Antibody–drug gold nanoantennas with Raman spectroscopic fingerprints for in vivo tumour theranostics

Conde, João; Bao, Chunyan; Cui, Daxiang; Baptista, Pedro V.; Tian, Furong

doi:10.1016/j.jconrel.2014.03.045

Cited by 101 publications

(82 citation statements)

References 29 publications

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“…At a density of 1x10 4 per well, cells were seeded (in a 6-well Petridish). After exposure to GNPs at 0.0001 to 1 mg/mL for 10 days, cells (which were grown in colonies) were stained violet in colour with Carbol Fuchsin Solution (VWR international, Ltd. England).…”

Section: Biocompatibility Testsmentioning

confidence: 99%

“…Calu-3 cells were seeded at a density of 1x10 4 Subsequently image analysis was performed (i.e. quantification of fluorescent density) with ImageJ (a Java-based software freely available at NIH, version 1.45s), according to the procedure described in [26].…”

Section: Assessment Of Plasma Membranementioning

confidence: 99%

“…Due to their plasmonic properties, gold nanoparticles (GNPs) have been proposed as advantageous nano-sized materials for use in the various diagnostic and therapeutic applications, such as cell imaging, targeted drug delivery, thermal ablation, phototherapy [1][2][3][4][5][6][7].. To date spherical GNPs have been proven as one of the most biocompatible NP models for biological-based applications [8][9][10][11][12]. Recently, it has been reported that the specific shape of GNPs can be exploited to advantageously tune these nanomaterials for any biomedical application of choice [8,13].…”

Section: Introductionmentioning

confidence: 99%

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Investigating the Role of Shape on the Biological Impact of Gold Nanoparticles in Vitro

Tian

Clift

Casey³

et al. 2015

Nanomedicine (Lond.)

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Aim: To investigate the influence of gold nanoparticle (GNP) geometry on the biochemical response of Calu-3 epithelial cells. Materials and Methods:Spherical, triangular and hexagonal GNPs were used. The GNP-cell interaction was assessed via atomic absorption spectroscopy (AAS) and transmission electron microscopy (TEM). The biochemical impact of GNPs was determined over 72hrs at [0.0001-1mg/mL].Results: At 1mg/mL, hexagonal GNPs reduced Calu-3 viability below 60%, showed increased reactive oxygen species production and higher expression of pro-apoptotic markers. A cell mass burden of 1:2:12 as well as number of GNPs per cell (2:1:3) was observed for spherical:triangular:hexagonal GNPs. Conclusion:These findings do not suggest a direct shape-toxicity effect. However, do highlight the contribution of shape towards the GNP-cell interaction which impacts upon their intracellular number, mass and volume dose.

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Section: Biocompatibility Testsmentioning

confidence: 99%

Section: Assessment Of Plasma Membranementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Investigating the Role of Shape on the Biological Impact of Gold Nanoparticles in Vitro

Tian

Clift

Casey³

et al. 2015

Nanomedicine (Lond.)

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“…Bimetallic Au and Ag nanomaterials offer excellent features in terms of chemical and plasmonic properties. Previous studies showed that Au/Ag bimetallic nanoparticles exhibit higher SERS activity than the pure Au or Ag nanoparticles, and can be used for ultrasensitive detection of biomarkers [37][38][39]. However, up to date, few report is associated with the use of bimetallic Ag@Au nanorods as theranostic agent for in vivo targeted SERS imaging for tumor boundary identification, photoacoustic imaging and simultaneous photothermal therapy.…”

Section: Introductionmentioning

confidence: 99%

EGFR Antibody Conjugated Bimetallic Au@Ag Nanorods for Enhanced SERS-Based Tumor Boundary Identification, Targeted Photoacoustic Imaging and Photothermal Therapy

Chen¹,

Zhang²,

Yang³

et al. 2016

Nano BioMed ENG

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The development of multifunctional nanoprobes for simultaneous targeted imaging, tumor boundary identification and photothermal therapy of gastric cancer has become a great challenge. Herein, EGFR monoclonal antibody-conjugated Au@Ag nanorods decorated with DTNB nanoprobes (5,5'-Dithiobis-(2-nitrobenzoic acid)) (EGFR-Au@Ag NR nanotags) were prepared and characterized. Their biocompatibility was analyzed by MTT assay. In vitro studies show that EGFR-Au@Ag NR nanotags own good biocompatibility and capability of targeting and entering into gastric cancer MGC803 cells, silver nano-film layer on the surface of gold nanorods remarkably enhance surfaceenhanced rama spectra (SERS) signal, enhanced photoacoustic imaging efficacy and photothermal conversion efficiency of gold nanorods. In vivo studies show that prepared nanotags could target actively gastric cancer cells at 2 h post-injection, and distributed in the tumor site, exhibited enhanced SERS signals to display clearly tumor boundary, enhanced photoacoustic imaging to display clearly tumor boundary. Under 1 w/cm 2 laser irradiation, tumor growth was remarkably inhibited by local photothermal therapy. In conclusion, high performance EGFR-antibody conjugated Au@Ag nanorod-DTNB nanoprobes exhibit great potential in applications such as targeted photoacoustic imaging, simultaneous tumor boundary identification and selective photothermal therapy of gastric cancer in the near future.

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“…10,11 Gold nanoparticles have been extensively studied as potential SERS contrast agents for cancer diagnosis. 7,[10][11][12][13][14][15][16] The SERS imaging technique depends on the enhancement factor of the nanoparticle's Raman signal by creating local high electric fields.…”

Section: Introductionmentioning

confidence: 99%

Development of nanostars as a biocompatible tumor contrast agent: toward in vivo SERS imaging

D’Hollander¹,

Mathieu²,

Jans³

et al. 2016

IJN

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The need for sensitive imaging techniques to detect tumor cells is an important issue in cancer diagnosis and therapy. Surface-enhanced Raman scattering (SERS), realized by chemisorption of compounds suitable for Raman spectroscopy onto gold nanoparticles, is a new method for detecting a tumor. As a proof of concept, we studied the use of biocompatible gold nanostars as sensitive SERS contrast agents targeting an ovarian cancer cell line (SKOV3). Due to a high intracellular uptake of gold nanostars after 6 hours of exposure, they could be detected and located with SERS. Using these nanostars for passive targeting after systemic injection in a xenograft mouse model, a detectable signal was measured in the tumor and liver in vivo. These signals were confirmed by ex vivo SERS measurements and darkfield microscopy. In this study, we established SERS nanostars as a highly sensitive contrast agent for tumor detection, which opens the potential for their use as a theranostic agent against cancer.

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Antibody–drug gold nanoantennas with Raman spectroscopic fingerprints for in vivo tumour theranostics

Cited by 101 publications

References 29 publications

Investigating the Role of Shape on the Biological Impact of Gold Nanoparticles in Vitro

Investigating the Role of Shape on the Biological Impact of Gold Nanoparticles in Vitro

EGFR Antibody Conjugated Bimetallic Au@Ag Nanorods for Enhanced SERS-Based Tumor Boundary Identification, Targeted Photoacoustic Imaging and Photothermal Therapy

Development of nanostars as a biocompatible tumor contrast agent: toward in vivo SERS imaging

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