Antibody isotype responses to experimental infection with bovine herpesvirus 1 in calves with colostrally derived antibody

Bradshaw, Bernard; Edwards, S.

doi:10.1016/s0378-1135(96)01242-4

Cited by 28 publications

(23 citation statements)

References 18 publications

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“…The presence of maternal antibodies did not prevent the shedding of virus and the establishment of a latent state after BHV-1 infection, as has already been demonstrated by Lemaire and others (1995) and Bradshaw and Edwards (1996). After infection with a moderate dose of the virulent field strain Ciney of BHV-1, gE-negative passively immunised calves excreted large amounts of virus (from 103-3 to 106-5 PFU/100 mg of nasal secretions) for between seven and eight days, a similar period to that observed after the inoculation of passively immunised calves with higher titres of BHV-1 (Lemaire andothers 1995, Bradshaw andEdwards 1996).…”

Section: Discussionsupporting

confidence: 69%

“…After infection with a moderate dose of the virulent field strain Ciney of BHV-1, gE-negative passively immunised calves excreted large amounts of virus (from 103-3 to 106-5 PFU/100 mg of nasal secretions) for between seven and eight days, a similar period to that observed after the inoculation of passively immunised calves with higher titres of BHV-1 (Lemaire andothers 1995, Bradshaw andEdwards 1996). However, in this study, a prolonged virus shedding at low titres was observed (up to 19 days after inoculation).…”

Section: Discussionmentioning

confidence: 61%

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Antibody response to glycoprotein E after bovine herpesvirus type 1 infection in passively immunised, glycoprotein E‐negative calves

et al. 1999

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This study was conducted to determine whether young calves with maternal antibodies against bovine herpesvirus type 1 (BHV-1) but without antibodies against glycoprotein E (gE) can produce an active antibody response to gE after a BHV-1 infection. Five calves received at birth colostrum from gE-seronegative cows which had been vaccinated two or three times with an inactivated BHV-1, gE-deleted marker vaccine. After inoculation with a wild-type virulent strain of BHV-1, all the passively immunised gE-negative calves shed virus in large amounts in their nasal secretions. All the calves seroconverted to gE within two to four weeks after inoculation and then had high levels of gE antibodies for at least four months. The development of an active cell-mediated immune response was also detected by in vitro BHV-1-specific interferon-gamma assays. All the calves were latently infected, because one of them re-excreted the virus spontaneously and the other four did so after being treated with dexamethasone. The results showed that under the conditions of this work the gE-negative marker could also distinguish between passively immunised and latently infected calves.

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Section: Discussionsupporting

confidence: 69%

Section: Discussionmentioning

confidence: 61%

Antibody response to glycoprotein E after bovine herpesvirus type 1 infection in passively immunised, glycoprotein E‐negative calves

et al. 1999

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“…First, the IgM response to BHV-1 is less susceptible to suppression in the presence of maternally derived antibody than is the specific IgG response. 2 Second, if the acute serum sample is collected too late in the course of infection, the subsequent increase in BHV-1 IgG levels may be insufficient to demonstrate a 4-fold rise in titer. Third, individual animals may have detectable BHV-1 IgM in their convalescent serum samples without seroconversion because infection actually occurred in the intersample interval.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of an Immunofluorescent Antibody Test to Detect Bovine Herpesvirus 1-Specific IgM

Graham¹,

Foster²,

German³

et al. 1999

J VET Diagn Invest

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Abstract. An indirect immunofluorescent antibody test (IIFAT) was developed to detect bovine herpesvirus 1 (BHV-1)-specific IgM. All sera were treated with protein-G agarose prior to testing to eliminate the possibility of false-positive results due to IgM-isotype rheumatoid factor (IgM-RF). Specific IgM was first detected 8 days after experimental infection of 3 calves free of maternally derived antibody, with peak responses occurring 2-7 days later. Seroconversion was detected in all 3 calves using a single-dilution enzyme-linked immunosorbent assay. Following reinfection at 30 days postinfection, a low-level IgM response was detected in only 1 calf. Seroconversion was detected in 2 calves. There was no evidence of activation of IgM-RF by infection or reinfection with BHV-1. When 87 acute and convalescent serum pairs collected from 21 outbreaks of respiratory disease were tested, specific IgM was detected in 58 animals (66.6%) from 19 (90.5%) outbreaks. Seroconversion was detected in 44 of these animals (50.6%) from 17 outbreaks (81.0%). The correlations between these 2 assays on a calf and outbreak basis were 79.3% and 90.5%, respectively. Specific IgM was detected in 17/ 20 sera (85.0%) collected from an additional outbreak. No virus was detected by virus isolation or immunofluorescent staining in nasal mucus samples collected at the same time. Detection of specific IgM by IIFAT is a useful technique for the serodiagnosis of BHV-1 infection.

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“…Distinct subtypes have also been related to somewhat different clinical syndromes. Thus, typical or classical BoHV1 strains, presently classified as subtype 1 (BoHV1.1), have been associated to respiratory and genital disease and abortions (1, 9, 11), subtype 1.2a has been associated to respiratory disease and abortions (1, 4, 13), whereas BoHV1.2b has been associated to genital disease but to date never linked to abortions (2, 10, 11). …”

mentioning

confidence: 99%

“…After a primary experimental infection -or vaccination - calves develop an immune response revealed by a transient rise in specific IgM and IgA antibodies, followed by IgG1 and IgG2 responses (3, 5, 7, 8). BoHV1.2b induces seems to induce a lower degree of stimulation of humoral immune response, as estimated by the comparative amount of specific antibody production when compared to BoHV1.1 (1). BoHV1.2a immune responses have not been studied in detail.…”

mentioning

confidence: 99%

Analysis of isotype-specific antibody responses to bovine herpesviruses 1.1 and 1.2a allows to estimate the stage of infection

Spilki

Franco

Roehe

2012

Braz. J. Microbiol.

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Specific IgM, IgA, IgG1, IgG2, as well as neutralizing antibody responses were evaluated in sera of calves experimentally infected with two isolates of bovine herpesvirus type 1 (BoHV1) of distinct subtypes (subtype 1, BoHV1.1; subtype 2a, BoHV-1.2a). No significant differences were observed in the antibody responses induced by each BoHV-1 subtype. The antibody responses following primary acute infection were characterized by an increase in specific IgM and IgA levels between days 2 and 14 post inoculation (pi). IgG1 was detected from days 11 to 30 pi. IgG2 was detected on the sample taken on day 30 pi. Reactivation of infection following dexamethasone administration induced a significant rise in IgA levels, whereas IgG1 and IgG2 levels, which were at high levels from the beginning of the reactivation process, showed a slight alteration after corticosteroid treatment. These results suggest that it is possible to estimate the dynamics of BoHV-1 infections with basis on the analysis of class- and subclass-specific antibody responses. Such information may be particularly useful for the study of the kinetics of the infection in a herd and to aid in the adoption of appropriate control measures..

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Antibody isotype responses to experimental infection with bovine herpesvirus 1 in calves with colostrally derived antibody

Cited by 28 publications

References 18 publications

Antibody response to glycoprotein E after bovine herpesvirus type 1 infection in passively immunised, glycoprotein E‐negative calves

Antibody response to glycoprotein E after bovine herpesvirus type 1 infection in passively immunised, glycoprotein E‐negative calves

Evaluation of an Immunofluorescent Antibody Test to Detect Bovine Herpesvirus 1-Specific IgM

Analysis of isotype-specific antibody responses to bovine herpesviruses 1.1 and 1.2a allows to estimate the stage of infection

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