Antibody Response in Children Infected with Giardia intestinalis before and after Treatment with Secnidazole

Jiménez, Juan Carlos; Pinon, Anthony; Dive, Daniel; Capron, Moníque; Dei‐Cas, Eduardo; Convit, J

doi:10.4269/ajtmh.2009.80.11

Cited by 15 publications

(7 citation statements)

References 24 publications

(25 reference statements)

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“…Also, Guimarães et al determined by electrophoretic profile a fraction of Brazilian isolates, reporting the presence of 16 bands with molecular weights between 122 and 10kDa [11]. More recently, our group identified in the soluble extract bands between 137 and 14kDa reacting with the specific IgA anti-Giardia in the serum of infected children [13]. In the present work, were detected new proteins for specific IgA antibody response against G. intestinalis antigens in the fractions with reactivity for proteins of 205, 176, 110kDa, a region between 76 to 41kDa and the band of 23kDa which were mainly recognized in the fraction 2.…”

Section: Discussionsupporting

confidence: 53%

“…Individuals with active giardiasis display higher levels of specific IgG but lower levels of IgM than do subjects free of established infection, and raised levels of Giardia specific IgG and IgA have been correlated with the presence or absence of parasites [8,9]. These and other investigators have quantified the levels of IgG, IgM, and IgE responses to different antigens [10][11][12], but curiously, there have been fewer reports in the literature on the levels of IgA antibody responses in human giardiasis [13]. Investigations have indicated the protective role of the specific IgA antibodies [14,15].…”

Section: Introductionmentioning

confidence: 99%

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Parasite-specific IgA response in infected children by Giardia intestinalis from rural communities of Venezuela.

Durán¹,

Jiménez²,

Pocaterra³

et al. 2018

J Parasit Dis Diagn Ther

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Giardiasis is an infection caused by Giardia intestinalis into the small intestine that affects children in school age. The aim of the work was evaluated the specific IgA anti-Giardia antibodies in serum of children from rural communities were evaluated 100 children of 3 to 13 years old and measurement the levels of IgA antibody response against immunodominant antigens of Giardia intestinalis by ELISA and Westernblot. The soluble extract of Giardia intestinalis and fractions were obtained by differential centrifugation. Electrophoretic pattern showed several proteins from 205 to 14kDa, and sera of infected children recognized proteins of 205, 176, a region from 76 to 41kDa, and a band of 23kDa in the fraction 2. The IgA antibody response anti-Giardia for to soluble extract was higher in infected children compared to uninfected children (0.29 ± 0.16 vs. 0.07 ± 0.04; p ≤ 0.05). The IgA antibody response against the fraction 2 was higher in infected group than controls (1.0 ± 0.2 vs. 0.75 ± 0.26; p ≥ 0.05), the fraction 3 showed similar response to the soluble extract (0.45 ± 0.12 vs. 0.3 ± 0.12; p ≥ 0.05). The infection by Giardia intestinalis produce specific IgA antibodies directed to the proteins of the fraction 2. These proteins could be tested as antigens for serological diagnosis of giardiasis.

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Section: Discussionsupporting

confidence: 53%

Section: Introductionmentioning

confidence: 99%

Parasite-specific IgA response in infected children by Giardia intestinalis from rural communities of Venezuela.

Durán¹,

Jiménez²,

Pocaterra³

et al. 2018

J Parasit Dis Diagn Ther

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“…Infection with G. duodenalis is one of the most common human infections, particularly in developing countries, which affects mainly preschool children and school children (Jiménez et al 2009 Table 1 The clinical and demographic data of studied population Group I (Giardia-parasitized group), n022…”

Section: Discussionmentioning

confidence: 99%

Saliva and sera IgA and IgG in Egyptian Giardia-infected children

et al. 2012

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Giardiasis is a gastrointestinal infection of wide distribution that is more prevalent in childhood. Easy and rapid diagnosis of giardiasis is essential for reduction of this infection. This cross-sectional study included 62 children in which collection of saliva, stool and serum samples was performed. An enzyme-linked immunosorbent assay (ELISA) technique was evaluated to detect IgA and IgG responses in both saliva and serum samples. Twenty-two children were positive for Giardia duodenalis infection by direct examination of faecal specimens, 20 non-infected and 20 infected with other parasites. Salivary and serum IgA and IgG responses against G. duodenalis infection were significantly higher in Giardia parasitized than non-Giardia parasitized children (p < 0.001). This concludes that specific salivary IgA may serve as a diagnostic tool and specific salivary IgG as a screening tool in monitoring the exposure of various populations to Giardia duodenalis. The advantage of salivary assays over serum immunoglobulin assay is being easy and non-invasive in sampling technique which is important especially for young children.

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“…Testing these hypotheses requires data on the affinity and avidity of maternal antibodies to parasite antigens exposed during infections in offspring. It, therefore, remains unclear whether, in adults, a protective response is induced after treatment against parasites, how it differs from acquired immunity to ‘natural’ infection [48,49] and how this affects levels of protection conferred to offspring [50]. …”

Section: Discussionmentioning

confidence: 99%

Drug treatment of malaria infections can reduce levels of protection transferred to offspring via maternal immunity

et al. 2012

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Maternally transferred immunity can have a fundamental effect on the ability of offspring to deal with infection. However, levels of antibodies in adults can vary both quantitatively and qualitatively between individuals and during the course of infection. How infection dynamics and their modification by drug treatment might affect the protection transferred to offspring remains poorly understood. Using the rodent malaria parasite Plasmodium chabaudi, we demonstrate that curing dams part way through infection prior to pregnancy can alter their immune response, with major consequences for offspring health and survival. In untreated maternal infections, maternally transferred protection suppressed parasitaemia and reduced pup mortality by 75 per cent compared with pups from naïve dams. However, when dams were treated with anti-malarial drugs, pups received fewer maternal antibodies, parasitaemia was only marginally suppressed, and mortality risk was 25 per cent higher than for pups from dams with full infections. We observed the same qualitative patterns across three different host strains and two parasite genotypes. This study reveals the role that within-host infection dynamics play in the fitness consequences of maternally transferred immunity. Furthermore, it highlights a potential trade-off between the health of mothers and offspring suggesting that anti-parasite treatment may significantly affect the outcome of infection in newborns.

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Antibody Response in Children Infected with Giardia intestinalis before and after Treatment with Secnidazole

Cited by 15 publications

References 24 publications

Parasite-specific IgA response in infected children by Giardia intestinalis from rural communities of Venezuela.

Parasite-specific IgA response in infected children by Giardia intestinalis from rural communities of Venezuela.

Saliva and sera IgA and IgG in Egyptian Giardia-infected children

Drug treatment of malaria infections can reduce levels of protection transferred to offspring via maternal immunity

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