest and kill bacteria, (14) and display the morphology and motility of activated cells. (3) Colostrum is an important and accessible source of human macrophages.…”

Colostro bovino: muito além das imunoglobulinas

“…Os leucócitos foram observados pela primeira vez no colostro humano por Alexander Donné (1844) apud Mandyla et al (1982).…”

Human milk cells

“…tokincs and to support antigenically mediated proliferative rc.\ponsc\ u hen cultured with autologous T cells (6). Both pol) morphonuclear neutrophils and macrophages in human milk ha\e been revealed to be able to phagocytose iuict kill bacteria and fungi ( 7 ) and elicit antibody-dependent c).totouicity (8).…”