Antibody-modified reduced graphene oxide film for circulating tumor cell detection in early-stage prostate cancer patients

Wang, Binshuai; Song, Yimeng; Ge, Liyuan; Zhang, Shudong; Ma, Lulin

doi:10.1039/c8ra08682f

Cited by 7 publications

(7 citation statements)

References 28 publications

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“…The captured cells were detected by immunostaining method, achieving an LOD of 2 CTCs/4 mL blood (Fig. 2 ) [ 116 ]. An aptasensor using tetra(4-aminophenyl) porphyrin mediated reduced GO was constructed for the electrochemical detection of as low as 10 CTCs/mL [ 117 ].…”

Section: Nanomaterials Used In Liquid Biopsy Applicationsmentioning

confidence: 99%

Nanotechnology in emerging liquid biopsy applications

Kalogianni

2021

Nano Convergence

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Liquid biopsy is considered as the most attractive alternative to traditional tissue biopsies. The major advantages of this approach lie in the non-invasive procedure, the rapidness of sample collection and the potential for early cancer diagnosis and real-time monitoring of the disease and the treatment response. Nanotechnology has dynamically emerged in a wide range of applications in the field of liquid biopsy. The benefits of using nanomaterials for biosensing include high sensitivity and detectability, simplicity in many cases, rapid analysis, the low cost of the analysis and the potential for portability and personalized medicine. The present paper reports on the nanomaterial-based methods and biosensors that have been developed for liquid biopsy applications. Most of the nanomaterials used exhibit great analytical performance; moreover, extremely low limits of detection have been achieved for all studied targets. This review will provide scientists with a comprehensive overview of all the nanomaterials and techniques that have been developed for liquid biopsy applications. A comparison of the developed methods in terms of detectability, dynamic range, time-length of the analysis and multiplicity, is also provided.

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Section: Nanomaterials Used In Liquid Biopsy Applicationsmentioning

confidence: 99%

Nanotechnology in emerging liquid biopsy applications

Kalogianni

2021

Nano Convergence

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“…Reduced graphene oxide (rGO) films functionalized with anti-EpCAM and anti-prostate specific membrane antigen (anti-PSMA) antibodies have been recently fabricated by spray coating rGO solution onto a smooth glass slide. The antibody-modified rGO films exhibited a high efficiency (60%) of CTC capture from the blood of prostate cancer patients with prostate-specific antigen (PSA) levels of 4–10 ng mL −1 [ 67 ].…”

Section: Circulant Tumor Cells (Ctcs)mentioning

confidence: 99%

Graphene-Based Strategies in Liquid Biopsy and in Viral Diseases Diagnosis

et al. 2020

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Graphene-based materials are intriguing nanomaterials with applications ranging from nanotechnology-related devices to drug delivery systems and biosensing. Multifunctional graphene platforms were proposed for the detection of several typical biomarkers (i.e., circulating tumor cells, exosomes, circulating nucleic acids, etc.) in liquid biopsy, and numerous methods, including optical, electrochemical, surface-enhanced Raman scattering (SERS), etc., have been developed for their detection. Due to the massive advancements in biology, material chemistry, and analytical technology, it is necessary to review the progress in this field from both medical and chemical sides. Liquid biopsy is considered a revolutionary technique that is opening unexpected perspectives in the early diagnosis and, in therapy monitoring, severe diseases, including cancer, metabolic syndrome, autoimmune, and neurodegenerative disorders. Although nanotechnology based on graphene has been poorly applied for the rapid diagnosis of viral diseases, the extraordinary properties of graphene (i.e., high electronic conductivity, large specific area, and surface functionalization) can be also exploited for the diagnosis of emerging viral diseases, such as the coronavirus disease 2019 (COVID-19). This review aimed to provide a comprehensive and in-depth summarization of the contribution of graphene-based nanomaterials in liquid biopsy, discussing the remaining challenges and the future trend; moreover, the paper gave the first look at the potentiality of graphene in COVID-19 diagnosis.

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“…通过 EDC/NHS 活化 GO 表面含氧化学基团, 并与蛋白分子的氨基结合, 可以将牛血清白蛋白(BSA)共价修饰于 GO 表面(图 5) [87] . 此外, 还可以通过生物素-亲和素作用, 将生物素化的上皮细胞粘附分子抗体(anti-EpCAM)修饰到链霉亲和素化的 rGO 表面, 用于捕获循环肿瘤细胞 [88,89] .…”

Section: 多肽/蛋白修饰unclassified

Surface Chemical Modifications of Graphene Oxide and Interaction Mechanisms at the Nano-Bio Interface

Ma¹,

Xu²,

Liu³

2020

Acta Chim. Sinica

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Due to the unique physicochemical properties, graphene oxide has been widely applied in material chemistry, biomedical science and life science. However, here is still a great challenge to maximize the advantages of graphene oxide and overcome the deleterious effects caused by its inherent properties. For a better understanding of current status in this research field, recent progress in surface chemical modifications of graphene oxide and interaction mechanisms at the nano-bio interface has been comprehensively reviewed. First, the physicochemical properties of graphene oxide and the representative strategies of surface chemical modifications will be briefly introduced, including oxidation and reduction, carboxylation, amination, small organic molecule modification, polymer modification, peptide/protein modification, nucleic acid modification and nanoparticle modification, as well as their potential roles in mediating the graphene oxide-resulted biological effects. Following, we will present the primary interaction mechanisms of pristine and surface-modified graphene oxide at the nano-bio interface, including the formation of protein corona, cell membrane damage, membrane receptor interaction and oxidative stress. Finally, the knowledge gaps and future challenges in this research field will be detailedly discussed. Keywords graphene oxide; surface chemical modification; nano-bio interface; interaction mechanism; biological effect 1 引言 2004 年, Geim 等 [1] 首次通过机械剥离法制得单层石墨烯. 石墨烯(graphene)是一种二维纳米材料, 具有类似蜂巢的六元环平面网状结构, 厚度仅为 0.3354 nm [1] . 在石墨烯的纳米片层结构中, 碳原子发生了 sp 2 杂化, 每个碳原子通过键长为 142 nm 的 σ 键与其它三个碳原子连接, 并在与片层垂直的方向形成离域大 π 键 [2,3] . 这些结构特征赋予了石墨烯优异的机械强度、化学稳定性、高比表面积、导电和导热性能 [4][5][6][7] . 因此, 石墨烯在能源、材料、健康、环境等方面都展现了广阔的应用前景.石墨烯拥有多种衍生物, 包括氧化石墨烯(graphene oxide, GO)、还原氧化石墨烯(reduced graphene oxide, rGO)、氟化石墨烯(fluorographene)等.

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Antibody-modified reduced graphene oxide film for circulating tumor cell detection in early-stage prostate cancer patients

Abstract: We report the fabrication of an antibody-modified reduced graphene oxide film, which can be used to efficiently detect CTCs in PCa patients with PSA levels of 4–10 ng mL−1.

Cited by 7 publications

References 28 publications

Nanotechnology in emerging liquid biopsy applications

Nanotechnology in emerging liquid biopsy applications

Graphene-Based Strategies in Liquid Biopsy and in Viral Diseases Diagnosis

Surface Chemical Modifications of Graphene Oxide and Interaction Mechanisms at the Nano-Bio Interface

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