Antibody Production in Milk Serum After Virus Instillation of Goat Mammary Gland: Ii. Biochemical Isolation and Purification of Antibody to Influenza Virus

Abstract

Goat 111ilk was obtained from the right lactiferous sinus following instillation of PR8 influenza type A virus. The proteins precipitated from goat milk serum by (NH.1)aSO.a were found to contain antibody activity ancl further purification was undertaken. I n the clevelopment of the method the protcin solutions were subjected to paper chromatography and paper and polyucetate high voltage electrophoresis, both before and after a number of isolation a~~d purilication steps. In addition, preparations from each step were run on thc 'Tcclinicon amino acid analyzer for the presence of amino acicls and (or) peptides.As a working procedure, a preparation obtained after cold acetone precipitation was subjected to rivanol treatment, and after electrophoresis a single protein band containing the antibody was obtained. This ~natcrial was further purified by gel filtration using a Bio-Rad P150 column. 'l'he antibod), containing proteln was bnally isolated in the eluate by a fractio~i collector using phosphate buffer as the eluting fluid. There was escellent recovery of the antibody activity In each preparation.

Immunofluorescent studies of the local immune response in the mammary glands of rats

“…Apart from bacteria, viruses represent another type of agent which may cause mastitis. Natural and experimental viral infections of the mammary gland have been examined in cows (9, 10), sows (3,4), and goats (24,27,28). Immunohistochemical studies concerning the appearance of immunocytes in the mammary gland after virusinduced mastitis appear to be lacking.…”