Antibody response to 14-valent pneumococcal capsular polysaccharide vaccine in pre-school age children

Leinonen, Maija; Säkkinen, A; Kalliokoski, Raili; Luotonen, Jukka; Timonen, M.; Mäkelä, P. Helena

doi:10.1097/00006454-198601000-00008

Cited by 135 publications

(59 citation statements)

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“…This finding confirmed our observations in humanized SCID/SCID mice transplanted with human IgM memory B lymphocytes and immunized with soluble polysaccharides. Serotype 3 is known to be highly immunogenic and even immunogenic at earlier age (from 3 mo of age) (35,36, and own unpublished data). We also previously found that an inadequate response to serotype 3 predicts unresponsiveness to other serotypes (20).…”

Section: Discussionmentioning

confidence: 86%

Human Memory B Lymphocyte Subsets Fulfill Distinct Roles in the Anti-Polysaccharide and Anti-Protein Immune Response

Moens

Wuyts

Meyts

et al. 2008

The Journal of Immunology

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There is controversy on the role of IgM memory and switched memory B lymphocytes in the Ab response to T cell-independent and T cell-dependent Ags. We transplanted SCID/SCID mice with human B lymphocyte subsets and immunized them with heat-inactivated Streptococcus pneumoniae or with a pneumococcal vaccine. Inactivated S. pneumoniae and soluble pneumococcal capsular polysaccharides elicited an IgM anti-polysaccharide and anti-protein Ab response from IgM memory B lymphocytes and an IgG anti-polysaccharide and anti-protein response from switched memory B lymphocytes. In addition to the IgM Ab response, IgM memory B cells elicited an IgG anti-polysaccharide and anti-protein Ab response after immunization with inactivated S. pneumoniae or soluble pneumococcal capsular polysaccharides. In conclusion, our findings provide evidence for a versatile role of IgM memory B cells in T-independent and T-dependent immune responses.

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Section: Discussionmentioning

confidence: 86%

Human Memory B Lymphocyte Subsets Fulfill Distinct Roles in the Anti-Polysaccharide and Anti-Protein Immune Response

Moens

Wuyts

Meyts

et al. 2008

The Journal of Immunology

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“…The use of whole 'Pneumovax' containing 23 polysaccharides as a coating antigen in ELISA assays to assay pneumococcal antibodies may be criticised on the grounds that responses to strongly immunogenic individual serotypes such as type 3 may mask poor responses to other serotypes [28]. Whilst this is an important consideration in the assessment of protective levels of serotype-specific pneumococcal antibodies in epidemiological studies, it is unlikely to be an important confounding factor when test immunization is used as an investigatory tool in the assessment of possible immunodeficiency.…”

Section: Discussionmentioning

confidence: 99%

Antipolysaccharide antibodies in 450 children with otitis media

Misbah¹,

Griffiths²,

Mitchell

et al. 1997

Clinical and Experimental Immunology

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SUMMARYWe have measured antibodies to pneumococcal and Haemophilus polysaccharides in a prospective study of 450 children aged 2-16 years with otitis media requiring grommets (ear tubes). Pneumococcal antibody levels were significantly higher in the 2-6 year (P < 0 . 004) and 7-10 year (P < 0 . 04) study groups in comparison with age-matched controls. There was no difference in Haemophilus antibody levels between the study and control group children for the age groups 2-6 years and 11-16 years. Haemophilus antibody levels were significantly lower in the 7-10 year (P < 0 . 003) group in comparison with age-matched controls. Eighty-eight out of 450 (19 . 6%) children had pneumococcal antibody levels below the 25th percentile. Nineteen out of 88 (21 . 6%) children with pneumococcal antibody levels below the 25th centile were test immunized with 23 valent Pneumococcal polysaccharide and unconjugated Haemophilus type b capsular polysaccharide. Of these 19 children (aged 4-11 years), five mounted suboptimal responses to both polysaccharide antigens, whilst one child failed to respond to Haemophilus polysaccharide alone. There was no significant difference in the prevalence of IgG subclass deficiency between the normal responders and poor responders to immunization (P = 0 . 12). We found no evidence of specific polysaccharide antibody deficiency in the vast majority of the 450 children studied. However, the significance of poor antibody responses to test immunization in a small minority of children with otitis media is unclear. Long-term follow up of these children is required to determine whether poor immunization responses herald the development of frank antibody deficiency.

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“…In general, maturation of the human antibody response against capsular polysaccharides of pediatric serotypes is slow. This holds true in particular for type 6 pneumococci, for which maturation of the antibody response is the slowest of all the serotypes (4,15). Moreover, type 6 pneumococci are frequently associated with antibiotic resistance (5,18), and recently vancomycin tolerance in S. pneumoniae has been described (17).…”

mentioning

confidence: 98%

Synthetic 6B Di-, Tri-, and Tetrasaccharide-Protein Conjugates Contain Pneumococcal Type 6A and 6B Common and 6B- Specific Epitopes That Elicit Protective Antibodies in Mice

et al. 2001

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The immunogenicity and protective capacity of Streptococcus pneumoniae 6B capsular polysaccharide (PS)-derived synthetic phosphate-containing disaccharide (Rha-ribitol-P-), trisaccharide (ribitol-P-Gal-Glc-), and tetrasaccharide (Rha-ribitol-P-Gal-Glc-)-protein conjugates in rabbits and mice were studied. In rabbits, all saccharides conjugated to keyhole limpet hemocyanin (KLH) evoked high levels of pneumococcal (Pn) type 6B antibodies that facilitated type-specific phagocytosis. Unlike the disaccharide rabbit antisera, tri-and tetrasaccharide rabbit antisera also reacted with 6A PS in an enzyme-linked immunosorbent assay (ELISA) and promoted phagocytosis of 6A pneumococci. All these rabbit antisera passively protected mice against a Pn 6B challenge. The disaccharide conjugate-induced antiserum, however, failed to protect mice against a 6A challenge. In mice, phagocytic and protective anti-Pn 6B antibodies were only induced by the tetrasaccharide conjugate and not by PS 6B or PS 6B-protein conjugates. These antibodies did not cross-react with 6A PS in ELISA and were unable to phagocytize 6A pneumococci. In conclusion, the disaccharide and tetrasaccharide conjugates already contain epitopes capable of inducing 6B-specific, fully protective antibodies in rabbits and mice, respectively.

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Antibody response to 14-valent pneumococcal capsular polysaccharide vaccine in pre-school age children

Cited by 135 publications

References 0 publications

Human Memory B Lymphocyte Subsets Fulfill Distinct Roles in the Anti-Polysaccharide and Anti-Protein Immune Response

Human Memory B Lymphocyte Subsets Fulfill Distinct Roles in the Anti-Polysaccharide and Anti-Protein Immune Response

Antipolysaccharide antibodies in 450 children with otitis media

Synthetic 6B Di-, Tri-, and Tetrasaccharide-Protein Conjugates Contain Pneumococcal Type 6A and 6B Common and 6B- Specific Epitopes That Elicit Protective Antibodies in Mice

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