Antibody response to Nocardia brasiliensis antigens in man

Salinas-Carmona, Mario C.; Vera, Lucio; Welsh, Oliverio; Rodrı́guez, Manuel

doi:10.1016/s0934-8840(11)80546-3

Cited by 19 publications

(13 citation statements)

References 19 publications

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“…Salinas-Carmona et al previously developed an experimental model of chronic intracellular infection in BALB/c mice by injecting N. brasiliensis ATCC 700358 in order to induce an actinomycetoma lesion (11). Immunodominant antigens from N. brasiliensis were identified in sera from infected mice as well as human patients with actinomycetoma (13). These immunodominant antigens included P61, P26, and P24, which were isolated and purified from N. brasiliensis cells (17).…”

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Humoral Immunity through Immunoglobulin M Protects Mice from an Experimental Actinomycetoma Infection byNocardia brasiliensis

Salinas-Carmona

Pérez-Rivera

2004

Infect Immun

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An experimental model of infection with Nocardia brasiliensis, used as an example of a facultative intracellular pathogen, was tested. N. brasiliensis was injected into the rear foot pads of BALB/c mice to establish an infection. Within 30 days, infected animals developed a chronic actinomycetoma infection. Batch cultures of N. brasiliensis were used to purify P61, P38, and P24 antigens; P61 is a catalase, and P38 is a protease with strong caseinolytic activity. Active and passive immunizations of BALB/c mice with these three purified soluble antigens were studied. Protection was demonstrated for actively immunized mice. However, immunity lasted only 30 days. Other groups of immunized mice were bled at different times, and their sera were passively transferred to naive recipients that were then infected with N. brasiliensis. Sera collected 5, 6, and 7 days after donor immunization conferred complete, long-lasting protection. The protective effect of passive immunity decreased when sera were collected 2 weeks after donor immunization. However, neither the early sera (1-, 2-, and 3-day sera) nor the later sera (30-or 45-day sera) prevented the infection. Hyperimmune sera with the highest levels of immunoglobulin G (IgG) to N. brasiliensis antigens did not protect at all. The antigens tested induced two IgM peaks. The first peak was present 3 days after immunization but was not antigen specific and did not transfer protection. The second peak was evident 7 days after immunization, was an IgM response, was antigen specific, and conferred protection. This results clearly demonstrate that IgM antibodies protect the host against a facultative intracellular bacterium.

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confidence: 99%

Humoral Immunity through Immunoglobulin M Protects Mice from an Experimental Actinomycetoma Infection byNocardia brasiliensis

Salinas-Carmona

Pérez-Rivera

2004

Infect Immun

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“…Even though many persons are accidentally inoculated, few develop the actinomycetoma lesion; host mechanisms that control and heal the lesion are unknown. Anti-N. brasiliensis antibodies have been demonstrated both in human patients and in experimental animals (15,16). The role of these antibodies in host protection is not clear (2,17); in humans, the presence of anti-N. brasiliensis antibodies has been helpful in serodiagnosis and has recently been introduced for use in routine clinical laboratories (18).…”

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Immune Response toNocardia brasiliensisAntigens in an Experimental Model of Actinomycetoma in BALB/c Mice

et al. 1999

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Nine- to twelve-week-old BALB/c mice were injected in footpads with 107 CFU of a Nocardia brasiliensis cell suspension. Typical actinomycetoma lesions, characterized by severe local inflammation with abscess and fistula formation, were fully established by day 28 after infection. These changes presented for 90 days, and then tissue repair with scar formation slowly appeared, with complete healing after 150 days of infection. Some animals developed bone destruction in the affected area. Histopathology showed an intense inflammatory response, with polymorphonuclear cells and hyaloid material around the colonies of the bacteria, some of which were discharged from draining abscesses. Sera from experimental animals were analyzed by Western blotting, and immunodominant antigens P61 and P24 were found as major targets for antibody response. Anti-P24 immunoglobulin M (IgM) isotype antibodies were present as early as 7 days, IgG peaking 45 days after infection. Lymphocyte proliferation with spleen and popliteal lymph node cells demonstrated thymidine incorporation at 7 days after infection, the stimulation index decreasing by day 60. Levels of interleukin-1 (IL-1), IL-2, IL-4, IL-6, tumor necrosis factor alpha, and gamma interferon (IFN-γ) were determined by enzyme-linked immunosorbent assay in the sera of infected animals. The circulating levels of IFN-γ increased more than 10 times the basal levels; levels of IL-4, IL-6 and IL-10 also increased during the first 4 days of infection.

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“…P61 could be related to the 65-kDa protein from Mycobacterium tuberculosis that has been demonstrated to share epitopes with some other bacteria, e.g., N. asteroides, Mycobacterium leprae, and Eschenichia coli (25). The relationship of the M. leprae protein with P61 of N. brasiliensis is supported by their close molecular weights and the slight recognition of P61 by sera from leprosy patients in Western blot assays (21).…”

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confidence: 94%

Isolation and purification of two immunodominant antigens from Nocardia brasiliensis

et al. 1992

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Two immunogenic proteins from a crude extract of Nocardia brasiliensis were purified to homogeneity. A 61-kDa protein (P61) was isolated from a 50% ammonium sulfate precipitate in two steps. Initially, P61 was obtained by electroelution in a 10%v nondenatured preparative polyacrylamide gel electrophoresis (PAGE). In a second step, the eluate from the nondenatured gel was run in a 12% sodium dodecyl sulfate (SDS) preparative polyacrylamide gel. After elution, a single band was demonstrated by SDS-PAGE and Western blot (immunoblot). Also, a 24-kDa immunogenic protein (P24) was isolated by gel filtration in a Sephadex G-100 column and then by electroelution in a 12% nondenatured polyacrylamide gel. In a previous paper, we showed by Western blot assays that these proteins are recognized by the sera of mycetoma patients and not by sera from mycobacterial-infected or healthy individuals. We consider these proteins to be good candidates for the study of the host-parasite relationship in nocardial infections. The possible clinical application of these purified antigens in a serological diagnosis is discussed.

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Antibody response to Nocardia brasiliensis antigens in man

Cited by 19 publications

References 19 publications

Humoral Immunity through Immunoglobulin M Protects Mice from an Experimental Actinomycetoma Infection byNocardia brasiliensis

Humoral Immunity through Immunoglobulin M Protects Mice from an Experimental Actinomycetoma Infection byNocardia brasiliensis

Immune Response toNocardia brasiliensisAntigens in an Experimental Model of Actinomycetoma in BALB/c Mice

Isolation and purification of two immunodominant antigens from Nocardia brasiliensis

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