Antibody Production by Nonimmune Spleen Cells Incubated with RNA from Immunized Mice

Abstract

RNA isolated from the spleens of B(6)AF(1) mice immunized to sheep red blood cells was incubated with the spleen cells of nonimmunized mice. The number of antibody-forming cells detected by the Jerne plaque technique was significantly higher among the cells incubated with the RNA from immune animals than among cells incubated without RNA, or with RNA from nonimmune animals.

The Effect of Antimacrophage Serum on Macrophage-Lymphocyte Interaction in Vitro

“…The importance of macrophages in the development of a primary immune response in vitro has been demonstrated previously (1)(2)(3)(4). Hersh and Harris have shown (5) that macrophages are necessary for the secondary immune response to PPD and SKSD in vitro.…”

Production of cytotoxic antibody to a benz(a)pyrene-induced sarcoma in mice receiving xenogeneic antitumor immune RNA

“…58, D-6900 Heidelberg, Federal Republic of Germany. Dr. Fritze was the recipient of a postdoctoral fellowship from the Deutsche Forschungsgemeinschaft Reprint requests should be addressed to: Dr. D. Fritze at the above address show, using a variety of antigens, that normal, nonimmune lymphoid cells can be converted to specific immunologic reactivity by treatment with extracts rich in RNA prepared from macrophages or other lymphoid cells previously exposed to antigen [4,10,17,18]. Normal nonimmune lymphoid cells incubated in such RNA preparations in vitro were subsequently shown to mediate a variety of cellular and humoral immune responses both in vivo and in vitro.…”

Transfer of tumor immunity with ribonucleic acid

“…8 s 9 and to cause the formation of zones of specific hemolysis in gels. 6,10 In addition, such RNAincubated lymphoid cells have been shown to produce inhibition of macrophage migration in capillary tubes when exposed to specific antigen,l3,34 and to mediate allograft imIn the prescnt communication, we report studies performed in 2 different tumor-host systems in which antitumor immunoreactivm~inity.15. 22, 23 ity was conferred upon normal, nonimmune lymphoid cells by incubation in vitro with RNA-rich preparations extracted from the lymph nodes and spleens of animals which had been immunized with the tumor to be studied.…”

“…Co., Waltham, Mass. ), and sonication was carried out until 95% of the cell count was eliminated, usually in [5][6][7] minutes. T h e suspension was then centrifuged at 100,000 x g for one hour.…”