Antibody engineering

Hayden, Martha S.; Gilliland, Lisa K.; Ledbetter, Jeffrey A.

doi:10.1016/s0952-7915(97)80136-7

Cited by 83 publications

(44 citation statements)

References 104 publications

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“…irus infection can initiate various antiviral signaling pathways, including the nuclear factor kappa B (NF-B) pathway (1)(2)(3)(4). The NF-B family plays an important role in inflammation and the innate immune response and is composed of five transcription factors, including RelA/p65, RelB, c-Rel, p50/NF-B1, and p52/ NF-B2, that homo-and heterodimerize (3).…”

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confidence: 99%

Ectromelia Virus Encodes a BTB/kelch Protein, EVM150, That Inhibits NF-κB Signaling

Wang

Burles

Couturier

et al. 2014

J Virol

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The NF-B signaling pathway plays a critical role in inflammation and innate immunity. Consequently, many viruses have evolved strategies to inhibit NF-B in order to facilitate replication and evasion of the host immune response. Recently, we determined that ectromelia virus, the causative agent of mousepox, contains a family of four BTB/kelch proteins that interact with cullin-3-based ubiquitin ligases. We demonstrate here that expression of EVM150, one of the four BTB/kelch proteins, inhibited NF-B activation induced by tumor necrosis factor alpha (TNF-␣) and interleukin-1␤ (

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confidence: 99%

Ectromelia Virus Encodes a BTB/kelch Protein, EVM150, That Inhibits NF-κB Signaling

Wang

Burles

Couturier

et al. 2014

J Virol

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“…항체 공학을 이용해 제조되는 재조합항체는 특이적 항원 결합력을 갖는 Fv 항체와 Single-chain Fv(ScFv) 항체 (Hayden et al, 1997;Verma et al, 1998) (Bird et al, 1988;Huston et al, 1991 (Nishinaka, et al, 1996) (Fig. 1 and Fig.…”

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The Development of Chicken Recombinant Single-chain Fv (ScFv) Antibody Reactive with Sporozoite Antigen of Eimeria spp. which Causes Avian Coccidiosis

Park¹,

Kim

Kim³

et al. 2011

Korean Journal of Poultry Science

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The chicken monoclonal antibody (mAb), 13C8, reacts with sporozoite antigens of Eimeria spp. which causes avian coccidiosis. Since this mAb was produced at low amount due to genetic instability of chicken hybridoma, a recombinant 13C8 single-chain Fv (ScFv) antibody was constructed by amplification of the variable domain of heavy (VH) and light chain (VL) genes of antibody derived from chicken hybridoma. The constructed 13C8 ScFv was successfully expressed in E. coli and purified as a soluble form. In ELISA analysis, this recombinant 13C8 ScFv antibody showed antigen binding activity as the original mAb. In addition, nucleotide sequence comparison of 13C8 gene to the germline chicken VL and VH genes suggested that the gene conversion with Vλ and VH pseudogenes might contribute to the diversification of VL and VH genes in chickens.(

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“…For example, modifications may be made to modulate target binding and pharmacokinetic properties, increase the degree of humanness, or address developability issues that might affect drug safety and shelf life. [8][9][10][11] Many of those tasks are enabled, or at least greatly facilitated, by structure-based considerations on the atomic level. 12 The large number of publicly available antibody crystal structures that have been solved during the last 25 y is an indicator for the relevance of this class of proteins.…”

Section: Introductionmentioning

confidence: 99%

MoFvAb: Modeling the Fv region of antibodies

Bujotzek

Fuchs

et al. 2015

mAbs

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Knowledge of the 3-dimensional structure of the antigen-binding region of antibodies enables numerous useful applications regarding the design and development of antibody-based drugs. We present a knowledge-based antibody structure prediction methodology that incorporates concepts that have arisen from an applied antibody engineering environment. The protocol exploits the rich and continuously growing supply of experimentally derived antibody structures available to predict CDR loop conformations and the packing of heavy and light chain quickly and without user intervention. The homology models are refined by a novel antibody-specific approach to adapt and rearrange sidechains based on their chemical environment. The method achieves very competitive all-atom root mean square deviation values in the order of 1.5 A on different evaluation datasets consisting of both known and previously unpublished antibody crystal structures.

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Antibody engineering

Cited by 83 publications

References 104 publications

Ectromelia Virus Encodes a BTB/kelch Protein, EVM150, That Inhibits NF-κB Signaling

Ectromelia Virus Encodes a BTB/kelch Protein, EVM150, That Inhibits NF-κB Signaling

The Development of Chicken Recombinant Single-chain Fv (ScFv) Antibody Reactive with Sporozoite Antigen of Eimeria spp. which Causes Avian Coccidiosis

MoFvAb: Modeling the Fv region of antibodies

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