Antibody Diversity: Something for Everyone

Capra, J D; Kindt, Thomas J.

doi:10.1111/j.1365-3083.1981.tb00126.x

Scand J Immunol

1981

DOI: 10.1111/j.1365-3083.1981.tb00126.x

|View full text |Cite

Antibody Diversity: Something for Everyone

J D Capra

Thomas J. Kindt

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1983

2002

Publication Types

Select...

Article3

Relationship

Self Cite0

Independent3

Authors

Journals

Cited by 3 publications

(2 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In somatic cells, an ever-increasing body of evidence supports an important role for homologous recombination in remodelling of the chromosomal Ig genes. Gene conversion is important in generating Ab diversity in birds and rabbits (10,11), and may also play a role in the diversification of some mammalian Ab responses (12)(13)(14)(15)(16)(17)(18)(19)(20). Both gene conversion and crossing over occur between Ig H chain constant segments within regions of shared homology (21)(22)(23)(24).…”

mentioning

confidence: 99%

Evidence for the Murine IgH μ Locus Acting as a Hot Spot for Intrachromosomal Homologous Recombination

Raynard

Read

Baker

2002

The Journal of Immunology

View full text Add to dashboard Cite

Homologous recombination accomplishes the exchange of genetic information between two similar or identical DNA duplexes. It can occur either by gene conversion, a process of unidirectional genetic exchange, or by reciprocal crossing over. Homologous recombination is well known for its role in generating genetic diversity in meiosis and, in mitosis, as a DNA repair mechanism. In the immune system, the evidence suggests a role for homologous recombination in Ig gene evolution and in the diversification of Ab function. Previously, we reported the occurrence of homologous recombination between repeated, donor and recipient alleles of the Ig H chain μ gene C (Cμ) region residing at the Ig μ locus in mouse hybridoma cells. In this study, we constructed mouse hybridoma cell lines bearing Cμ region heteroalleles to learn more about the intrachromosomal homologous recombination process. A high frequency of homologous recombination (gene conversion) was observed for markers spanning the entire recipient Cμ region, suggesting that recombination might initiate at random sites within the Cμ region. The Cμ region heteroalleles were equally proficient as either conversion donors or recipients. Remarkably, when the same Cμ heteroalleles were tested for recombination in ectopic genomic positions, the mean frequency of gene conversion was reduced by at least 65-fold. These results are consistent with the murine IgH μ locus behaving as a hot spot for intrachromosomal homologous recombination.

show abstract

mentioning

confidence: 99%

Evidence for the Murine IgH μ Locus Acting as a Hot Spot for Intrachromosomal Homologous Recombination

Raynard

Read

Baker

2002

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…An uneasy consensus of opinion is being reached concerning the genetic basis of antibody diversity (1). This consensus, which includes elements of both the original "germline" and "somatic diversification" models, is being built in large part through the characterization of antibodies elicited by simple haptens in mice.…”

mentioning

confidence: 99%

Amino acid sequence diversity within the family of antibodies bearing the major antiarsonate cross-reactive idiotype of the A strain mouse.

Slaughter¹,

Capra²

1983

The Journal of Experimental Medicine

View full text Add to dashboard Cite

VH region amino acid sequences are described for five A/J anti-p-azophenylarsonate (anti-Ars) hybridoma antibodies for which the VL region sequences have previously been determined, thus completing the V domain sequences of these molecules. These antibodies all belong to the family designated Ars-A which bears the major anti-arsonate cross-reactive idiotype (CRI) of the A strain mouse. However, they differ in the degree to which they express the CRI in standard competition radioimmunoassays. Although the sequences are closely related, all are different from each other. Replacements are distributed throughout the VH region and occur in positions of the chain encoded by all three gene segments, VH, DH, and JH. It is likely that somatic diversification processes play a dominant role in producing the sequence variability in each of these segments. The number of differences from the sequence encoded by the germline is smallest for antibodies that express the CRI most strongly, suggesting that somatic diversification is responsible for loss of the CRI in members of the Ars-A antibody family. There is an unusual degree of clustering of differences in both CDR2 and CDR3 and many of the substitutions are located in "hot spots" of variation. The large number of differences between the chains prohibits the unambiguous identification of positions at which alterations play a major role in reducing the expression of the CRI. However, the data suggest that the loss of the CRI is associated with a definable repertoire of somatic changes at a restricted number of highly variable sites.

show abstract

Rapid changes in specificity within single clones of cytolytic effector cells

Reimann

Miller

1985

Cell

View full text Add to dashboard Cite

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Antibody Diversity: Something for Everyone

Cited by 3 publications

References 19 publications

Evidence for the Murine IgH μ Locus Acting as a Hot Spot for Intrachromosomal Homologous Recombination

Evidence for the Murine IgH μ Locus Acting as a Hot Spot for Intrachromosomal Homologous Recombination

Amino acid sequence diversity within the family of antibodies bearing the major antiarsonate cross-reactive idiotype of the A strain mouse.

Rapid changes in specificity within single clones of cytolytic effector cells

Contact Info

Product

Resources

About