Antibody immobilization on gold nanoparticles coated layer-by-layer with polyelectrolytes

Masereel, Bernard; Dinguizli, Mustapha; Bouzin, Caroline; Moniotte, Nicolas; Feron, Olivier; Gallez, Bernard; Borght, Thierry Vander; Michiels, Carine; Lucas, Stéphane

doi:10.1007/s11051-010-9908-3

Cited by 45 publications

(31 citation statements)

References 29 publications

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“…An anionic polyelectrolyte can then be deposited as a second layer, again inverting the overall surface charge. This process is repeated for any number of layers whereby the final surface characteristics of the NP-polyelectrolyte hybrid are determined by the final layer (Decher 1997;Masereel et al 2011). Appropriate polyelectrolytes must be chosen when working with biological samples.…”

Section: Methodsmentioning

confidence: 99%

Improving biohydrogen production using Clostridium beijerinckii immobilized with magnetite nanoparticles

Seelert

Ghosh

Yargeau

2015

Appl Microbiol Biotechnol

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In order to supplement the need for alternative energy resources within the near future, enhancing the production of biohydrogen with immobilized Clostridium beijerinckii NCIMB8052 was investigated. Magnetite nanoparticles were functionalized, with chitosan and alginic acid polyelectrolytes using a layer-by-layer method, to promote bacterial attachment. Cultivating C. beijerinckii with these nanoparticles resulted in a shorter lag growth phase and increased total biohydrogen production within 100-ml, 250-ml and 3.6-L reactors compared with freely suspended organisms. The greatest hydrogen yield was obtained in the 250-ml reactor with a value of 2.1±0.7 mol H 2 /mol glucose, corresponding to substrate conversion and energy conversion efficiencies of 52±18 and 10±3 %, respectively. The hydrogen yields obtained using the immobilized bacteria are comparable to values found in literature. However, to make this process viable, further improvements are required to increase the substrate and energy conversion efficiencies.

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Section: Methodsmentioning

confidence: 99%

Improving biohydrogen production using Clostridium beijerinckii immobilized with magnetite nanoparticles

Seelert

Ghosh

Yargeau

2015

Appl Microbiol Biotechnol

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“…Carboxylmethylated pullulan (CMP) is an anionic derivative of pullulan, which has been widely explored for biomedical applications (Dulong et al 2012;Pereiraa, et al 2014;Mocanu et al 2014). Polyallylamine (PAA) was selected as a model cationic polymer which contains high density primary amino groups (Masereel et al 2011) that give positive charge to form NCs with anionic pullulan and bind with negatively charge DNA.…”

Section: Introductionmentioning

confidence: 99%

Self-assembled nanocomplexes of anionic pullulan and polyallylamine for DNA and pH-sensitive intracellular drug delivery

et al. 2014

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The amalgamation of chemotherapy and gene therapy is promising treatment option for cancer. In this study, novel biocompatible self-assembled nanocomplexes (NCs) between carboxylmethylated pullulan t335 (CMP) with polyallylamine (CMP-PAA NCs) were developed for plasmid DNA (pDNA) and pH-sensitive doxorubicin (DOX) delivery. DOX was conjugated to CMP (DOX-CMP) via hydrazone and confirmed by FTIR and 1 H-NMR. In vitro release studies of pH-sensitive DOX-CMP conjugate showed 23 and 85 % release after 48 h at pH 7.4 (physiological pH) and pH 5 (intracellular/tumoral pH), respectively. The CMP-PAA NCs or DOX-CMP-PAA NCs self-assembled into a nanosized (\250 nm) spherical shape as confirmed by DLS and TEM. The hemolysis and cytotoxicity study indicated that the CMP-PAA NCs did not show cytotoxicity in comparison with plain polyallylamine. Gel retardation assay showed complete binding of pDNA with CMP-PAA NCs at 1:2 weight ratio. CMP-PAA NCs/pDNA showed significantly higher transfection in HEK293 cells compared to PAA/pDNA complexes. Confocal imaging demonstrated successful cellular uptake of DOX-CMP-PAA NCs in HEK293 cells. Thus, NCs hold great potential for targeted pDNA and pH-sensitive intratumoral drug delivery.

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“…These results strongly suggest that anti-diphtheria IgG is covalently bonded to GNPs without any negative alteration in the efficiency of the immunological interaction [18]. According to the ELISA results, using the ELISA method is not appropriate in order for the low concentration detection of toxoid.…”

Section: Elisa Measurementsmentioning

confidence: 94%

“…No shift in the λmax of fluorescence indicates that the polarity of the tryptophan environment and hence the overall protein structure did not change upon conjugation. It may suggest that the fluorescence intensity of the antibody has been quenched by GNPs [18]. Therefore, the CD and fluorescence results revealed that upon the covalent interaction between the antibody and GNPs, the structure of the antibodies has been somewhat changed.…”

Section: Structure Of Antibody Tracing After Bioconjugationmentioning

confidence: 99%

“…According to the Masereel et al method [18], antibody was bioconjugated using chemical linkers (e.g., EDC/NHS) which cause covalent conjugation. This controllable reaction is more specific so the older absorption method and the number of covalently conjugated ligands can be optimized to the particular application.…”

Section: Functionalization Of Gnps With Monoclonal Diphtheria Antibodmentioning

confidence: 99%

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Localized Surface Plasmon Resonance (LSPR) Detection of Diphtheria Toxoid Using Gold Nanoparticle-Monoclonal Antibody Conjugates

et al. 2017

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Detection of diphtheria toxin (DT) which is produced by Corynebacterium diphtheria, a zoonotic pathogen and a leading cause of diphtheria, is the critical step in the clinical laboratory. Traditional methods for DT detection are time consuming with low sensitivity. Herein, a localized surface plasmon resonance (LSPR) nanobioprobe has been developed based on specific immunological interactions between gold nanoparticles (GNPs) conjugated with monoclonal antibody and diphtheria toxoid in order for the rapid detection of DT. For this, plasmonic GNPs were conjugated to monoclonal antibodies covalently. The covalent conjugation has been confirmed by dynamic light scattering (DLS) and electrochemical techniques. Then, structural alterations of the conjugated antibody were monitored by circular dichroism (CD) and fluorescence spectroscopy methods. After that, the sensitivity of the nanobioprobe has been investigated via measuring the LSPR band λmax shifts of GNPs and LSPR sensitivity of nanobioprobe was compared with the ELISA method. Results suggested that this assay is highly selective and sensitive with a lower detection limit of about 10 ng/mL. The LSPR biosensor reduced the DT detection time from 2 or 3 days to less than 1 h compared with traditional methods. In conclusion, the investigation presents a rapid, sensitive, and selective method for the diagnosis of DT in clinical specimens.

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Antibody immobilization on gold nanoparticles coated layer-by-layer with polyelectrolytes

Cited by 45 publications

References 29 publications

Improving biohydrogen production using Clostridium beijerinckii immobilized with magnetite nanoparticles

Improving biohydrogen production using Clostridium beijerinckii immobilized with magnetite nanoparticles

Self-assembled nanocomplexes of anionic pullulan and polyallylamine for DNA and pH-sensitive intracellular drug delivery

Localized Surface Plasmon Resonance (LSPR) Detection of Diphtheria Toxoid Using Gold Nanoparticle-Monoclonal Antibody Conjugates

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