Antibody prevalence and specificity to group C rotavirus in Swedish sera

Nilsson, Mikael; Sigstam, Gunnel; Svensson, Lennart

doi:10.1002/(sici)1096-9071(200002)60:2<210::aid-jmv17>3.0.co;2-7

Cited by 25 publications

(16 citation statements)

References 28 publications

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“…The seroprevalence of CHRV in Japan presented here (30%) is similar to the seroprevalences reported in Sweden (38%) (18), western New York State (30%) (24), and South Africa (34.4%) (28). However, it is slightly lower than the level found in Southampton, United Kingdom (43.4%) (11).…”

Section: Discussionsupporting

confidence: 86%

“…Seroepidemiological studies of group C rotaviruses have previously been carried out by indirect immunofluorescence tests (4,18,24) or ELISA-based tests (11,28,29). The ELISAbased tests were more appropriate for testing many serum samples.…”

Section: Discussionmentioning

confidence: 99%

“…In 1990, Oseto conducted the first serological survey of CHRV using an immune adherence hemagglutination assay with a human virus as antigen (21). Thereafter, seroepidemiological studies of group C rotaviruses have been undertaken by using reagents derived from porcine viruses (18,24,29) and human viruses (11,28). However, serological information about CHRV is still limited because of the lack of a constant supply of the virus antigen.…”

mentioning

confidence: 99%

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Seroepidemiology of Human Group C Rotavirus in Japan Based on a Blocking Enzyme-Linked Immunosorbent Assay

Kuzuya¹,

Fujii²,

Hamano³

et al. 2001

Clin Diagn Lab Immunol

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A novel blocking enzyme-linked immunosorbent assay (BL-ELISA) was developed for detection of antibodies to human group C rotavirus (CHRV). The specificity of the BL-ELISA was confirmed by using animal sera hyperimmunized to group A and group C rotaviruses and paired sera from five patients with acute CHRV gastroenteritis. Furthermore, there was concordance between the BL-ELISA and a neutralization assay for CHRV in 226 (95%) of 238 samples. By using the BL-ELISA, we determined the seroprevalence of CHRV in 704 serum samples obtained from nine different age groups of inhabitants of Okayama Prefecture, Japan, in 1992, 1994, and 1996. As a result, 211 sera (30%) were found to be positive for CHRV antibodies. The seroprevalence gradually increased with age and reached 52.7% in the oldest individuals. A further analysis of the youngest age group suggested that CHRVs predominantly prevail in persons older than 3 years of age in Japan. When comparing the three sampling years, a larger percentage of antibody-positive sera was detected in 1994 than in either 1992 or 1996 in individuals between 6 and 15 years of age, reflecting the occurrence of a CHRV outbreak among children during the winter of 1992 to 1993 that was previously documented. These results indicate that CHRV infections may occur more frequently in spite of the relatively low detection rate of the virus.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Seroepidemiology of Human Group C Rotavirus in Japan Based on a Blocking Enzyme-Linked Immunosorbent Assay

Kuzuya¹,

Fujii²,

Hamano³

et al. 2001

Clin Diagn Lab Immunol

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“…Group C school outbreaks in Japan, the UK and Sweden affected children 4-11 years of age and adults [Brown et al, 1989;Matsumoto et al, 1989;Nilsson et al, 2000b;Iizuka et al, 2006]. Seroepidemiological data also support an older age of first infection for group C rotavirus infection compared to group A rotavirus [Tsunemitsu et al, 1992;James et al, 1997;Nilsson et al, 2000a;Kuzuya et al, 2001;Castello et al, 2002;Iturriza-Gómara et al, 2004]. These results suggest that one cause of underestimation of group C rotavirus incidence is that most samples are collected from children below 3 years of age in many group A rotavirus gastroenteritis studies.…”

Section: Discussionmentioning

confidence: 99%

Detection and characterization of group C rotavirus in Buenos Aires, Argentina, 1997–2003

Castello¹,

Argüelles²,

Rota³

et al. 2009

Journal of Medical Virology

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The role of group C rotaviruses as a cause of diarrhea was examined among children <17 years of age admitted to a Hospital in a suburban area of Buenos Aires, Argentina between 1997 and 2003. A total of 1,579 fecal samples were screened for group A (RVA) and C (RVC) rotaviruses by two in-house ELISA methods at Quilmes University (UNQ-ELISA). Samples positive, doubtful and negative by RVC specific UNQ-ELISA (n = 246) were examined further for RVC by another in-house ELISA (CDC-ELISA), electron microscopy, RT-PCR, nested PCR, and Southern hybridization. Sensitivity, specificity, and predictive values for each test were determined. While the sensitivity was comparable for the nested PCR and CDC-ELISA methods (82.5%), the molecular methods were slightly more specific. Poorly preserved particles were often seen in fecal samples, suggesting that degradation of RNA could be a factor influencing the performance of molecular methods. The incidence of RVC was estimated to be 3% without apparent differences among seasons. RVC infected patients had a significantly (P < 0.001) higher median age (6 years vs. 1 year) than those with RVA infection. Sequence of the RVC VP7 gene from six Argentinean strains and sequences reported previously in different countries showed high nucleotide (94.4-99.9%) sequence identities, indicating a high degree of conservation for human RVC VP7 genes among strains collected on five continents over a period of 17 years. These findings indicate that RVC is a significant cause of diarrhea and it is necessary to develop simple and sensitive serological methods for its detection.

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“…In other countries serological surveys have suggested that group C rotavirus infections are far more common than actual virus identification may have indicated (2,7,13,19). These surveys, however, also included adults, among whom there was usually a higher level of group C rotavirus seropositivity than among infants and young children.…”

Section: Discussionmentioning

confidence: 99%

Incidence of Group C Human Rotavirus in Central Australia and Sequence Variation of the VP7 and VP4 Genes

et al. 2004

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Human group C rotavirus was identified in central Australia in each of eight years over a 16-year period between 1982 and 1997. Cases occurred either sporadically but over a relatively short period of time or as clustered outbreaks. These are the only reports of human group C rotavirus in Australia other than that of a single case reported approximately 1,800 km away in 1982. The electrophoretic genome profiles of isolates were identical for all those identified within the same year but different between those identified in different years. The VP7 genes of four isolates identified in four different years over a 7-year period between 1987 and 1993, and the VP4 genes of two of these isolates showed relatively little variation in genome and deduced amino acid sequence upon comparison of the equivalent genes between isolates. The sequences were also very similar to those from the corresponding genes from most of the human group C rotavirus isolates from other countries. This continues the observation of a high degree of gene sequence conservation among human group C rotaviruses worldwide.

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Antibody prevalence and specificity to group C rotavirus in Swedish sera

Cited by 25 publications

References 28 publications

Seroepidemiology of Human Group C Rotavirus in Japan Based on a Blocking Enzyme-Linked Immunosorbent Assay

Seroepidemiology of Human Group C Rotavirus in Japan Based on a Blocking Enzyme-Linked Immunosorbent Assay

Detection and characterization of group C rotavirus in Buenos Aires, Argentina, 1997–2003

Incidence of Group C Human Rotavirus in Central Australia and Sequence Variation of the VP7 and VP4 Genes

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