Antibody Nature of the Inhibitor to Antihaemophilic Globulin (Factor VIII)

Acquired Factor VIII Antibodies: Further Immunologic and Electrophoretic Studies

“…Acquired inhibitors of Factor VIII (antihemophilic globulin) have been shown to be antibodies (1)(2)(3). They are most commonly seen in multiply transfused patients with hereditary Factor VIII deficiency (hemophilia A), but are also observed occasionally in postpartum women, in patients with disease states associated with altered immunologic reactivity, and in some older individuals in prior good health (4).…”

A Specific Inhibitor of Human Clotting Factor V

“…Indirect support for the concept of the antibody character of circulating anticoagulants in most instances may be gained from the patient's history or from the physicochemical behaviour of the inhibitor. Direct serological evidence has been presented only in a few cases: occasionally precipitating antibodies against factor VIII or factor IX could be demonstrated 284 Lopez/P flugshaupt/Butler [4,6,11,12,18,19]; in other cases the inhibitor activity could be removed with a specific anti-immunoglobulin anti-scrum [2,16,1,7], To our knowledge, an inhibitor with antibody activity directed against factor V has never been demonstrated before. In our case, the antibody may be of autoimmune origin, although the question of aetiology has to remain open.…”

“…Chemical and physicochemical properties of the anticoagulant. The inhibitor was neither absorbable on aluminium-hydroxide nor on BaS04 [2]. It is not lipophilic since after shaking with ether, it was still found in the water phase.…”

Acquired Inhibitors of Coagulants

“…-Pa tien ts wi th inhi bi tors to Factor VI II were classified by Shapiro (29) into the following groups: (a) patients with classical haemophilia Aj (b) normal postpartum females within six months of deliveryj (c) individuals with a wide variety of diseases with immunological centrifugation in a sucrose gradient was similar to 7S 'Y-globulin. Bidwell and co-workers (33) showed that the inhibitor could be specifically neutralised by rabbit antibody to human 'YG-globulin and Shapiro & Carroll (34) re ported that light chain typing of samples from ten patients revealed only kappa chains. Lusher et al (26) likewise found their sample of inhibitor 'Y-globulin to be 'Y2K2 IgG on immunological typing and had previously [Lusher,Shuster & Poulik (35)] commented on the negative results in tests for passive cutaneous anaphylaxis consistent with the findings of Andersen & Terry (36), who confirmed that the light chains were of kappa type only and found that the heavy chains were 'YG.…”