Antibody response to cytomegalovirus polypeptides captured by monoclonal antibodies on the solid phase in enzyme immunoassays

Abstract

Antibodies to different cytomegalovirus (CMV) polypeptide antigens, captured by monoclonal antibodies coated on the solid phase of an enzyme immunoassay test, were analyzed in 42 serum pairs submitted for serodiagnosis of CMV infection. Three CMV antigens, captured on the solid phase by three monoclonal antibodies of different specificities, designated CH92-1, CH65-1, and CH16-1, were glycoproteins A (gA), gC, and gD, respectively; and one antigen, captured by CH23, was a polypeptide with an apparent molecular weight of 150,000, possibly associated with the nucleocapsid. Of these four CMV antigens, gA captured by CH92-1 was most effective in eliciting an antibody response. Antibody to this antigen was present in serum samples at a higher concentration in primary and reactivated infection and persisted longer than did antibody to the other tested antigens. In contrast, antibody to antigen captured by CH23 was at a lower concentration, rose more slowly in infection, and persisted for a shorter time than did antibody to the other antigens. Antibody response to gC and gD was intermediate in concentration and temporal appearance compared with the antibody response to gA and to the polypeptide bound by CH23. An enzyme immunoassay on paired serum samples with the captured glycoproteins as antigen was equal for the detection of current infection to an enzyme immunoassay with the whole CMV antigen from infected cell lysates. Enzyme immunoassays with either the CMV glycoproteins or the whole CMV antigen from infected cell lysates were superior to a complement fixation test with a glycine extract antigen for serodiagnosis of current infection.

Serum Antibodies to Individual Cytomegalovirus Structural Polypeptides in Renal Transplant Recipients during Viral Infection

“…Among them p150 was recognized by IgG present in the zero-time serum samples of all the patients with no correlation with the antibody levels detected by conventional serological procedures. The data obtained in the present investigation define that p150 is a good antigen in vivo as already suggested (3,9), and induces a long-lasting antibody response. Therefore, the presence of antibody against this polypeptide could be a very useful tool to assess the occurrence of a previous CMV infection.…”

Humoral immune response against human cytomegalovirus (HCMV)-specific proteins after HCMV infection in lung transplantation as detected with recombinant and naturally occurring proteins

“…Antibodies specific for all four recombinant proteins could be detected in only two patients, and two patients produced an-VOL. 2,1995 HUMORAL IMMUNE RESPONSES TO HCMV 215 tibodies to p52 alone. The highest IgM antibody levels found appeared to be directed against p52 and pp65.…”

“…However, one patient who had a VOL. 2,1995 HUMORAL IMMUNE RESPONSES TO HCMV 217 maximum antigenemia test of 4 positive cells per 50,000 leukocytes, which is very low for a primary CMV infection, had an early antibody response as tested in the recombinant proteinbased ELISA, predominantly to pp65 and IE2, both IgM and IgG classes. An interesting finding was that in patients with secondary CMV infections, the level of antibodies to linear epitopes was inversely correlated to the level of antigenemia but the level of antibodies to conformational epitopes was not.…”

“…To investigate humoral immune responses to CMV, different techniques like Western blotting (immunoblotting) (3,4,10) and various enzyme-linked immunosorbent assays (ELISAs) have been used (2,14). The mixtures of antigens used as substrates in these various assays were derived from CMV virions or from CMV-infected fibroblasts and may have been prepared by different procedures, thereby possibly leading to different findings and conclusions.…”

Antibody response to human cytomegalovirus (HCMV) glycoprotein B (gB) in AIDS patients with HCMV end-organ disease

“…HCMV glycoprotein B (gB) is a major component of the viral envelope that plays a central role in viral entry and in virus spread from cell-to-cell . In addition, gB is highly immunogenic in humans [Cremer et al, 1985;Gonczol et al, 1986Gonczol et al, , 1990Kniess et al, 1991;Navarro et al, 1997;Pereira et al, 1991] and elicits a dominant antiviral neutralizing response [Britt et al, 1988[Britt et al, , 1990Marshall et al, 1992Marshall et al, , 1994Rasmussen et al, 1991]. Consequently, specific anti-gB antibodies are likely to be involved in protection against HCMV.…”